Injectable in situ forming chitosan-based hydrogels for curcumin delivery

Titima Songkroh; Hongguo Xie; Weiting Yu; Xiudong Liu; Guangwei Sun; Xiaoxi Xu; Xiaojun Ma

Macromolecular Research, Vol.23, No.1, 53-59, January, 2015

DOI10.1007/s13233-015-3006-4 Export Citation

Injectable in situ forming chitosan-based hydrogels for curcumin delivery

Titima Songkroh^{1, 2}, Hongguo Xie^{1, 3}, Weiting Yu^{1, 3}, Xiudong Liu⁴, Guangwei Sun^{1, 3}, Xiaoxi Xu^{1, 3}, and Xiaojun Ma^{1, 3, †}

¹Dalian Institute of Chemical Physics (DICP), Chinese Academy of Sciences (CAS), 457 Zhongshan Road, Dalian, Liaoning, 116023, P. R. China
²University of Chinese Academy of Sciences (UCAS), 19A Yuquan Road, Beijing, 100049, P. R. China
³, China
⁴College of Environment and Chemical Engineering, Dalian University, 10 Xuefu Street, Economic and technical development zone, Dalian, Liaoning, 116622, P. R. China

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In this paper, a series of injectable in situ forming chitosan-based hydrogels were prepared by chemical crosslinking of chitosan and genipin with the cooperation of ionic bonds between chitosan and sodium salts at room temperature. Four hydrogels (A, B, C, and D) were obtained by mixing chitosan, genipin and a sodium salt of trisodium phosphate (Na3PO4·12H2O), sodium sulfate (Na2SO4), sodium sulfite (Na2SO3), or sodium bicarbonate (NaHCO3) and examined for their characteristics, morphology, and rheological properties. Their cell viability assays exhibited low toxicity and the localized in situ gel formation was detected after subcutaneous injections in rat. Curcumin which possesses many pharmaceutical potentials but has low bioavailability, was chosen as a drug model. In vitro curcumin release profiles exhibit sustained release properties with initial burst release for all hydrogels with about 3 to 6 times higher cumulative release than other gel controls. The results of this study demonstrate that our hydrogels have a potential as local curcumin carriers.

Keywords:chitosan;hydrogel;curcumin;drug delivery

[References]

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[Referenced By]

[1] Annabi N, Tamayol A, Uquillas JA, Akbari M, Bertassoni LE, Cha C, Camci-Unal G, Dokmeci MR, Peppas NA, Khademhosseini A, Adv. Mater., 26(1), 85 (2014)

[2] Croisier F, Jerome C, Eur. Polym. J., 49, 780 (2013)

[3] Hoffman AS, Adv. Drug Deliv. Rev., 64, 18 (2012)

[4] Wolinsky JB, Colson YL, Grinstaff MW, J. Control. Release, 159, 14 (2012)

[5] Bhattarai N, Gunn J, Zhang M, Adv. Drug Deliv. Rev., 62, 83 (2010)

[6] Drury JL, Mooney DJ, Biomaterials, 24, 4337 (2003)

[7] Peppas NA, Bures P, Leobandung W, Ichikawa H, Eur. J. Pharm. Biopharm., 50, 27 (2000)

[8] Hennink WE, van Nostrum CF, Adv. Drug Deliv. Rev., 54, 13 (2002)

[9] Speer DP, Chvapil M, Eskelson CD, Ulreich J, J. Biomed. Mater. Res. A, 14, 753 (1980)

[10] Chenite A, Chaput C, Wang D, Combes C, Buschmann MD, Hoemann CD, Leroux JC, Atkinson BL, Binette F, Selmani A, Biomaterials, 21, 2155 (2000)

[11] Ruel-Gariepy E, Chenite A, Chaput C, Guirguis S, Leroux JC, Int. J. Pharm., 203, 89 (2000)

[12] Moura MJ, Faneca H, Lima MP, Gil MH, Figueiredo MM, Biomacromolecules, 12(9), 3275 (2011)

[13] Muzzarelli RAA, Carbohydr. Polym., 77, 1 (2009)

[14] Wilczewska AZ, Niemirowicz K, Markiewicz KH, Car H, Pharmacol. Rep., 64, 1020 1 (2012)

[15] Tiwari G, Tiwari R, Sriwastawa B, Bhati L, Pandey S, Pandey P, Bannerjee SK, Int. J. Pharm. Investig., 2, 2 (2012)

[16] Aggarwal BB, Harikumar KB, Int. J. Biochem. Cell Biol., 41, 40 (2009)

[17] Anand P, Thomas SG, Kunnumakkara AB, Sundaram C, Harikumar KB, Sung B, Tharakan ST, Misra K, Priyadarsini IK, Rajasekharan KN, Aggarwal BB, Biochem. Pharmacol., 76, 1590 (2008)

[18] Johnson JJ, Mukhtar H, Cancer Lett., 255, 170 (2007)

[19] Maheshwari RK, Singh AK, Gaddipati J, Srimal RC, Life Sci., 78, 2081 (2006)

[20] Anand P, Kunnumakkara AB, Newman RA, Aggarwal BB, Mol. Pharm., 4, 807 (2007)

[21] Sowasod N, Nakagawa K, Tanthapanichakoon W, Charinpanitkul T, Mater. Sci. Eng. C, 32, 790 (2012)

[22] Li X, Chen S, Zhang B, Li M, Diao K, Zhang Z, Li J, Xu Y, Wang X, Chen H, Int. J. Pharm., 437, 110 (2012)

[23] Sun M, Su X, Ding B, He X, Liu X, Yu A, Lou H, Zhai G, Nanomedicine-UK, 7, 1085 (2012)

[24] Anitha A, Maya S, Deepa N, Chennazhi KP, Nair SV, Tamura H, Jayakumar R, Carbohydr. Polym., 83, 452 (2011)

[25] Ma X, Songkroh T, Yu W, Xie H, Liu X, Xie W, PCT Patent WO2014005471A1 (2014)

[26] Ma X, Xie H, Yu W, Songkroh T, Chinese Patent CN103524795A (2014)

[27] Wang MO, Etheridge JM, Thompson JA, Vorwald CE, Dean D, Fisher JP, Biomacromolecules, 14(5), 1321 (2013)

[28] Lavertu M, Filion D, Buschmann MD, Biomacromolecules, 9(2), 640 (2008)

[29] Mi FL, Shyu SS, Peng CK, J. Polym. Sci. A: Polym. Chem., 43(10), 1985 (2005)

[30] Mi FL, Sung HW, Shyu SS, J. Polym. Sci. A: Polym. Chem., 38(15), 2804 (2000)

[31] Woodard G, in Methods of Animal Experimentation, Gay WJ, Ed., Academic Press, New York, Vol. 1, p 343. (1965)

[32] Shimizu S, in Chapter 32 - Routes of Administration, Hedrich HJ, Bullock G, Eds., Academic Press, New York, p 527. (2004)

[33] Ishiyama M, Miyazono Y, Shiga M, Sasamoto K, US Patent US6063587A (2000)

[34] Riva R, Ragelle H, des Rieux A, Duhem N, Jerome C, Preat V, Adv. Polym. Sci., 244, 19 (2011)

[35] Kumar MNVR, Muzzarelli RAA, Muzzarelli C, Sashiwa H, Domb AJ, Chem. Rev., 104(12), 6017 (2004)

[36] Paik YS, Lee CM, Cho MH, Hahn TR, J. Agric. Food Chem., 49, 430 (2001)

[37] Brewer CP, Dawson B, Wallman KE, Guelfi KJ, Int. J. Sport Nutr. Exerc. Metab., 23, 187 (2013)

[38] Lukaski HC, in Chapter 10 - Magnesium, Phosphate, and Calcium Supplementation and Human Physical Performance, Driskell JA, Wolinsky I, Eds., CRC Press, Florida, p 197. (2004)

[39] Regulation (EC) No 1333/2008 (OJ L354, p16, 31/12/2008) of the European Parliament and of the Council of 16 December 2008 on Food Additives (2013)