화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.27, 25-30, July, 2015
DOI10.1016/j.jiec.2014.12.022  Export Citation
Response surface methodology based optimization of keratinase production from alkali-treated feather waste and horn waste using Bacillus sp. MG-MASC-BT
Muthusamy Govarthanan1, 2, †, Thangasamy Selvankumar1, 3, Kandasamy Selvam4, Chinnappan Sudhakar1, 3, Vincent Aroulmoji1, 3, and Seralathan Kamala-Kannan2, †
  • 1PG & Research Department of Biotechnology, Mahendra Arts and Science College, Kalippatti, Namakkal 637501, Tamil Nadu, India
  • 2Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570752, South Korea
  • 3, India
  • 4Centre for Biotechnology, Muthayammal College of Arts and Science, Rasipuram, Namakkal 637 408, Tamil Nadu, India, Korea
E-mail:,
A native feather-degrading keratinolytic bacterium, Bacillus sp. MG-MASC-BT was isolated from feather dumping soil in Mallasamudram, Tamil Nadu, India and screened for keratinase production using alkali treated horn waste (HW) and feather waste (FW). The study factors influencing keratinase production was optimized by Box?Behnken design (BBD). The maximum enzyme production (1075 U/ml) was observed at pH 7.0, temperature 55 °C and growth period of 60 h. The media supplemented with 6% of HW and FW enhanced keratinase production. Statistics based contour plots were generated to evaluate the changes in the response surface and to understand the relationship between the enzyme yield and the culture conditions.
Keywords:Feather;Horn;Keratinase;Solid state fermentation;Bacillus sp.
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