Xylitol production from xylose by a self-isolated furfural and 5-hydroxymethyl furfural assimilating Pichia guilliermondii was studied under oxygen limitation. An extremely low initial volumetric oxygen transfer coefficient (0.075 h(-1)) was found most favorable to the xylitol production with yield of 0.61 g g(-1). Related enzymes activities were also investigated and discussed. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.