Selection of Medium Components by Plackett-Burman Design for Cell Growth of a Newly Isolated Methylobacterium sp. WJ4

Wangjun Lee; Jinwon Lee

Korean Chemical Engineering Research, Vol.54, No.6, 812-816, December, 2016

DOI10.9713/kcer.2016.54.6.812 Export Citation

Selection of Medium Components by Plackett-Burman Design for Cell Growth of a Newly Isolated Methylobacterium sp. WJ4

Wangjun Lee, and Jinwon Lee^†

Department of Chemical and Biomolecular Engineering, Sogang University, 35, Baekbeom-ro, Mapo-gu, Seoul, 04107, Korea

E-mail:

We isolated a novel methylotrophic bacterium from soil collected in Dongducheon Environment Affairs Agency. The isolate was identified as Methylobacterium sp. WJ4 based on phylogenetic analysis. Plackett-Burman design was employed for screening eight parameters of nitrate mineral salts (NMS) medium for cell growth of a newly isolated Methylobacterium sp. WJ4 with experimental validation. Trace element solution and vitamin stock were found to affect cell growth, which can be further optimized for increased cell growth. This is the first report of screening parameters of NMS medium which affect cell growth of strain belonging to the genus Methylobacterium using Plackett-Burman design.

Keywords:Cell growth;Isolation;Methylobacterium;NMS medium;Plackett-Burman design

[References]

Hou CT, Methylotrophs: Microbiology, biochemistry, and genetics, CRC pp. 1-53(1984).
Faust U, Prave H, Biotechnology. VCH Weinheim, 3, 84 (1991)
Kim P, Kim JH, Oh DK, World J. Microbiol. Biotechnol., 19, 357 (2003)
Schrader J, Schilling M, Holtmann D, Sell D, Filho MV, Marx A, Vorholt JA, Trends Biotechnol., 27, 107 (2009)
Green PN, Methylobacterium, The prokaryotes, Springer, pp.257-265(2006).
Corpe W, Rheem S, FEMS Microbiol. Ecol., 5, 243 (1989)
Hirano SS, Upper CD, “Bacterial Community Dynamics, Microbial Ecology of Leaves,” Springer, pp. 271-294(1991).
Sirirote P, Tsuneo Y, Shoichi S, J. Ferment. Technol., 66, 291 (1988)
Belanger L, Figueira M, Bourque D, Morel L, Beland M, Laramee L, Groleau D, Miguez C, FEMS Microbiol. Lett., 231, 197 (2004)
Choi YJ, Gringorten JL, Belanger L, Morel L, Bourque D, Masson L, Groleau D, Miguez CB, Appl. Environ. Microbiol., 74, 5178 (2008)
Carroll SM, Chubiz LM, Agashe D, Marx CJ, Microorganisms, 3, 152 (2015)
Stanbury PF, Whitaker A, Hall SJ, Principles of fermentation technology, Elsevier(2013).
Quan Y, Wang L, Liu Y, Cong J, Xie S, Wu X, Korean Chem. Eng. Res., 53(3), 321 (2015)
Li C, Bai J, Cai Z, Ouyang F, J. Biotechnol., 93, 27 (2002)
Box GE, Hunter JS, Hunter WG, Statistics for Experimenters: Design, Innovation, and Discovery, Wiley-Interscience New York(2005).
Delaney NF, Kaczmarek ME, Ward LM, Swanson PK, Lee MC, Marx CJ, PLoS One, 8, e62957 (2013)
Whittenbury R, Phillips K, Wilkinson J, Microbiology, 61, 205 (1970)
Dedysh SN, Dunfield PF, Cultivation of Methanotrophs, Hydrocarbon and Lipid Microbiology Protocols, Springer protocols handbooks. Berlin: Springer-Verlag (2014).
Sanger F, Nicklen S, Coulson AR, Proc. Natl. Acad. Sci. U.S.A., 74, 5463 (1977)
Kumar S, Stecher G, Tamura K, Mol. Biol. Evol., 1870 (2016)
Crolla A, Kennedy K, J. Biotechnol., 89, 27 (2001)
Abdel-Fattah YR, Saeed HM, Gohar YM, El-Baz MA, Process Biochem., 40(5), 1707 (2005)

[Referenced By]

[1] Hou CT, Methylotrophs: Microbiology, biochemistry, and genetics, CRC pp. 1-53(1984).

[2] Faust U, Prave H, Biotechnology. VCH Weinheim, 3, 84 (1991)

[3] Kim P, Kim JH, Oh DK, World J. Microbiol. Biotechnol., 19, 357 (2003)

[4] Schrader J, Schilling M, Holtmann D, Sell D, Filho MV, Marx A, Vorholt JA, Trends Biotechnol., 27, 107 (2009)

[5] Green PN, Methylobacterium, The prokaryotes, Springer, pp.257-265(2006).

[6] Corpe W, Rheem S, FEMS Microbiol. Ecol., 5, 243 (1989)

[7] Hirano SS, Upper CD, “Bacterial Community Dynamics, Microbial Ecology of Leaves,” Springer, pp. 271-294(1991).

[8] Sirirote P, Tsuneo Y, Shoichi S, J. Ferment. Technol., 66, 291 (1988)

[9] Belanger L, Figueira M, Bourque D, Morel L, Beland M, Laramee L, Groleau D, Miguez C, FEMS Microbiol. Lett., 231, 197 (2004)

[10] Choi YJ, Gringorten JL, Belanger L, Morel L, Bourque D, Masson L, Groleau D, Miguez CB, Appl. Environ. Microbiol., 74, 5178 (2008)

[11] Carroll SM, Chubiz LM, Agashe D, Marx CJ, Microorganisms, 3, 152 (2015)

[12] Stanbury PF, Whitaker A, Hall SJ, Principles of fermentation technology, Elsevier(2013).

[13] Quan Y, Wang L, Liu Y, Cong J, Xie S, Wu X, Korean Chem. Eng. Res., 53(3), 321 (2015)

[14] Li C, Bai J, Cai Z, Ouyang F, J. Biotechnol., 93, 27 (2002)

[15] Box GE, Hunter JS, Hunter WG, Statistics for Experimenters: Design, Innovation, and Discovery, Wiley-Interscience New York(2005).

[16] Delaney NF, Kaczmarek ME, Ward LM, Swanson PK, Lee MC, Marx CJ, PLoS One, 8, e62957 (2013)

[17] Whittenbury R, Phillips K, Wilkinson J, Microbiology, 61, 205 (1970)

[18] Dedysh SN, Dunfield PF, Cultivation of Methanotrophs, Hydrocarbon and Lipid Microbiology Protocols, Springer protocols handbooks. Berlin: Springer-Verlag (2014).

[19] Sanger F, Nicklen S, Coulson AR, Proc. Natl. Acad. Sci. U.S.A., 74, 5463 (1977)

[20] Kumar S, Stecher G, Tamura K, Mol. Biol. Evol., 1870 (2016)

[21] Crolla A, Kennedy K, J. Biotechnol., 89, 27 (2001)

[22] Abdel-Fattah YR, Saeed HM, Gohar YM, El-Baz MA, Process Biochem., 40(5), 1707 (2005)