Pluronic nanoparticles do not modulate immune responses mounted by macrophages

Hyun Gyung Kim; Sang-Hyun Jo; Seung-min Yeon; Kyong Hoon Kim; Jin Woong Chung; Tae Won Park; Youngjoo Byun; Eun Hee Lee; Young In Park; Yong Woo Jung

Macromolecular Research, Vol.21, No.12, 1355-1359, December, 2013

DOI10.1007/s13233-013-1174-7 Export Citation

Pluronic nanoparticles do not modulate immune responses mounted by macrophages

Hyun Gyung Kim, Sang-Hyun Jo, Seung-min Yeon, Kyong Hoon Kim, Jin Woong Chung¹, Tae Won Park², Youngjoo Byun, Eun Hee Lee, Young In Park, and Yong Woo Jung^†

Department of Pharmacy, Korea University, Sejong, 339-700, Korea
¹Department of Biological Science, Dong-A University, Busan, 604-714, Korea
²Department of Psychiatry, Chonbuk National University Medical School, Jeonbuk, 561-712, Korea

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Nanomaterials have been developed for the target delivery of medicine because they show the characteristics of selective emission or controlled release. Although accumulated data suggest the efficacy of these materials for the target delivery, it still remains to be determined whether they modulate immune responses to pathogens or foreign materials. In this study, we examined whether Pluronic nanoparticles (NPs), a type of nanomaterial, alter immune responses mediated by macrophages. When RAW 264.7 cells (RAW cells) were treated with Pluronic NPs in the presence or absence of lipopolysaccharides (LPS), they produced normal levels of nitric oxide (NO). Furthermore, the treatment with Pluronic nanomaterials did not induce cytotoxicity with or without LPS. Further, LPS-stimulated RAW cells expressed comparable levels of proinflammatory cytokine genes, such as interleukin (IL)-1 β, IL-6 and tumor necrosis factor (TNF)-α, with or without treatment by Pluronic NPs. These data suggest that Pluronic NPs do not modulate immune responses mediated by macrophages.

Keywords:pluronic nanoparticles;lipopolysaccharides;macrophage;nitric oxide;proinflammatory cytokines

[References]

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Ortona O, D'Errico G, Paduano L, Vitagliano V, J. Colloid Interface Sci., 301(1), 63 (2006)
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Akira S, Hirano T, Taga T, Kishimoto T, Faseb J., 4, 2860 (1990)
Kang SS, Ryu YH, Balk JE, Yun CH, Lee K, Chung DK, Han SH, Mol. Immunol., 48, 2170 (2011)
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Geissmann F, Manz MG, Jung S, Sieweke MH, Merad M, Ley K, Science, 327(5966), 656 (2010)
Chellat F, Merhi Y, Moreau A, Yahia L, Biomaterials, 26, 7260 (2005)
Owais M, Gupta CM, Curr. Drug Deliv., 2, 311 (2005)
Shavandi Z, Ghazanfari T, Moghaddam KN, Immunopharmacol. Immunotoxicol., 33, 135 (2011)
Napierska D, Thomassen LC, Rabolli V, Lison D, Gonzalez L, Kisch-Volders M, Martens JA, Hoet PH, Small, 5, 846 (2009)
Waters KM, Masiello LM, Zangar RC, Tarasevich BJ, Karin NJ, Quesenberry RD, Bandyopadhyay S, Teeguarden JG, Pounds JG, Thrall BD, Toxicol. Sci., 107, 553 (2009)
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Kabanov AV, Batrakova EV, Alakhov VY, Abstr. Pap. Am. Chem. Soc., 223, U438 (2002)
Kim JY, Choi WI, Kim YH, Tae G, Lee SY, Kim K, Kwon IC, J. Control. Release, 147, 109 (2010)
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[Referenced By]

[1] Alexandridis P, Holzwarth JF, Hatton TA, Macromolecules, 27(9), 2414 (1994)

[2] Desai PR, Jain NJ, Sharma RK, Bahadur P, Colloids Surf. A: Physicochem. Eng. Asp., 178, 57 (2001)

[3] Ortona O, D'Errico G, Paduano L, Vitagliano V, J. Colloid Interface Sci., 301(1), 63 (2006)

[4] Oh KS, Song JY, Cho SH, Lee BS, Kim SY, Kim K, Jeon H, Kwon IC, Yuk SH, J. Control. Release, 148, 344 (2010)

[5] Yuk SH, Oh KS, Cho SH, Kim SY, Oh S, Lee JH, Kim K, Kwon IC, Mol. Pharm., 9, 230 (2012)

[6] Kabanov AV, Sriadibhatla S, Yang ZH, Alakhov VY, Abstr. Pap. Am. Chem. Soc., 228, U388 (2004)

[7] Kabanov AV, Batrakova EV, Striadibhatrla S, Yang Z, Kelly DL, Alakov VY, J. Control. Release, 101, 259 (2005)

[8] Kabanov AV, Adv. Drug Deliv. Rev., 58, 1597 (2006)

[9] Sriadibhatla S, Yang Z, Gebhart C, Alakhov VY, Kabanov A, Mol. Ther., 13, 804 (2006)

[10] Wang XY, Ishida T, Kiwada H, J. Control. Release, 119, 236 (2007)

[11] Flainik MF, Du Pasquier L, Trends Immunol., 25, 640 (2004)

[12] Areschoug T, Gordon S, Contrib. Microbiol., 15, 45 (2008)

[13] Kawai T, Akira S, Nat. Immunol., 11, 373 (2010)

[14] Takeuchi O, Akira S, Cell, 140, 805 (2010)

[15] MacMicking J, Xie QW, Nathan C, Annu. Rev. Immunol., 15, 323 (1997)

[16] Bosca L, Zeini M, Traves PG, Hortelano S, Toxicology, 208, 249 (2005)

[17] Akira S, Hirano T, Taga T, Kishimoto T, Faseb J., 4, 2860 (1990)

[18] Kang SS, Ryu YH, Balk JE, Yun CH, Lee K, Chung DK, Han SH, Mol. Immunol., 48, 2170 (2011)

[19] Jung YW, Rutishauser RL, Joshi NS, Haberman AM, Kaech SM, J. Immunol., 185, 5315 (2010)

[20] Gu W, Kim KA, Kim DH, Biol. Pharm. Bull., 36, 102 (2013)

[21] Xie J, Lee S, Chen X, Adv. Drug Deliv. Rev., 62, 1064 (2010)

[22] Geissmann F, Manz MG, Jung S, Sieweke MH, Merad M, Ley K, Science, 327(5966), 656 (2010)

[23] Chellat F, Merhi Y, Moreau A, Yahia L, Biomaterials, 26, 7260 (2005)

[24] Owais M, Gupta CM, Curr. Drug Deliv., 2, 311 (2005)

[25] Shavandi Z, Ghazanfari T, Moghaddam KN, Immunopharmacol. Immunotoxicol., 33, 135 (2011)

[26] Napierska D, Thomassen LC, Rabolli V, Lison D, Gonzalez L, Kisch-Volders M, Martens JA, Hoet PH, Small, 5, 846 (2009)

[27] Waters KM, Masiello LM, Zangar RC, Tarasevich BJ, Karin NJ, Quesenberry RD, Bandyopadhyay S, Teeguarden JG, Pounds JG, Thrall BD, Toxicol. Sci., 107, 553 (2009)

[28] Thomassen LCJ, Aerts A, Rabolli V, Lison D, Gonzalez L, Kirsch-Volders M, Napierska D, Hoet PH, Kirschhock CEA, Martens JA, Langmuir, 26(1), 328 (2010)

[29] Kabanov AV, Batrakova EV, Alakhov VY, Abstr. Pap. Am. Chem. Soc., 223, U438 (2002)

[30] Kim JY, Choi WI, Kim YH, Tae G, Lee SY, Kim K, Kwon IC, J. Control. Release, 147, 109 (2010)

[31] Horie M, Stowe M, Tabei M, Kato H, Nakamura A, Endoh S, Morimoto Y, Fujita K, Toxicol. Mech. Methods, doi: 10.3109/15376516.2012.755595 (2013).