A thermo-responsive separation membrane was synthesized by covering inorganic porous membrane, a glass filter (GF), with poly(N-isopropylacrylamide) (PNIPAM). The temperature dependence of the permeation coefficients of methyl orange (MO) and congo red (CR) through the membrane were evaluated, and the effect of adding the cross-linking agent, N,N'-methylenebisacrylamide (BIS) on the separation was examined. The amount of PNIPAM immobilized on the GF was measured to be 0.3-1.6 wt% by thermogravimetric analysis and weight analysis. The permeation coefficients of MO and CR for GF were respectively 2.9x10(-7) m/s and 2.0x10(-7) m/s at 25 degrees C, and 3.0x10(-7) m/s and 2.3x10(-7) m/s at 40 degrees C. The ratios of permeation coefficients at 25 degrees C and 40 degrees C (K=k(40)/k(25)) for MO and CR were 1.0 and 1.2, respectively. When the GF was covered with PNIPAM (PGF), the ratio (K) for MO was 2.2. This behavior was considered to be related to the volume change of PNIPAM immobilized on the GF with the change in temperature. Moreover, the addition of BIS to PNIPAM resulted in the further increase in the ratio (K). In the case of CR, the ratio (K) significantly increased to 5.8-7.4 by adding more than 5 wt% of BIS to PNIPAM.