Comparison of metabolite profiling of Ralstonia eutropha H16 phaBCA mutants grown on different carbon sources

Dae-Kyun Im; Seok Hun Yun; Joon-Young Jung; Jinwon Lee; Min-Kyu Oh

Korean Journal of Chemical Engineering, Vol.34, No.3, 797-805, March, 2017

DOI10.1007/s11814-016-0333-x Export Citation

Comparison of metabolite profiling of Ralstonia eutropha H16 phaBCA mutants grown on different carbon sources

Dae-Kyun Im¹, Seok Hun Yun^{2, 3}, Joon-Young Jung^{2, 4}, Jinwon Lee^{3, †}, and Min-Kyu Oh^{1, †}

¹Department of Chemical and Biological Engineering, Korea University, Seoul 02841, Korea
²CJ Research Institute of Biotechnology, Suwon 16495, Korea
³Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 04107, Korea
⁴, Korea

E-mail:,

Metabolite profiling was conducted to monitor the metabolic differences of the Ralstonia eutropha H16, polyhydroxybutyrate (PHB) producing strain, when grown on different sole carbon sources and to identify the effect of the deletion of the PHB production pathway. The combination of rapid filtration and boiling ethanol extraction successfully extracted intracellular metabolites. Accumulation of acetyl-CoA was detected when the strain was grown on fructose. Whereas, lower adenylate energy charge (AEC) ratio and cell mass was identified on the cells grown on acetate. No significant difference was detected between the wild type and mutant strain except metabolites in the PHB synthetic pathway. Increased PHB production is expected on fructose due to the accumulation of AcCoA, while it is more difficult to improve the production on acetate because of lower metabolic precursor level and AEC ratio.

Keywords:Ralstonia eutropha H16;PHA;Metabolite Profiling;GC-EI-MS;LC/TOF/MS;Adenylate Energy Charge(AEC) Ratio

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