Anaerobic digestion is an attractive process for generation of hydrogen and methane, which involves complex microbial processes on decomposition of organic wastes and subsequent conversion of metabolic intermediates to hydrogen and methane. Comparative performance of a sequential hydrogen and methane fermentation in two stage process and methane fermentation in one stage process were tested in batch reactor at varying ratios of feedstock to microbial inoculum (F/M) under mesophilic incubation. F/M ratios influence biogas yield, production rate, and potential. The highest H-2 and CH4 yields of 55 and 94 mL g(-1) VS were achieved at F/M of 7.5 in two stage process, while the highest CH4 yield of 82 mL g(-1) VS in one stage process was observed at the same F/M. Acetic and butyric acids are the main volatile fatty acids (VFAs) produced in the hydrogen fermentation stage with the concentration range 10-25 mmol L-1. Little concentrations of VFAs were accumulated in methane fermentation in both stage processes. Total energy recovery in two stage process is higher than that in one stage by 18%. This work demonstrated two stage fermentation achieved a better performance than one stage process. Copyright (C) 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.