The purpose of the present study is to demonstrate the applicability of SFE for the separation of enantiomeric acid mixtures. (+/-)-cis- and (+/-)-trans-permetric acids were resolved with R-(+)-alpha-phenylethylamine and S-(+)2-benzylaminobutan-1-ol, respectively. The effects of temperature and pressure on the quantity and quality of cis- and trans-acid resolution products are demonstrated. Partial resolutions of the acids were investigated in the range 100-200 bar and 33-47 degrees C by designed experiments.