Development of fluorescence-conjugated islet-homing peptide using biopanning for targeted optical imaging of pancreatic islet

Min Jun Kim; Jeong Heon Yu; Mi Hwa Oh; Yoon Sung Nam; Dong Yun Lee

Journal of Industrial and Engineering Chemistry, Vol.45, 404-412, January, 2017

DOI10.1016/j.jiec.2016.10.009 Export Citation

Development of fluorescence-conjugated islet-homing peptide using biopanning for targeted optical imaging of pancreatic islet

Min Jun Kim, Jeong Heon Yu¹, Mi Hwa Oh², Yoon Sung Nam^{1, †}, and Dong Yun Lee^†

Departments of Bioengineering, College of Engineering, and BK21 PLUS Future Biopharmaceutical Human Resources Training and Research Team, and Institute of Nano Science & Technology (INST), Hanyang University, Seoul 04763, Republic of Korea
¹Departments of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea
²Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea

E-mail:,

Visualization of pancreatic islet can provide important information for diagnosis and monitoring of pancreatic islets. By using phage display technology, here we newly identified four candidate peptide sequences with islet-homing property. In vitro binding assay showed that LSALPRT peptide had stronger affinity with islet cells than other candidates. Then this islet-homing peptide (IHP) was chemically conjugated with TAMRA fluorophore for near infrared (NIR) imaging of islet cells. This TMARA-IHP was strongly bound to islets in vitro, but also to cryo-sectioned slice of pancreas tissue ex vivo. Collectively, it would be a considerable potential as therapeutics or diagnostics in future studies.

Keywords:Islet-homing peptide;Pancreatic islet;Phage display;Biopanning;Islet imaging

[References]

Staeva-Vieira T, Peakman M, von Herrath M, Clin. Exp. Immunol., 149, 203 (2007)
Mukai E, Toyoda K, Kimura H, Kawashima H, Fujimoto H, Ueda M, Temma T, Hirao K, Nagakawa K, Saji H, Inagaki N, Biochem. Biophys. Res. Commun., 389(3), 523 (2009)
Mathis D, Vence L, Benoist C, Nature, 414, 792 (2001)
Malaisse WJ, Zhang Y, Louchami K, Sharma S, Dresselaers T, Himmelreich U, Novotny GW, Mandrup-Poulsen T, Waschke D, Leshch Y, Thimm J, Thiem J, Sener A, Arch. Biochem. Biophys., 517, 138 (2012)
Schottelius M, Wester HJ, Reubi JC, Senekowitsch-Schmidtke R, Schwaiger M, Bioconjugate Chem., 13, 1021 (2002)
Zhang B, Yang B, Zhai CX, Jiang B, Wu YL, Biomaterials, 34, 5843 (2013)
Kulkarni RN, Int. J. Biochem. Cell Biol., 36, 365 (2004)
Smith GP, Science, 228, 1315 (1985)
Arap W, Kolonin MG, Trepel M, Lahdenranta J, Cardo-Vila M, Giordano RJ, Mintz PJ, Ardelt PU, Yao VJ, Vidal CI, Chen L, Flamm A, Valtanen H, Weavind LM, Hicks ME, Pollock RE, Botz GH, Bucana CD, Koivunen E, Cahill D, Troncoso P, Baggerly KA, Pentz RD, Do K, Nat. Med., 8, 121 (2002)
Kolonin MG, Saha PK, Chan L, Pasqualini R, Arap W, Nat. Med., 10, 625 (2004)
Joyce JA, Laakkonen P, Bernasconi M, Bergers G, Ruoslahti E, Hanahan D, Cancer Cell, 4, 393 (2003)
Samli KN, McGuire MJ, Newgard CB, Johnston SA, Brown KC, Diabetes, 54, 2103 (2005)
Yao VJ, Ozawa MG, Trepel M, Arap W, McDonald DM, Pasqualini R, Am. J. Pathol., 166, 625 (2005)
Jung HS, Lim KS, Kim MJ, Hwang YH, Yoo C, Lee YK, Kim YH, Lee DY, J. Control. Release, 172, 1092 (2013)
Sutton R, Peters M, Mcshane P, Gray DWR, Morris PJ, Transplantation, 42, 689 (1986)
Swaminathan S, Cui Y, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 33, 3082 (2013)
Ueberberg S, Meier JJ, Schechinger W, Schmitz I, Tannapfel A, Schirrmacher R, Hermann S, Koller M, Klein HH, Schneider S, Diabetes, 58, A92 (2009)
Behr TM, Gotthardt M, Barth A, Behe M, Q. J. Nucl. Med., 45, 189 (2001)
Reubi JC, Endocr. Rev., 24, 389 (2003)
Wu Z, Liu S, Hassink M, Nair I, Park R, Li L, Todorov I, Fox JM, Li Z, Shively JE, Conti PS, Kandeel F, J. Nucl. Med., 54, 244 (2013)
Wu Z, Todorov I, Li L, Bading JR, Li Z, Nair I, Ishiyama K, Colcher D, Conti PE, Fraser SE, Shively JE, Kandeel F, Bioconjugate Chem., 22, 1587 (2011)

[1] Staeva-Vieira T, Peakman M, von Herrath M, Clin. Exp. Immunol., 149, 203 (2007)

[2] Mukai E, Toyoda K, Kimura H, Kawashima H, Fujimoto H, Ueda M, Temma T, Hirao K, Nagakawa K, Saji H, Inagaki N, Biochem. Biophys. Res. Commun., 389(3), 523 (2009)

[3] Mathis D, Vence L, Benoist C, Nature, 414, 792 (2001)

[4] Malaisse WJ, Zhang Y, Louchami K, Sharma S, Dresselaers T, Himmelreich U, Novotny GW, Mandrup-Poulsen T, Waschke D, Leshch Y, Thimm J, Thiem J, Sener A, Arch. Biochem. Biophys., 517, 138 (2012)

[5] Schottelius M, Wester HJ, Reubi JC, Senekowitsch-Schmidtke R, Schwaiger M, Bioconjugate Chem., 13, 1021 (2002)

[6] Zhang B, Yang B, Zhai CX, Jiang B, Wu YL, Biomaterials, 34, 5843 (2013)

[7] Kulkarni RN, Int. J. Biochem. Cell Biol., 36, 365 (2004)

[8] Smith GP, Science, 228, 1315 (1985)

[9] Arap W, Kolonin MG, Trepel M, Lahdenranta J, Cardo-Vila M, Giordano RJ, Mintz PJ, Ardelt PU, Yao VJ, Vidal CI, Chen L, Flamm A, Valtanen H, Weavind LM, Hicks ME, Pollock RE, Botz GH, Bucana CD, Koivunen E, Cahill D, Troncoso P, Baggerly KA, Pentz RD, Do K, Nat. Med., 8, 121 (2002)

[10] Kolonin MG, Saha PK, Chan L, Pasqualini R, Arap W, Nat. Med., 10, 625 (2004)

[11] Joyce JA, Laakkonen P, Bernasconi M, Bergers G, Ruoslahti E, Hanahan D, Cancer Cell, 4, 393 (2003)

[12] Samli KN, McGuire MJ, Newgard CB, Johnston SA, Brown KC, Diabetes, 54, 2103 (2005)

[13] Yao VJ, Ozawa MG, Trepel M, Arap W, McDonald DM, Pasqualini R, Am. J. Pathol., 166, 625 (2005)

[14] Jung HS, Lim KS, Kim MJ, Hwang YH, Yoo C, Lee YK, Kim YH, Lee DY, J. Control. Release, 172, 1092 (2013)

[15] Sutton R, Peters M, Mcshane P, Gray DWR, Morris PJ, Transplantation, 42, 689 (1986)

[16] Swaminathan S, Cui Y, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 33, 3082 (2013)

[17] Ueberberg S, Meier JJ, Schechinger W, Schmitz I, Tannapfel A, Schirrmacher R, Hermann S, Koller M, Klein HH, Schneider S, Diabetes, 58, A92 (2009)

[18] Behr TM, Gotthardt M, Barth A, Behe M, Q. J. Nucl. Med., 45, 189 (2001)

[19] Reubi JC, Endocr. Rev., 24, 389 (2003)

[20] Wu Z, Liu S, Hassink M, Nair I, Park R, Li L, Todorov I, Fox JM, Li Z, Shively JE, Conti PS, Kandeel F, J. Nucl. Med., 54, 244 (2013)

[21] Wu Z, Todorov I, Li L, Bading JR, Li Z, Nair I, Ishiyama K, Colcher D, Conti PE, Fraser SE, Shively JE, Kandeel F, Bioconjugate Chem., 22, 1587 (2011)