Gelatin-based extracellular matrix cryogels for cartilage tissue engineering

Min-Eui Han; Byung Jae Kang; Su-Hwan Kim; Hwan D. Kim; Nathaniel S. Hwang

Journal of Industrial and Engineering Chemistry, Vol.45, 421-429, January, 2017

DOI10.1016/j.jiec.2016.10.011 Export Citation

Gelatin-based extracellular matrix cryogels for cartilage tissue engineering

Min-Eui Han¹, Byung Jae Kang², Su-Hwan Kim^{1, 3}, Hwan D. Kim³, and Nathaniel S. Hwang^{1, 3, †}

¹Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, Republic of Korea
²School of Veterinary Medicine, Kangwon National University, Chuncheon, Kangwon Province, Republic of Korea
³School of Chemical and Biological Engineering, Institute of Chemical Processes, N-Bio Institute, Seoul National University, Seoul, Republic of Korea

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In this study, gelatin-based cryogels were fabricated by mixing methacrylated gelatin (GelMA) with methacrylated hyaluronic acid (MeHA) or methacrylated chondroitin sulfate (MeCS) for cartilage tissue engineering. In vitro revealed that MeCS incorporated gelatin-based cryogel (G-MeCS) showed significant cartilaginous tissue stimulation. Furthermore, the cell-laden gelatin-based ECM cryogels were implanted into mouse subcutaneous tissue for 6 weeks and displayed uniform distribution of cells with normal phenotype maintenance. Finally, when these cryogels were implanted into osteochondral defect of New Zealand white rabbit, full integration with host tissue and increased cellularity were observed with GMeCS cryogel.

Keywords:Cryogel;Chondroitin sulfate;Cartilage;Tissue engineering;Osteochondral

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Lou X, Chirila TV, J. Biomater. Appl., 14(2), 184 (1999)
Ruoslahti E, Pierschbacher MD, Cell, 44(4), 517 (1986)
Chang CH, et al., Biomaterials, 24(26), 4853 (2003)
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Chandran PL, Horkay F, Acta Biomater., 8(1), 3 (2012)
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[Referenced By]

[1] Hwang NS, Elisseeff J, J. Knee Surg., 22(1), 60 (2009)

[2] Cancedda R, et al., Matrix Biol., 22(1), 81 (2003)

[3] Sharma B, Elisseeff JH, Ann. Biomed. Eng., 32(1), 148 (2004)

[4] Hwang NS, et al., Febs Lett., 581(22), 4172 (2007)

[5] Qu D, et al., Ann. Biomed. Eng., 43(3), 697 (2015)

[6] Han ME, et al., Int. J. Biol. Macromol. (2016)

[7] Hwang Y, Sangaj N, Varghese S, Tissue Eng. A, 16(10), 3033 (2010)

[8] Kathuria N, et al., Acta Biomater., 5(1), 406 (2009)

[9] Bhat S, Kumar A, Biomatter, 3(3) (2013)

[10] Bhat S, Lidgren L, Kumar A, Macromol. Biosci., 13(7), 827 (2013)

[11] Kuo CY, et al., Carbohydr. Polym., 117, 722 (2015)

[12] Salgado CL, et al., J. Biomed. Mater. Res., 104(1), 57 (2016)

[13] Gupta A, et al., Tissue Eng. A, 20(23-24), 3101 (2014)

[14] Van Vlierberghe S, et al., J. Control. Release, 116(2), e95 (2006)

[15] Oelschlaeger C, Bossler F, Willenbacher N, Biomacromolecules, 17(2), 580 (2016)

[16] Kim HD, Tissue Eng. A, 21(3-4), 757 (2015)

[17] Lou X, Chirila TV, J. Biomater. Appl., 14(2), 184 (1999)

[18] Ruoslahti E, Pierschbacher MD, Cell, 44(4), 517 (1986)

[19] Chang CH, et al., Biomaterials, 24(26), 4853 (2003)

[20] Ghasemi-Mobarakeh L, et al., Biomaterials, 29(34), 4532 (2008)

[21] Benton JA, et al., Tissue Eng. A, 15(11), 3221 (2009)

[22] Kenchington AW, Biochem. J., 68, 458 (1958)

[23] Einerson NJ, Stevens KR, Kao WYJ, Biomaterials, 24(3), 509 (2003)

[24] Phillips JM, Kao WJ, Tissue Eng., 11(5-6), 964 (2005)

[25] Son TI, et al., Acta Biomater., 6(10), 4005 (2010)

[26] Mazaki T, et al., Sci. Rep., 4, 4457 (2014)

[27] Discher DE, Janmey P, Wang YL, Science, 310(5751), 1139 (2005)

[28] Enobakhare BO, Bader DL, Lee DA, Anal. Biochem., 243(1), 189 (1996)

[29] Cohen NP, Foster RJ, Mow VC, J. Orthop. Sports Phys. Ther., 28(4), 203 (1998)

[30] Gorgieva S, Kokol V, J. Biomed. Mater. Res. A, 103(3), 1119 (2015)

[31] Muzzarelli RA, et al., Carbohydr. Polym., 89(3), 723 (2012)

[32] Van Vlierberghe S, et al., J. Biomater. Sci.-Polym. Ed., 20(10), 1417 (2009)

[33] Kang HW, Tabata Y, Ikada Y, Biomaterials, 20(14), 1339 (1999)

[34] Wu X, et al., Acta Biomater., 6(3), 1167 (2010)

[35] Chandran PL, Horkay F, Acta Biomater., 8(1), 3 (2012)

[36] Comper WD, Laurent TC, Physiol. Rev., 58(1), 255 (1978)

[37] Mendler M, et al., J. Cell Biol., 108(1), 191 (1989)

[38] Gelse K, Poschl E, Aigner T, Adv. Drug Deliv. Rev., 55(12), 1531 (2003)