Kinetic study of acetaldehyde conversion to ethanol by free and CNT-immobilized baker’s yeast in a gas-phase packed bed reactor

Khadijeh Sherafatmand; Zeinab Salehi; Shohreh Fatemi

Journal of Industrial and Engineering Chemistry, Vol.30, 160-166, October, 2015

DOI10.1016/j.jiec.2015.05.017 Export Citation

Kinetic study of acetaldehyde conversion to ethanol by free and CNT-immobilized baker’s yeast in a gas-phase packed bed reactor

Khadijeh Sherafatmand, Zeinab Salehi, and Shohreh Fatemi^†

School of Chemical Engineering, College of Engineering, University of Tehran, Enghlab Ave., Tehran 123451, Iran

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Catalytic activity of the whole cell baker’s yeast (Saccharomyces cerevisiae) was studied for converting acetaldehyde (AC) to ethanol (ET) in a gas-phase packed bed reactor. The baker’s yeast was considered at free and immobilized conditions, to convert AC as an air pollutant to the less harmful product of ET. The influence of operating conditions, such as inlet AC concentration, cells’ water content and temperature, was studied on the cells’ activity. The best conditions from the point of ET productivity and cell stability was detected at temperature of 318 K and water content of 0.23 ± 0.02 g water/g dry cell, using free cells. The cell immobilization was performed by doping on the surface of multiwalled carbon nanotube (CNT) under a mild centrifugal force. The activity of CNT-immobilized cells towards ET formation was improved by 6% and the stability of the cells was improved by 43% respectively in comparison with the free cells’ activity and stability.

Keywords:Baker’s yeast;Free cells;CNT-immobilized cells;Biocatalyst;Acetaldehyde conversion;ethanol;packed;bed reactor

[References]

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[1] U.S. Environmental Protection Agency, EPA/600/8-86-015A, Environmental Criteria and Assessment Office, Office of Research and Development, Research Triangle Park, NC, 1987 (Revised in January 2000).

[2] Sittig M, Handbook of Toxic and Hazardous Chemicals and Carcinogens, second ed., Noyes Publications, Park Ridge, NJ, 1985.

[3] Csuk R, Glanzer BI, Chem. Rev., 9, 49 (1991)

[4] Ghaedi M, Hajati S, Barazesh B, Karimi F, Ghezelbash G, J. Ind. Eng. Chem., 19(1), 227 (2013)

[5] Shahirah MNN, Gimbun J, Pang SF, Zakria RM, Cheng CK, Chua GK, Asras MFF, J. Ind. Eng. Chem., 23, 213 (2015)

[6] Presecki AV, Vasic-Racki D, Process Biochem., 40, 2781 (2005)

[7] Lamare S, Legoy MD, Biocatalysis in the Gas Phases, Elsevier Science Publishers Ltd (UK), France, 1993.

[8] Kimura K, Suzuki A, Inokuchi H, Yagi T, Biochim. Biophy. Acta, 567, 96 (1979)

[9] Sheldon BJ, Duff WD, Biotechnol. Bioeng., 34, 153 (2004)

[10] Pulvin S, Legoy MD, Lortie R, Pensa M, Thomas D, Biotechnol. Lett., 8, 783 (1986)

[11] Erable B, Maugard T, Goubet I, Lamare S, Legoy MD, Process Biochem., 40, 45 (2005)

[12] Chui-Ho K, Sang-Ki R, Biotechnol. Lett., 14, 105 (1992)

[13] Maugard T, Lamare S, Legoy MD, Biotechnol. Bioeng., 73(2), 164 (2001)

[14] Tan A, Yildirimer L, Rajadas J, De La Pena H, Pastorin G, Seifalian A, Nano Med., 6, 1101 (2011)

[15] Tourani S, Khorasheh F, Rashidi AM, Safekordi AA, J. Ind. Eng. Chem., 28, 202 (2015)

[16] Ago H, Uchimura E, Saito T, Ohshima S, Ishigami N, Tsuji M, Yumura M, Miyake M, Mater. Lett., 60, 3851 (2006)

[17] Simate GS, J. Ind. Eng. Chem., 21, 1277 (2015)

[18] Jung C, Son A, Her N, Zoh KJ, Cho J, Yoon Y, J. Ind. Eng. Chem., 27, 1 (2015)

[19] Sucapane A, Cellot G, Prato M, Giugliano M, Parpura V, Ballerini L, Nano Neurosci., 1, 10 (2009)

[20] Matsumoto K, Sato C, Naka Y, Kitazawa A, Whitby RLD, Shimizu N, J. Biosci. Bioeng., 103(3), 216 (2007)

[21] Mattson MP, Haddon RC, Rao AM, Mol. Neurosci., 14, 175 (2000)

[22] Guisan JM, Methods in Biotechnology, Human Press, New Jersey, 2006.

[23] Folch A, Toner M, Ann. Rev. Biomed Eng., 2, 227 (2000)

[24] Paquette LA, Chiral Reagents for Asymmetric Synthesis, Wiley, UK, 2003.

[25] Jayasinghe LY, Smallridge AJ, Trewhella MA, Tetrahedron Lett., 34, 3949 (1993)

[26] Fow KL, Synthesis of Optically Active Secondary Alcohols by Chemocatalysis and Biocatalysis, Singapore National University, Singapore, 2010 (Ph.D. Thesis).

[27] Salic A, Faletar P, Zelic B, Biochem. Eng. J., 77, 88 (2013)

[28] Alves G, Filho B, Lobato A, Tan D, Neto C, Costa R, Avila F, Marques D, Galate R, Plant Omics J., 5, 216 (2012)

[29] Belenky P, Katrina BL, Brenner C, Trends Biochem. Sci., 32, 1 (2006)

[30] Fow KL, Poon L, Sim S, Chuah G, Jaenicke S, Eng. Life Sci., 8, 372 (2008)

[31] Dickinson FM, Monger GP, Biochem. J., 131, 261 (1973)