| 초록 |
Chitosan are widely accepted in the context of non-viral gene delivery. Chitosan, a nature cationic polysaccharide, has been considered as a good candidate for gene delivery systems. In this study, to develop chitosan-based efficient gene delivery systems, chitosan oligosaccharides (COS) with various molecular weight (3~45 kDa) and narrow molecular weight distributions (PDI, 1.1 ~ 1.3) were prepared and their MW-property relationships with plasmid DNA, showing MW-dependent gene condensations. The in vitro gene transfections revealed that COS-mediated gene deliveries are strongly affected by the MW of COS, COS/DNA ratios, and pH of the transfection medium. The COS9 (MW 9000 Da), the most efficient fraction, showed Lipofectamine compatible high gene transfections at the carrier/DNA weight ratio of 30 in the transfection medium pH of 6.5 or below. Therefore, with its excellent safety profiles, fraction COS (COS9) may be considered a potential candidate for efficient gene carrier. |
