Extraction of ethanol from fermentation broth was investigated under different operating conditions (313 K, 100-140 atm, 0.5-7.5 ml feed CO2 min(-1)), in a system which is continuous in terms of CO2, and batch in terms of fermentation broth. The experimental system consists of mainly four parts: the CO2 storage system, the high pressure liquid pump, the extractor and the product collection unit. Samples were analysed by a gas chromatograph. The main objective of the extraction experiments was to explore the effects of CO2 feed rate, extraction time and pressure on the extraction yield. Experiments that were carried out at 313 K, under 100 atm pressure and at seven different CO2 feed rates demonstrated that the extraction yield is closely related to the CO2 feed rate. While 13% of the initial ethanol load was extracted at 0.75 ml min(-1) CO2 feed rate after 5 h, 72% extraction was accomplished at 7.5 ml min(-1) and at the same extraction time. It was also observed that extraction yield is a close function of extraction time. The effect of pressure on the extraction yield was investigated at 313 K under 100, 120 and 140 atm pressures. Although a pressure effect has not been established yet, there is some evidence that the increased pressures help to achieve higher yields.