화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.21, No.8, 860-869, August, 2013
DOI10.1007/s13233-013-1105-7  Export Citation
Fabrication and characterization of electrospun gelatin-heparin nanofibers as vascular tissue engineering
Heyun Wang1, Yakai Feng2, 3, †, Zichen Fang2, 4, Ruofang Xiao2, 4, Wenjie Yuan2, 4, and Musammir Khan2, 4
  • 1School of Chemistry and Chemical Engineering, Shihezi University, Beisi Road, Shihezi, 830002, P. R. China
  • 2School of Chemical Engineering and Technology, Tianjin University, Weijin Road 92, Tianjin, 300072, P. R. China
  • 3Joint Laboratory for Bio-materials and Regenerative Medicine, Tianjin University-Helmholtz-Zentrum Geesthacht, Weijin Road 92, Tianjin, 300072, China
  • 4, China
E-mail:
In this paper, heparin was introduced into electrospun gelatin nanofibrous scaffold for assessment as a controlled delivery device in vascular tissue engineering application. Hybrid gelatin-heparin fibers with smooth surfaces and no bead defects were produced from gelatin solutions with 18% w/v in acetic acid aqueous solution. A significant decrease in fiber diameter was observed when the heparin content was increased from 1 to 5 wt%. The properties of composite gelatin-heparin scaffolds were confirmed by Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) measurement. The gelatin-heparin fibrous scaffolds were also cross-linked using 1 wt% glutaraldehyde vapor-phase for 7 days. A sustained release of heparin could be achieved from gelatinheparin scaffolds over 14 days. The results of the biocompatibility in vitro tests carried out using human umbilical vein endothelial cells indicated good cell viability and proliferation on the gelatin-heparin scaffolds. The results demonstrated that the use of electrospun gelatin fibers as heparin carriers could be promising for vascular tissue applications.
Keywords:gelatin;heparin;electrospinning;crosslinking;nanofibers;vascular tissue engineering
  1. Kenawy ER, Fouad AH, Mohamed EN, Raphael MO, Polym. Int., 57, 85 (2008)
  2. Luong-Van E, Grondahl L, Chua KN, Leong KW, Victor N, Cool SM, Biomaterials, 27, 2042 (2006)
  3. Casper CL, Yang WD, Farach-Carson MC, Rabolt JF, Biomacromolecules, 8(4), 1116 (2007)
  4. Chew SY, Wen J, Yim EKF, Leong KW, Biomacromolecules, 6(4), 2017 (2005)
  5. Kenawy ER, Bowlin GL, Mansfield K, Layman J, Simpson DG, Sanders EH, Wnek GE, J. Control. Release, 81, 57 (2002)
  6. Kuijpers AJ, Engbers GHM, Meyvis TKL, de Smedt SSC, Demeester J, Krijgsveld J, Zaat SAJ, Dankert J, Feijen J, Macromolecules, 33(10), 3705 (2000)
  7. Taylor PR, Wolfe MJHN, Tyrrell MR, Mansfield AO, Nicolaides AN, Houston RE, Brit. J. Surg., 77, 1125 (1990)
  8. Cohn WE, Curr. Opin. Cardiol., 19, 589 (2004)
  9. Lee KY, Jeong L, Kang YO, Lee SJ, Park WH, Adv. Drug Deliv. Rev., 61, 1020 (2009)
  10. Jaeger R, Bergshoef MM, Batlle CMI, Holger E, Vancso G, Macromol. Symp., 127, 141 (1998)
  11. Doshi J, Reneker DH, J. Electrostat., 35, 151 (1995)
  12. Li WJ, Laurencin CT, Caterson EJ, Tuan RS, Ko FK, J. Biomed. Mater. Res. A, 60, 613 (2002)
  13. Thomas V, Dean DR, Jose MV, Mathew B, Chowdhury S, Vohra YK, Biomacromolecules, 8(2), 631 (2007)
  14. Thomas V, Zhang X, Vohra YK, Biotechnol. Bioeng., 104(5), 1025 (2009)
  15. Wang H, Feng Y, Zhao H, Xiao H, Lu J, Zhang L, Guo J, Macromol. Res., 20(4), 347 (2012)
  16. Kidoaki S, Kwon IK, Matsuda T, Biomaterials, 26, 37 (2005)
  17. Akin H, Hasirci N, J. Appl. Polym. Sci., 58(1), 95 (1995)
  18. Li XK, Cai SX, Liu B, Xu ZL, Dai XZ, Ma KW, Colloids Surf. B: Biointerfaces, 57, 198 (2007)
  19. Chong EJ, Phan TT, Lim IJ, Zhang YZ, Bay BH, Ramakrishna S, Lim CT, Acta Biomater., 3, 321 (2007)
  20. Choi YS, Hong SR, Lee YM, Song KW, Park MH, Nam YS, Biomaterials, 20, 409 (2009)
  21. Li Y, Chen XG, Liu N, Liu CS, Liu CG, Meng XH, Carbohydr. Polym., 67, 227 (2007)
  22. Smith PK, Mallia AK, Hermanson GT, Anal. Biochem., 109, 466 (1980)
  23. Zhang YZ, Venugopal J, Huang ZM, Lim CT, Ramakrishna S, Polymer, 47(8), 2911 (2006)
  24. Huang ZM, Zhang YZ, Ramakrishna S, Lim CT, Polymer, 45(15), 5361 (2004)
  25. Nandana B, Kundu SC, Carbohydr. Polym., 85, 325 (2011)
  26. Song JH, Kim HE, Kim HW, J. Mater. Sci. -Mater. Med., 19, 95 (2008)
  27. Gautam J, Schott H, J. Pharm. Sci., 83, 316 (1994)
  28. Schiffman JD, Schauer CL, Biomacromolecules, 8(2), 594 (2007)
  29. Ko JH, Yin H, An J, Chung DJ, Kim JH, Lee SB, Pyun DG, Macromol. Res., 18(2), 137 (2010)
  30. Han B, Jaurequi J, Tang BW, Nimni ME, J. Biomed. Mater. Res. A, 65, 118 (2003)
  31. Panzavolta S, Gioffre M, Focarete ML, Gualandi C, Foroni L, Bigi A, Acta Biomater., 7, 1702 (2011)
  32. Chen HC, Jao WC, Yang MC, Polym. Adv. Technol., 20, 98 (2009)
  33. Ki CS, Baek DH, Gang KD, Lee KH, Um IC, Park YH, Polymer, 46, 5904 (2005)
  34. Mobarakeh LG, Prabhakaran MP, Morshed M, Nasr-Esfahani MH, Ramakrishna S, Biomaterials, 29, 4532 (2008)
  35. Li MY, Guo Y, Wei Y, Macmiarmid AG, Lelkes PI, Biomaterials, 27, 2705 (2006)
  36. Lee JM, Tae GY, Kim YH, Park IS, Kim SH, Biomaterials, 29, 1872 (2008)
  37. Heo SJ, Kim SE, Wei J, Hyun YT, Yun HS, Kim DH, Shin JW, Shin J, J. Biomed. Mater. Res., 89, 108 (2009)
  38. Wissink MJB, Beernink R, Poot AA, Engbers GHM, Beugeling T, van-Aken WG, Feijen J, J. Control. Release, 64, 103 (2000)
  39. Ishihara M, Sato M, Hattori H, Saito Y, Yura H, Ono K, Masuoka K, Kikuchi M, Fujikawa K, Kurita A, J. Biomed. Mater. Res. A, 56, 536 (2001)
  40. Gospodarowicz D, Cheng J, J. Cell. Physiol., 128, 475 (1986)
  41. Yoon JJ, Chung HJ, Lee HJ, Park TG, J. Biomed. Mater. Res. A, 79, 934 (2006)