닥나무 추출물의 항산화 활성 및 성분 분석

박수아; 하지훈; 박수남; Su Ah Park; Ji Hoon Ha; Soo Nam Park

Applied Chemistry for Engineering, Vol.24, No.2, 177-183, April, 2013

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닥나무 추출물의 항산화 활성 및 성분 분석

Antioxidative Activity and Component Analysis of Broussonetia kazinoki SIEB Extracts

박수아, 하지훈, 박수남 ^†

서울과학기술대학교 정밀화학과 나노바이오화장품연구실, 화장품종합기술연구소

Su Ah Park, Ji Hoon Ha, and Soo Nam Park^†

Department of Fine Chemistry, Nanobiocosmetic Laboratory and Cosmetic R&D Center, Seoul National University of Science and Technology, Seoul 139-743, Korea

E-mail:

초록

본 연구에서는 닥나무 추출물의 항산화 활성과 성분 분석에 관한 연구를 실시하였다. 닥나무 추출물은 우수한 free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) 소거활성(FSC50 = 8.53 μg/mL)을 나타내었다. Luminol-의존성 화학발광법을 이용한 Fe3+-EDTA/H2O2계에서 생성된 활성 산소종(reactive oxygen species, ROS)에 대한 에틸아세테이트 분획의 총항산화능(OSC50)은 1.69 μg/mL이었다. 닥나무 추출물에 대하여 1O2으로 유도된 사람 적혈구의 광용혈 실험 결과 에틸아세테이트 분획은 10 μg/mL의 낮은 농도에서 τ50이 183.3 min으로 대표적인 항산화 물질로 알려진 α-tocopherol (τ50 = 38.00 min) 보다 매우 큰 세포보호 효과를 나타내었다. TLC, HPLC, LC/ESI-MS/MS 및 1H-NMR을 이용하여 닥나무 추출물 에틸아세테이트 분획의 성분 분석을 수행하였다. 그 결과, 닥나무 추출물 에틸아세테이트 분획은 kazinol류의 kazinol J와 플라보노이드류인 luteolin을 함유하고 있음을 확인하였다. 이상의 결과들은 닥나무 추출물이 1O2 혹은 다른 ROS를 소광시키거나 소거함으로써, 또는 이에 대항하여 세포막을 보호함으로써, 생체계 특히 태양 자외선에 노출된 피부에서 항산화제로서 작용할 수 있음을 가리키며 기능성 화장품 원료로서 응용 가능성이 있음을 시사한다.

In this study, the antioxidative activities and component analysis of Broussonetia kazinoki SIEB (B. kazinoki). extracts were investigated. B. kazinoki extract showed the effective free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (FSC50 = 8.53 μg/mL). Reactive oxygen species (ROS) scavenging activity (OSC50) of the ethyl acetate fraction of B. kazinoki. extracts in the luminol-dependent Fe3+-EDTA/H2O2 system was 1.69 μg/mL. The ethyl acetate fraction of B. kazinoki. extracts also exhibited more prominent cellular protective effects (τ50, 183.3 min at 10 μg/mL) than that of typical antioxidant α-tocopherol (τ50 = 38.00 min) in the 1O2-induced photohemolysis of human erythrocytes. Components of the ethyl acetate fraction obtained from B. kazinoki extracts were analyzed by TLC, HPLC chromatogram, LC/ESI-MS/MS and 1H-NMR. Consequently, Components, components were identified as the kazinol J of kazinol series and luteolin (2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4-chromenone) of flavonoid series having antioxidant activities. These results indicate that extract/fraction of B. kazinoki can be used as antioxidants in biological systems, particularly skins exposed to UV radiation by quenching and/or scavenging 1O2 and other ROS, and protecting cellular membranes against ROS. Thus, the extract/fraction of B. kazinoki could be applicable to new cosmeceuticals.

Keywords:B. kazinoki;antioxidant;component analysis;reactive oxygen species

[References]

Lee DS, Lim MS, Kwan SS, Kim SY, Park SN, Appl. Chem. Eng., 23(1), 93 (2012)
Kim AR, Kim JE, Park SN, J. Soc. Cosmet. Scientists Korea., 37, 309 (2011)
Kim JE, Chae KY, Park SN, J. Soc. Cosmet. Scientists Korea., 37, 265 (2011)
Choi CH, Won DH, Hwang JP, Park SN, J. Soc. Cosmet. Scientists Korea., 38, 275 (2012)
Michael JH, Frederick ED, Free Radic. Biol. Med., 43, 1023 (2007)
Magdalena LC, Tak YA, Free Radic. Biol. Med., 48, 749 (2010)
Masaki H, J. Dermatol. Sci., 58, 85 (2010)
Ma W, Wlaschek M, Brenneisen P, Schneider LA, Hommel C, Hellweg C, Sauer H, Wartenberg M, Herrmann G, Meewes C, Boukamp P, Scharffetter-Kochanek K, Exp. Cell. Res., 274, 299 (2002)
Allen RG, Tresini M, Free Radic. Biol. Med., 28, 463 (2000)
Park SN, J. Korean Ind. Eng. Chem., 14(5), 657 (2003)
Park SN, Korean. J. Food Sci. Technol., 35, 510 (2003)
Kim MJ, Yang HG, Park SN, J. Soc. Cosmet. Scientists Korea., 37, 191 (2011)
Shimi zu K, Kondo R, Sakai K, Takeda N, Nagahata T, Planta Med., 68, 79 (2002)
Jukiewicz BA, Bissett DL, Buettner GR, J. Invest. Dermatol., 104, 484 (1995)
Black DL, Chatterjee R, Hannon DP, Photochem. Photobio., 54, 215 (2008)
Fantone JC, Ward PA, Ann. J. Path., 107, 397 (1982)
Hwang BS, Isolation and identification of biological activity compounds from Paper mulbery (Broussonetia kazinoki), Woosuk Univ., Jeollabuk-do, Korea (2009)
Zhang PC, Wang S, Wu Y, Chen RC, Yu DQ, J. Nat. Prod., 64, 1206 (2001)
Baek YS, Tyrosinase inhibitory effects of 1,3-diphenylpropanes from Broussonetia kazinoki, Gyeongsang national university (2009)
Lee HJ, Park JH, Jang DI, Ryu JH, Yakhak Hoeji., 41, 439 (1997)
Ryu JH, Ahn H, Lee HJ, Fitoterapia., 74, 350 (2003)
Kim AY, Lee CG, Lee DY, Li H, Jeon RO, Ryu JH, Kim SG, Free Radic. Biol. Med., 53, 1198 (2012)

[Referenced By]

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[1] Lee DS, Lim MS, Kwan SS, Kim SY, Park SN, Appl. Chem. Eng., 23(1), 93 (2012)

[2] Kim AR, Kim JE, Park SN, J. Soc. Cosmet. Scientists Korea., 37, 309 (2011)

[3] Kim JE, Chae KY, Park SN, J. Soc. Cosmet. Scientists Korea., 37, 265 (2011)

[4] Choi CH, Won DH, Hwang JP, Park SN, J. Soc. Cosmet. Scientists Korea., 38, 275 (2012)

[5] Michael JH, Frederick ED, Free Radic. Biol. Med., 43, 1023 (2007)

[6] Magdalena LC, Tak YA, Free Radic. Biol. Med., 48, 749 (2010)

[7] Masaki H, J. Dermatol. Sci., 58, 85 (2010)

[8] Ma W, Wlaschek M, Brenneisen P, Schneider LA, Hommel C, Hellweg C, Sauer H, Wartenberg M, Herrmann G, Meewes C, Boukamp P, Scharffetter-Kochanek K, Exp. Cell. Res., 274, 299 (2002)

[9] Allen RG, Tresini M, Free Radic. Biol. Med., 28, 463 (2000)

[10] Park SN, J. Korean Ind. Eng. Chem., 14(5), 657 (2003)

[11] Park SN, Korean. J. Food Sci. Technol., 35, 510 (2003)

[12] Kim MJ, Yang HG, Park SN, J. Soc. Cosmet. Scientists Korea., 37, 191 (2011)

[13] Shimi zu K, Kondo R, Sakai K, Takeda N, Nagahata T, Planta Med., 68, 79 (2002)

[14] Jukiewicz BA, Bissett DL, Buettner GR, J. Invest. Dermatol., 104, 484 (1995)

[15] Black DL, Chatterjee R, Hannon DP, Photochem. Photobio., 54, 215 (2008)

[16] Fantone JC, Ward PA, Ann. J. Path., 107, 397 (1982)

[17] Hwang BS, Isolation and identification of biological activity compounds from Paper mulbery (Broussonetia kazinoki), Woosuk Univ., Jeollabuk-do, Korea (2009)

[18] Zhang PC, Wang S, Wu Y, Chen RC, Yu DQ, J. Nat. Prod., 64, 1206 (2001)

[19] Baek YS, Tyrosinase inhibitory effects of 1,3-diphenylpropanes from Broussonetia kazinoki, Gyeongsang national university (2009)

[20] Lee HJ, Park JH, Jang DI, Ryu JH, Yakhak Hoeji., 41, 439 (1997)

[21] Ryu JH, Ahn H, Lee HJ, Fitoterapia., 74, 350 (2003)

[22] Kim AY, Lee CG, Lee DY, Li H, Jeon RO, Ryu JH, Kim SG, Free Radic. Biol. Med., 53, 1198 (2012)