양극 산화에 의해 티타늄 표면에 형성된 산화 피막이 세포 부착 및 성장에 미치는 영향

노세라; 이용렬; 송호준; 박영준; Se-Ra Noh; Yong-Ryeol Lee; Ho-Jun Song; Yeong-Joon Park

Korean Journal of Materials Research, Vol.16, No.10, 606-613, October, 2006

DOI10.3740/MRSK.2006.16.10.606 Export Citation

양극 산화에 의해 티타늄 표면에 형성된 산화 피막이 세포 부착 및 성장에 미치는 영향

Influence of Anodic Oxidation Film Formed on Titanium onto Cell Attachment and Proliferation

노세라, 이용렬, 송호준, 박영준^†

전남대학교 치의학전문대학원 치과재료학교실 및 치과재료연구소

Se-Ra Noh, Yong-Ryeol Lee, Ho-Jun Song, and Yeong-Joon Park^†

Dept. of Dental Materials and Dental Materials Research Institute, School of Dentistry, Chonnam National University, Gwangju 500-757, Korea

E-mail:

This study was purposed to evaluate the influence of anodically oxidized film on titanium (Ti) onto MG-63 osteoblast-like cell attachment and activity. Only scratch lines created by polishing were seen in ASR and ANO-1 groups. About -thick homogeneous oxide film which has pores of about diameter were formed in ANO-12. The crystalline structure of the oxide films formed by anodization in phosphoric acid electrolyte was . The total protein amounts of ANO-1 and ANO-12 groups showed higher values of maximum protein amount than that of AS-R group. At 3 days of incubation, total protein amount showed higher value in ANO-2 when comparing to that of AS-R (p<0.05). Based on the results of ALPase activity test, the degree of MG-63 cell differentiation for initial mineralization matrix formation was similar. For all the test groups after 1 day of incubation, MG-63 cells grew healthily in mono-layer with dendritic extensions. After incubation for 3 days, the specimen surfaces were covered more densely by cells, and numerous micro filaments were extruding to the extracellular matrix.

Keywords:anodization;titanium oxide;implant;osteoblast-like cell;ALPase activity

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[1] Sul YT, Johansson CB, Petronis S, Krozer A, Jeong YS, Wennergerg A, Albrektsson T, Biomaterials, 23, 491 (2002)

[2] Fini M, Cigada A, Rondelli G, Chiesa R, Giardino R, Giavaresi G, Aldini NN, Torricelli P, Vicentini B, Biomaterials, 20, 1587 (1999)

[3] Frauchiger VM, Schlottig F, Gasser B, Textor M, Biomaterials, 25, 593 (2004)

[4] Schreckenbach JP, Marx G, Schlottig F, Textor M, Spencer ND, J. Mater. Sci. Mater. Med., 10, 453 (1999)

[5] Montanaro L, Arciola CR, Campoccia D, Cervellati M, Biomaterials, 23, 3651 (2002)

[6] Rodriguez R, Kim KH, Org JL, J. Biomed. Mater. Res., 65A, 352 (2003)

[7] Son WW, Zhu X, Shin HI, Ong JL, Kim KH, J. Biomed. Mater. Res. Part B: Appl. Biomater., 66B, 520 (2003)

[8] Zhu X, Ong JL, Kim SY, Kim KH, J. Biomed. Mater. Res., 60, 333 (2002)

[9] Son WW, Kim KH, Kim HI, Hanawa T, Jeong YS, Biomater. Res., 4, 66 (2000)

[10] Hanawa T, Ota M, Biomaterials, 12, 767 (1991)

[11] Sul YT, Johnsson CB, Jeong YS, Albrektsson T, Medical Engineering & Physics, 23, 329 (2001)

[12] Wiedmamm-Al-Ahmad M, Gutwald R, Lauer G, Hbner U, Schmelzeisen R, Biomaterials, 23, 3319 (2002)

[13] Martin IY, Schwartz Z, Hummert TW, Schraub DM, Lankford J, Dean JD, Cochran DL, Boyan BD, J. Biomed. Mater. Res., 29, 389 (1995)

[14] Ong JL, Hoppe CA, Cardenas HL, Cavin R, Carnes DL, Sogal A, Raikar GN, J. Biomed. Mater. Res., 39, 176 (1998)

[15] Amaral M, Costa MA, Lopes MA, Silva RF, Santos JD, Fernandes MH, Biomaterials, 23, 4897 (2002)

[16] Evridiki P, Mirsini K, Irenc VB, Christos K, Evangelos M, Biol. Cell, 94, 117 (2002)

[17] Krizmanich WJ, Lee RMKW, Exp. Mol. Pathol., 64, 157 (1997)

[18] Clinton JD, Biological techniques for transmission and scanning electron microscopy, p.231, Ladd research industries, Florida. (1971)

[19] Joseph IG, Dale EN, Partick E, David CJ, Romig JAD, Charles EL, Charles F, Eric L, Scanning electron microscopy and x-ray microanalysis, p.582, Plenum press, New York, (1992) (1992)

[20] Ten Cate A, Oral histology, 4th ed., p.111, Mosby-year book, St. Louis, (1994) (1994)

[21] Okazaki K, Ceramic engineering for dielectrics, p.171, Gakken-sha publishing Co., Tokyo, (1992) (1992)

[22] Reinholz GG, Getz B, Pederson L, Sanders ES, Subramaniam M, Ingle JN, Spelsberg TC, Cancer Res., 60, 6001 (2000)

[23] Suh JY, Jang BC, Zhu X, Ong JL, Kim KH, Biomaterials, 24, 347 (2003)

[24] Larsson C, Thomsen P, Aronsson BO, Rondahl M, Lausmaa J, Kasemo B, Ericson LE, Biomaterials, 17, 605 (1996)

[25] Cooper LF, Masuda T, Whitson SW, Yliheikkil P, David A, Int. J. Oral Maxillofac. Implants., 14, 37 (1999)