화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Applied Chemistry for Engineering, Vol.23, No.5, 496-500, October, 2012
Export Citation
자외선 조사에 의해 유도된 미세조류 Arthrospira platensis 변이주의 특성
Characterization of Arthrospira platensis Mutants Generated by UV-B Irradiation
박현진1, 김영화2, 이재화1, 2, †
  • 1신라대학교 의생명과학대학 생명공학과
  • 2신라대학교 의생명과학대학 제약공학과
Hyun-Jin Park1, Young-Hwa Kim2, and Jae-Hwa Lee1, 2, †
  • 1Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University, Busan 617-736, Korea
  • 2Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University, Busan 617-736, Korea
E-mail:
초록
Arthrospira platensis (A. platensis)는 상업적으로 중요한 사상형 미세조류이다. 화학적 혹은 물리적 돌연변이원에 의해 유도된 변이주 분리는 균주 개량에 중요하게 작용한다. 본 연구에서, A. platensis에서 자외선(UV-B)조사에 따른 영향을 살펴보았고, 그에 따른 변이주를 확보하였다. A. platensis를 자외선(15 Watt, 254 nm)을 이용하여 1, 3, 5, 10 min 동안처리 한 후, 얻어진 변이주를 각각 UM1, UM3, UM5, UM10으로 명명하였다. 특히, UM5 변이주는 야생균주와 비교하여 지질의 함량이 8∼11배 가량 크게 증가하였다. 또한, 카로티노이드 함량과 항산화 효소(peroxidase, superoxide dismutase) 활성이 증가하였다. 이 결과, 자외선에 의해 유도된 변이주는 생리활성 물질을 축적하였으며, 이러한 유용성분을 생산하는 미세조류의 균주 개발은 미세조류의 산업화를 촉진시킬 것으로 기대된다.
Arthrospira platensis (A. platensis) is an economically important filamentous microalgae. The isolation of mutants by chemical or physical mutagen is a clue for the strain improvement. In this study, effects of ultraviolet-B (UV-B) radiation on A. platensis were investigated. Cells (or microalgae) were exposed to UV-B (15 Watt, 254 nm) for 1, 3, 5, and 10 min, and resulting mutants were designated UM1, UM3, UM5, and UM10, respectively. Especially, the lipid content of UM5 mutant was considerably increased by 8∼11 fold compared to that of wild types. Moreover, the carotenoid content and antioxidant enzyme (peroxidase and superoxide dismutase) activity were increased. It was shown that UV-induced mutants can accumulate bioactive compounds, which will be useful for the industrial production of valuable products.
Keywords:microalgae;Arthrospira platensis;UV-B;mutant
  1. Dong SJ, Kim KW, Cho SY, Kor. J. Fish. Aquat. Sci., 44, 1 (2011)
  2. Oh HM, Choi AR, Mheen TI, Kor. J. Microbiol.Biotechnol., 31, 95 (2003)
  3. Choi GG, Bae MS, Park JS, Park BJ, Ahn CY, Oh HM, Kor. J. Microbiol. Biotechnol., 35, 45 (2007)
  4. Cha SH, Kim MJ, Yang HY, Jin CB, Jeon YJ, Oda T, Kim DK, Kor. J .Fish Aquat Sci., 43, 437 (2010)
  5. Jo BH, Cha HJ, KSBB J., 25, 109 (2010)
  6. Spolaore P, Cassan CJ, Duran E, Isambert A, J. Biosci.Bioeng., 1, 87 (2006)
  7. Sung KD, Ann JH, Lee JY, Ohh SJ, Lee HY, Kor.J. Biotechnol. Bioeng., 10, 401 (1995)
  8. Park BJ, Kim BM, Shim SH, Kim JD, Lee CG, Kor. J. Microbiol. Biotechnol., 34, 136 (2006)
  9. Yoo C, Kim CJ, Choi GG, Ahn CY, Choi JS, Oh HM, Microbiol. Soc. Kor., 45, 268 (2009)
  10. Schneider JC, Livne A, Sukenik A, Roessler PG, Cell culture biotechnol., 40, 807 (1995)
  11. Chaturvedi R, Uppalapati SR, Alamsjah MA, Fujita Y, J. Appl. Phycol., 16, 135 (2004)
  12. Kamath BS, Vidhyavathi R, Sarada R, Ravishankar GA, Bioresour. Technol., 99(18), 8667 (2008)
  13. Cordero BF, Obraztsova I, Couso I, Leon R, Vargas MA, Rodriguez H, Mar. Drugs., 9, 1607 (2011)
  14. Han TJ, Kor. J. Environ. Biol., 17, 1 (1999)
  15. Kim YM, Kim MR, Kwon TH, Ha JM, Lee JH, J. Korean Ind. Eng. Chem., 20(3), 285 (2009)
  16. Kim JY, Joo H, Lee JH, Appl. Chem. Eng., 22(3), 301 (2011)
  17. Chen W, Sommerfeld M, Hu QA, Bioresour. Technol., 102(1), 135 (2011)
  18. Lee SI, Park JY, Jung JG, Lee DG, Lee SH, Ha JM, Ha BJ, Lee JH, J. Life Sci., 15, 847 (2005)
  19. Teramura AH, Ziska LH, Sztein AE, Physiol. Plant., 83, 373 (1991)
  20. Singh SC, Sinha RP, Hader DP, Acta Protozool., 41, 297 (2002)
  21. Rosenberg JN, Oyler GA, Wilkinson L, Betenbaugh MJ, Curr. Op. Biotechnol., 19, 430 (2008)
  22. Boussiba S, Physiol Plant., 108, 111 (2000)