Highly efficient gold nanorods assisted laser phototherapy for rapid treatment on mice wound infected by pathogenic bacteria

M. Shahnawaz Khan; Mukesh L. Bhaisare; Judy Gopal; Hui-Fen Wu

Journal of Industrial and Engineering Chemistry, Vol.36, 49-58, April, 2016

DOI10.1016/j.jiec.2015.12.011 Export Citation

Highly efficient gold nanorods assisted laser phototherapy for rapid treatment on mice wound infected by pathogenic bacteria

M. Shahnawaz Khan¹, Mukesh L. Bhaisare², Judy Gopal^{2, 3}, and Hui-Fen Wu^{2, 4, †}

¹Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University and Academia Sinica, Kaohsiung 80424, Taiwan
²Department of Chemistry and Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, Kaohsiung 80424, Taiwan
³Department of Molecular Biotechnology, Konkuk University, Seoul 143-701, Korea
⁴School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan

E-mail:

Treatment of wound infection is one of the most challenging problems to be addressed in infectiously microbiological world. This is mainly due to the pathogen's ability for fast mutation and generating severely antibiotic resistance to antimicrobial treatment. Pathogens causing wound infection treated by photothermal methods can avoid drug resistance problem. Beside, rapid and highly effective wound treatment method in modern medical technology is extremely required. Therefore, we have proposed a novel method by using gold nanorods (Au NRs) to assist the Nd-YAG laser (1064 nm) for photothermal killing pathogenic bacteria (Pseudomonas aeruginosa) for directly healing the wound infection on the (albino) mice. In all experiments, the bacteria numbers were calculated by the plate count method and we also used MALDI-MS to evaluate the effectiveness of the treatment and the physiological condition of wound infection. The current approach can be used to control severe skin infections from antibiotic resistant pathogens in wounds.

Keywords:MALDI-MS;Mice wound healing;Pseudomonas aeruginosa;Nd-YAG laser

[References]

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Lyons RF, Abergel RP, White RA, Dwyer RM, Costel JC, J. Ann. Plast. Surg., 1847 (1987)
Kesava RG, Lasers Surg. Med., 33, 344 (2003)

[1] Diegelmann RF, Evans MC, Front. Biosci., 9, 283 (2004)

[2] Robson MC, Mustoe TA, Hunt TK, Am. J. Surg., 176, 80 (1998)

[3] Makowski AJ, Jansen ED, Davidson JM, Jansen AM, Lasers Surg. Med., 44, 233 (2012)

[4] Medrado AR, Pugliese LS, Reis SR, Andrade ZA, Lasers Surg. Med., 32, 239 (2003)

[5] Festini F, Buzzetti R, Bassi C, Braggio C, Salvatore D, Taccetti G, Mastella G, J. Hosp. Infect., 64, 1 (2006)

[6] Lyczak JB, Cannon CL, Pier GB, Microbes Infect., 2, 1051 (2006)

[7] Norman RS, Stone JW, Gole A, Murphy CJ, Sabo-Attwood TL, Nano Lett., 8, 302 (2008)

[8] Galdiero S, Falanga A, Vitiello M, Cantisani M, Marra V, Galdiero M, Molecules, 16, 8894 (2011)

[9] Ratto F, Matteini P, Tiwari N, Rossi F, Kulkarni SK, Menabuoni L, Pini R, Nanomed.: Nanotechnol. Biol. Med., 5, 143 (2009)

[10] Yasun E, Gulbakan B, Ocsoy I, Yuan Q, Shukoor MI, Li C, Tan W, Am. Chem. Soc., 14, 6008 (2012)

[11] Palanisamy NK, Ferina N, Amirulhusni AN, Zain ZM, Ping LJ, Durairaj R, Hussaini J, J. Nanobiotechnol., 12, 2 (2014)

[12] Gobin AM, West LL, Patrick ON, Watkins DM, Halas NJ, Drezek RA, Lasers Surg. Med., 37, 123 (2005)

[13] Joshi PP, Joon YSJ, Sokolov KV, Hardin WG, Emelianov S, Bioconjugate Chem., 24, 878 (2013)

[14] Zhang Q, Hitchins VM, Schrand AM, Hussain SM, Goering PL, Nanotoxicology, 55, 284 (2011)

[15] Gui C, Cui DX, Cancer Biol. Med., 9, 221 (2012)

[16] Huang XH, El-Sayed IH, Qian W, El-Sayed MA, J. Am. Chem. Soc., 128(6), 2115 (2006)

[17] Loo C, Lowery A, Halas N, West J, Drezek R, Nano Lett., 5, 709 (2005)

[18] Maltzahn GV, Park JH, Lin KY, Singh N, Mesters CSR, Berdel WE, Ruoslahti E, Sailor MJ, Bhatia SN, Nat. Mater., 109, 545 (2011)

[19] Dadpay M, Sharifian Z, Bayat B, Bayat M, Dabbagh A, J. Photochem. Photobiol. B-Biol., 111, 1 (2012)

[20] Al-Watban FA, Zhang XY, J Clin. Laser Med. Surg., 22, 15 (2004)

[21] Hashmi JT, Kurup B, Huang YY, Sharma SK, Taboada DLD, Carroll JD, Hamblin MR, Lasers Surg. Med., 42, 450 (2010)

[22] Zolfaghari PS, Packer S, Singer M, Nair SP, Bennett J, Street C, Wilson M, BMC Microbiol., 9, 27 (2009)

[23] Kojic N, Pritchard EM, Tao H, Brenckle MA, Mondia JP, Panilaitis P, Omenetto F, Kaplan DL, Adv. Funct. Mater., 12, 3793 (2012)

[24] Karas M, Hillenkamp F, Anal. Chem., 60, 2299 (1988)

[25] Krishnamurthy T, Rajamani U, Ross PL, Rapid Commun. Mass Spectrom., 10, 883 (1996)

[26] El-Sayed MA, Acc. Chem. Res., 34, 257 (2001)

[27] Fournelle M, Bost W, Tarner IH, Lehmberg T, Weiß E, Lemor R, Dinser R, Nanomed.: Nanotechnol. Biol. Med., 8, 346 (2012)

[28] Bhaisare ML, Abdelhamid HN, Wua BS, Wu HF, J. Mater. Chem. B, 2, 4671 (2014)

[29] Wu BS, Abdelhamid HN, Wu HF, RSC Adv., 4, 3722 (2014)

[30] Liu JC, Chen WJ, Li CW, Mong KK, Tsai PJ, Tsai TL, Lee YC, Chen YC, Analyst, 134, 2087 (2009)

[31] Judy G, Hasan N, Manikandan M, Wu HF, Sci. Rep., 3, 1260 (2013)

[32] Judy G, Hasan N, Wu HF, Biosens. Bioelectron., 39, 57 (2013)

[33] Dilip KR, Brien NG, Britta L, Gunvor A, William JG, J. Mass Spectrom., 39, 289 (2004)

[34] Michael TS, Hiroki T, Andre JO, Infect. Immun., 79, 459 (2011)

[35] Bohannon RW, Phys. Ther., 63, 1622 (1983)

[36] Hamblin MR, Mechanism of low level light therapy. hwww.photobiology.info.hamblin.html www.mgh.harvard.edu/wellman/people/mhamblin.asp>.

[37] Duan R, Li TCY, Guo H, Yao LB, Lasers Surg. Med., 29, 174 (2001)

[38] Poon VK, Huang L, Burd A, J. Photochem. Photobiol. B-Biol., 81, 1 (2005)

[39] Hashmi JT, Huang YY, Shama SK, Taboada K, Carrol JD, Hamblin MR, Lasers Surg. Med., 42, 450 (2010)

[40] Lin ZQ, Kondo T, Ishida Y, Takayasu T, Mukaida N, J. Leukocyte Biol., 73, 713 (2003)

[41] Kipshidze N, Nikolaychik V, Keelan MH, Shankar LR, Khanna A, Kornowski R, Leon M, Moses J, Lasers Surg. Med., 28, 355 (2001)

[42] Khanna A, Shankar LR, Keelan MH, Kornowski R, Leon M, Moses KJN, Cardiovasc. Radiat. Med., 1, 265 (1999)

[43] Narayana LL, Gopal J, Wu HF, Analyst, 137, 3372 (2012)

[44] Khan MS, Gedda G, Wu HF, Gopal J, Anal. Methods, 6, 5304 (2014)

[45] Lyons RF, Abergel RP, White RA, Dwyer RM, Costel JC, J. Ann. Plast. Surg., 1847 (1987)

[46] Kesava RG, Lasers Surg. Med., 33, 344 (2003)