화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Journal of Chemical Engineering, Vol.33, No.5, 1659-1668, May, 2016
DOI10.1007/s11814-015-0298-1  Export Citation
Antibacterial and in vivo reactivity of bioactive glass and poly(vinyl alcohol) composites prepared by melting and sol-gel techniques
Salha Boulila1, 2, Hassane Oudadesse1, 3, Hafed Elfeki2, †, Rim Kallel4, Bertrand Lefeuvre1, 3, Mostafa Mabrouk5, Slim Tounsi6, Dhekra Mhalla6, Amany Mostafa5, Khansa Chaabouni7, Fatma Makni-Ayedi7, Allal Barroug8, Tahia Boudawara4, and Abdelfattah Elfeki9
  • 1University of Rennes 1, UMR CNRS 6226, Campus de Beaulieu, 35042 Rennes, France
  • 2Laboratory of Sciences Material and Environment, Faculty of Sciences of Sfax, B.P. 1171, Sfax 3000, Tunisia
  • 3, France
  • 4Anatomopathology Laboratory, CHU Habib Bourguiba 3029, University of Sfax, Sfax, Tunisia
  • 5Refractories, Ceramics and Building Materials Department, National Research Centre, 33El Bohouth St. (Former EL Tahrir st.)- Dokki- Giza- P. O. 12622, Egypt
  • 6Biopesticides Team (LPAP), Centre of Biotechnology of Sfax, Sfax University, P. O. Box 1177, Sfax 3018, Tunisia
  • 7Laboratory of Biochemical, University Hospital Habib Bourguiba, Avenue El Fardous, 3029 Sfax, Tunisia
  • 8University of Cadi Ayyad, Marrakech and CNRST Rabat, Maroc, France
  • 9Laboratory of Animal Ecophysiology, Faculty of Sciences of Sfax, University of Sfax, P. O. Box 95, Sfax 3052, Tunisia
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Bioactive glass particle is used in the repair of bone defects. This material undergoes a series of surface in vivo reactions, which leads to osteointegration. We evaluated the effect of the bioactive glass synthesis, sol-gel (BG(S)) versus melting (BG(M)), associated with polyvinyl-alcohol (PVA) on in vivo bioactivity with biochemical parameters, liver-kidney histological structure and antibacterial in vitro activity. These composites were testified in many bacteria and implanted in ovariectomized rat. The serum and organs (liver and kidney) of all groups, control and treated rats, were collected to investigate the side effects of our composites, BG(S)-PVA and BG(M)-PVA, in comparison with control and ovariectomized rats. Also, the implants, before and after implantation, were prepared for analysis using physicochemical techniques such as Fourier transform infrared spectroscopy and X-ray diffraction. Our results have shown the stability of natremia, kaliemia, calcemia and phosphoremia. The histological structures of liver and kidney in implanted rats are intact compared to control and ovariectomized rats. BG(S)-PVA is characterized by a higher antibacterial effect on negative and positive gram bacteria than BG(M)-PVA. The physicochemical results have confirmed a progressive degradation of BG(S)-PVA and BG(M)-PVA, while replacing the implant by an apatite layer. But this bioactivity of BG(S)-PVA is faster than BG(M)-PVA. We can therefore confirm, on the one hand, the biocompatibility of our two implants and, on the other hand, the beneficial effect of sol-gel synthesis technique versus melting, both on the antibacterial effect and on the rapid formation of layer hydroxyapatite, and consequently on osteogenesis.
Keywords:Antibacterial;Bioactivity;Biocompatibility;Bioactive Glass;Melting;Sol-gel
  1. Mabrouk M, Mostafa A, Oudadesse H, Wers E, Lucas-Girot A, El-Gohary MI, Bioceram. Dev. Appl., 4, 100007 (2014)
  2. Mezahi FZ, Lucas-Girot A, Oudadesse H, Harabi A, J. Non-Cryst. Solids, 361, 111 (2013)
  3. Mabrouk M, Mostafa AA, Oudadesse H, Mahmoud AA, El-Gohary MI, Ceram. Int., 40, 4833 (2014)
  4. Wiria FE, Chua CK, Leong KF, Quah ZY, Chandrasekaran M, Lee MW, J. Mater. Sci. -Mater. Med., 19, 989 (2008)
  5. Pan Y, Xiong D, Wear, 266, 699 (2009)
  6. Wang M, Li Y, Wu J, Xu F, Zuo Y, Jansen JA, J. Biomed. Mater. Res. A, 85, 418 (2008)
  7. Mortazavi V, Nahrkhalaji MM, Fathi MH, Mousavi SB, Esfahani BN, J. Biomed. Mater. Res. A, 94, 160 (2010)
  8. Mozafari M, Moztarzadeh F, Int. Ceram. Rev., 62, 423 (2014)
  9. Gholipourmalekabadi M, Sameni M, Hashemi A, Zamani F, Rostami A, Mozafari M, Silver- and fluoride-containing mesoporous bioactive glasses versus commonly used antibiotics: Activity against multidrug-resistant bacterial strains isolated from patients with burns, Burns (2015).
  10. Mozafari M, Bioceram. Dev. Appl., 4, e106 (2014)
  11. Baghbani F, Moztarzadeh F, Hajibaki L, Mozafari M, Bull. Mat. Sci., 36, 1339 (2014)
  12. Rostami A, Mozafari M, Gholipourmalekabadi M, Caicedo HH, Lasjerdi Z, Sameni M, Samadikuchaksaraei A, Acta Trop., 148, 105 (2015)
  13. Dietrich E, Oudadesse H, Lucas-Girot A, Mami M, J. Biomed. Mater. Res. A, 88, 1087 (2009)
  14. Mabrouk M, Mostapaha AA, Oudadesse H, Mahmoud AA, Gaafar AM, El-Gohary MI, Bioceram. Dev. Appl., S, 1 (2013)
  15. Gao C, Gao Q, Li Y, Rahaman MN, Teramoto A, Abe K, J. Biomed. Mater. Res. A, 100, 1324 (2012)
  16. Yazdanpanah A, Kamalian R, Moztarzadeh F, Mozafari M, Ravarian R, Tayebi L, Ceram. Int., 38, 5007 (2012)
  17. Wong SC, Baji A, Gent AN, Compos. Pt. A-Appl. Sci. Manuf., 39, 579 (2008)
  18. Jones JR, J. European Ceram. Soc., 29, 1275 (2009)
  19. Nalla RK, Kinney JH, Ritchie RO, Biomaterials, 24, 3955 (2003)
  20. Sepulveda P, Jones JR, Hench LL, J. Biomed. Mater. Res., 58, 734 (2001)
  21. Peltola T, Jokinen M, Rahiala H, Levanen E, Rosenholm JB, Kangasniemi I, Yli-Urpo A, J. Biomed. Mater. Res., 44, 12 (1999)
  22. Pereira MM, Hench LL, J. Sol-Gel Sci. Technol., 7, 59 (1996)
  23. Li R, Clark AE, Hench LL, J. Appl. Biomater., 2, 231 (1991)
  24. Boyar H, Turan B, Severcan F, J. Spectrosc., 17, 627 (2003)
  25. Jebahi S, Oudadesse H, Xv B, Keskes H, Rebai T, El Feki A, El Feki H, Afr. J. Pharm. Pharmacol., 6, 1276 (2012)
  26. Jones JR, Acta Biomater., 9, 4457 (2013)
  27. Shin SH, Kim HI, J. Ind. Eng. Chem., 7(3), 147 (2001)
  28. Farlay D, Panczer G, Rey C, Delmas PD, Boivin G, J. Bone. Miner. Metab., 28, 433 (2010)
  29. Krishnan V, Lakshmi T, J. Adv. Pharm. Technol. Res., 4, 78 (2013)
  30. Yacoub YI, Amine B, Laatiris A, Wafki F, Znat F, Hajjaj-Hassouni N, Rheumatol. Int., 32, 3143 (2012)
  31. Bretcanu O, Misra S, Roy I, Renghini C, Fiori F, Boccaccini AR, Salih V, J. Tissue. Eng. Regen. Med., 3, 139 (2009)
  32. Kim HG, Fibers Polym., 12, 602 (2011)
  33. Liao H, Qi R, Shen M, Cao X, Guo R, Zhang Y, Shi X, Colloids Surf. B: Biointerfaces, 84, 528 (2011)
  34. Echezarreta-Lopez MM, Landin M, Int. J. Pharm., 453, 641 (2013)
  35. Allan I, Newman H, Wilson M, Biomaterials, 22, 1683 (2001)
  36. Zhang D, Lepparanta O, Munukka E, Ylanen H, Viljanen MK, Eerola E, Hupa M, Hupa L, J. Biomed. Mater. Res. A, 93, 475 (2010)
  37. Nikaido H, J. Bioenerg. Biomembr., 25, 581 (1993)
  38. Nikaido H, Microbiol. Mol. Biol. Rev. MMBR, 67, 593 (2003)