포도당과 맥아당 농도 변화에 따른 Bacillus amyloliquefaciens 세포 성장과 a-Amylase 생합성에 관한 연구

차월석; 김종균; 신주연

Journal of the Korean Industrial and Engineering Chemistry, Vol.6, No.6, 1083-1091, December, 1995

Effects of Concentrations of Glucose and Maltose on the Production of α-Amylase and Growth of Bacillus amyloliquefaciens

차월석, 김종균, 신주연

초록

탄소원 농도변화에 의한 회분식 배양시 Bacillus amyloliquefaciens의 성장과 α-amylase 생합성에 대해 조사하였다. 탄소원인 maltose와 glucose는 배양 개시후 8시간 전후부터 12시간 전후에서 탄소원 분해가 거의 다 진행되었으며 세포성장도 최대가 되었다. 건조 최대세포 농도는 maltose 초기 농도가 10g/ℓ일 때 2.40g/ℓ에서 129.03unit/ml로서 탄소원 중 가장 높았다. 그리고 α-amylase 생성에 미친 탄소원 농도는 같은 탄소원에서 그 초기 농도가 높을 때 가장 높은 값의 효소가 생성되었다. 최대 비세포성장 속도는 maltose 초기농도가 10g/ℓ일 때 0.5g/g-hr로서 가장 높게 나타났으며 α-amylase의 최대 비생성속도는 maltose 초기농도 20g/ℓ일 때 26.20unit/mg-hr로 가장 높았으며 전체적으로는 maltose가 가장 높은 값을 나타내고 있다. 부산물의 생성은 lactic acid, acetic acid, ethanol, propionic acid 순이었다.

Cell growth and production of α-amylase and by-products were investigated by Bacillus amyloliquefaciens in batch culture with various carbon sources. All glucose and maltose were consumed as a carbon sources within about 8 to 12hours during cell culture. Maximum dry cell density was obtained with the highest value of 2.40g/ℓ at the initial maltose concentraction of 20g/ℓ. Among the various carbon sources, maximum α-amylase production was obtained with 129.03 uint/ml at the initial maltose concentraction of 10g/ℓ. With higher concentration of carbon sources, the highest activity of α-amylase was obtained. At the initial maltose concentration of 10g/ℓ, maximum specific growth rate, 0.5g/g-hr was observed. With initial maltose concentration of 20g/ℓ, maximum specific α-amylase production rate of 26.20 unit/mg-hr was obtained. As maltose or glucose was used as carbon source, lactic acid and acetic acid were major by-products, while propionic acid and methanol formation were not shown significantly.

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[30] Tsuchiya K, Shinjo A, Shinoyama K, Okasaki M, Mihara Y, J. Ferment. Technol., 45, 497 (1976)