Aeromonas sobria LP004 harboring the Acinetobacter calcoaceticus lipase gene was designated as strain LP094. Lipase production of LP094 in WYGS medium [Lotrakul and Dharmsthiti, World J. Microbiol. Biotechnol., 13:163-166, 1997] was scaled up to a 50-l volume. LP094 lipase immobilized by ionic binding to either IR120 (Na+) or IRC50 (H+) Amberlite resins was found to retain more than 90% activity after 15 d storage at room temperature (approximate to 25 to 30 degrees C) or at 4 degrees C. After 5 repeated lipid hydrolysis reactions, the activities of both immobilized preparations remained higher than 65%.