Journal of Fermentation and Bioengineering, Vol.86, No.3, 332-334, 1998
Molecular cloning and expression of a polygalacturonase gene in Saccharomyces cerevisiae
An endo-polygalacturonase gene was cloned from a mutant of Saccharomyces cerevisiae that can produce a polygalacturonase, and the nucleotide sequence was identified. The polygalacturonase gene consists of 1086 bp. When the polygalacturonase gene was introduced into a strain of S. cerevisiae that cannot produce a polygalacturonase, the transformant secreted a polygalacturonase identical to that produced by the polygalacturonase-producing mutant.