This paper presents a novel one-step method for the periodical nanopatterning and reduction of graphene oxide (GO). Self-organized periodic structures of reduced graphene oxide (rGO) appear on GO surfaces upon processing with a femtosecond laser at fluences slightly higher than the fluence needed for reduction of the GO. This indicates that the periodic pattern is formed either simultaneously with or due to the reduction of the GO. The laser-induced reduction of GO was identified by sheet resistance measurements, Raman and X-ray photoelectron spectroscopy. This fast and simple method to both reduce and periodically structure GO offers a variety of possible applications in printed and flexible electronics. (C) 2018 Elsevier B.V. All rights reserved.