Accelerated osteointegration of the titanium-implant coated with biocomponents, collagen/hydroxyapatite/bone morphogenetic protein-2, for bone-anchored hearing aid

Chul Ho Jang; Hyeongjin Lee; Minseong Kim; Geun Hyung Kim

Journal of Industrial and Engineering Chemistry, Vol.63, 230-236, July, 2018

DOI10.1016/j.jiec.2018.02.019 Export Citation

Accelerated osteointegration of the titanium-implant coated with biocomponents, collagen/hydroxyapatite/bone morphogenetic protein-2, for bone-anchored hearing aid

Chul Ho Jang^†, Hyeongjin Lee¹, Minseong Kim², and Geun Hyung Kim^{2, †}

Department of Otolaryngology, Chonnam National University Medical School, Gwangju, South Korea
¹bWake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157, USA
²Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon, South Korea

E-mail:,

A non-skin-penetrating bone-anchored hearing aid (BAHA-attract) with a single-point fixation system was recently developed for single-side deafness or sensorineural hearing loss. Here, we coated hydroxyapatitite (HA)/bone morphogenetic protein-2 (BMP-2)/collagen on the implant screw. To evaluate the osteogenesis induced by the modified titanium implant, we conducted in vitro and in vivo tests. The implant showed increased cell-proliferation of 2.3-fold greater than the control, and ALP activity/calcium mineralization results indicated enhanced osteogenic differentiation. According to oxytetracycline and alizarin expression results, the best enhanced peri-implant bone formation (87 ± 5.2%) was observed in the implant compared to the other groups.

Keywords:Bone anchored hearing aid;Implant;Bone morphogenetic protein-2;Collagen;Osteointegration

[References]

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Monksfield P, Chapple IL, Matthews JB, Grant MM, Addison O, Reid AP, Proops DW, Sammons RL, J. Laryngol. Otol. Suppl., 125, 1125 (2011)
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Jang CH, Kim MS, Cho YB, Jang YS, Kim GH, Int. J. Biol. Macromol., 62, 614 (2013)
Jang YS, Choi CH, Cho YB, Kang MK, Jang CH, Acta Otolaryngol., 134, 785 (2014)
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Kim G, Ahn S, Yoon H, Kim Y, Chun W, J. Mater. Chem., 19, 8817 (2009)
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[Referenced By]

[1] Snik AF, Mylanus EA, Proops DW, Wolfaardt JF, Hodgetts WE, Somers T, Niparko JK, Wazen JJ, Sterkers O, Cremers CW, Tjellstrom A, Ann. Otol. Rhinol. Laryngol. Suppl., 195, 2 (2005)

[2] Desmet J, Bouzegta R, Hofkens A, Backer AD, Lambrechts P, Wouters K, Claes J, Bodt MD, Heyning PVD, Eur. Arch. Otorhinolaryngol., 269, 799 (2012)

[3] Janssen RM, Hong P, Chadha NK, Otolaryngol. Head Neck Surg., 147, 412 (2012)

[4] Kurz A, Flynn M, BioMed. Res. Int., 2014, 416205 (2014)

[5] Iseri M, Orhan KS, Kara A, Durgut M, Ozturk M, Topdag M, Caliskan S, Kulak Burun Bogaz Ihtis. Derg., 24, 59 (2014)

[6] Clamp PJ, Briggs RJ, Expert Rev. Med. Devices, 12, 223 (2015)

[7] Monksfield P, Chapple IL, Matthews JB, Grant MM, Addison O, Reid AP, Proops DW, Sammons RL, J. Laryngol. Otol. Suppl., 125, 1125 (2011)

[8] Iseri M, Orhan KS, Tuncer U, Kara A, Durgut M, Guldiken Y, Surmelioglu O, Otol. Neurotol., 36, 849 (2015)

[9] Alsaadi G, Quirynen M, Michiles K, Teughels W, Komarek A, van Steenberghe D, J. Clin. Periodontol., 35, 51 (2008)

[10] Zeitoun H, De R, Thompson SD, Proops DW, J. Laryngol. Otol. Suppl., 116, 87 (2002)

[11] Mlynski R, Goldberg E, Ebmeyer J, Scheich M, Gattenlohner S, Schwager K, Hagen R, Shehata-Dieler W, Eur. Arch. Otorhinolaryngol., 266, 745 (2009)

[12] Drinias V, Granstrom G, Tjellstrom A, Clin. Implant Dent. Relat. Res., 9, 94 (2007)

[13] Polimeni G, Wikesjo UM, Susin C, Qahash M, Shanaman RH, Prasad HS, Rohrer MD, J. Clin. Periodontol., 37, 759 (2010)

[14] Sterling JA, Guelcher SA, Curr. Osteoporos. Rep., 12, 48 (2014)

[15] Jang CH, Kim MS, Cho YB, Jang YS, Kim GH, Int. J. Biol. Macromol., 62, 614 (2013)

[16] Jang YS, Choi CH, Cho YB, Kang MK, Jang CH, Acta Otolaryngol., 134, 785 (2014)

[17] Wee S, Gombotz WR, Adv. Drug Deliv. Rev., 31, 267 (1998)

[18] Jang CH, Ahn SH, Kim GH, Int. J. Biol. Macromol., 93, 1612 (2016)

[19] Tronci G, Doyle A, Russell SJ, Wood DJ, J. Mater. Chem. B, 1, 5478 (2013)

[20] Kim G, Ahn S, Yoon H, Kim Y, Chun W, J. Mater. Chem., 19, 8817 (2009)

[21] Olivares-Navarrete R, Raines AL, Hyzy SL, Park JH, Hutton DL, Cochran DL, Boyan BD, Schwartz Z, J. Bone Miner. Res., 27, 1773 (2012)

[22] Tucci M, Lancaster R, Wingerter S, Woodall J, Russell G, Benghuzzi H, Biomed. Sci. Instrum., 45, 407 (2009)

[23] Sommerlad S, Mackenzie D, Johansson C, Atwell R, Clin. Implant Dent. Relat. Res., 9, 22 (2007)

[24] Kung S, Devlin H, Fu E, Ho KY, Liang SY, Hsieh YD, J. Periodontal. Res., 46, 126 (2011)

[25] Becker D, Geissler U, Hempel U, Bierbaum S, Scharnweber D, Worch H, Wenzel KW, J. Biomed. Mater. Res., 59, 516 (2002)

[26] Pham L, Beyer K, Jensen ED, Rodriguez JS, Davydova J, Yamamoto M, Petryk A, Gopalakrishnan R, Mansky KC, J. Cell. Biochem., 112, 793 (2011)

[27] Jensen ED, Pham L, Billington CJ, Espe K, Carlson AE, Westendorf JJ, Petryk A, Gopalakrishnan R, Mansky K, J. Cell. Biochem., 109, 672 (2010)

[28] Kaneko H, Arakawa T, Mano H, Kaneda T, Ogasawara A, Nakagawa M, Toyama Y, Yabe Y, Kumegawa M, Hakeda Y, Bone, 27, 479 (2000)