어류 부산물을 이용한 흡수성 연조직 접착제 개발

김 진; 임태준; 김수관; 이기영; Jin Kim; Tae Joon Im; Su-Gwan Kim; Ki-Young Lee

Polymer(Korea), Vol.43, No.3, 372-378, May, 2019

DOI10.7317/pk.2019.43.3.372 Export Citation

어류 부산물을 이용한 흡수성 연조직 접착제 개발

Development of Fish Byproduct Extract Based Absorbable Soft Tissue Bioadhesive

김 진, 임태준¹, 김수관^†, 이기영^{2, †}

조선대학교 치과대학 구강악안면외과학교실 & 조선대학교 치의학연구원
¹전남대학교 신화학소재공학과
²전남대학교 화학공학부

Jin Kim, Tae Joon Im¹, Su-Gwan Kim^†, and Ki-Young Lee^{2, †}

Department of Oral and Maxillofacial Surgery, College of Dentistry, Chosun University & Institute of Dental Science, Chosun University, Gwangju 61452, Korea
¹Department of Advanced Chemicals and Engineering, Chonnam National University, Gwangju 61186, Korea
²School of Chemical Engineering, Chonnam National University, Gwangju 61186, Korea

E-mail:,

초록

어류 부산물을 활용하여 생체 내 사용 가능한 연조직 접착제를 제조하였다. 민어부레와 황다랑어 껍질 추출물에서 80% 이상 천연아미노산 성분을 GCMS 분석을 통해 확인하였고 HPLC(high pressure liquid chromatography)와 FTIR(Fourier transform infrared spectroscopy), EDX(energy-dispersive X-ray spectroscopy) 분석결과 콘드로이친 설페이트가 함유된 것을 확인하였다. 접착강도를 측정한 결과 민어부레 추출물은 12.3±0.45 kPa 황다랑어 껍질 추출물에서는 27.85±0.45 kPa로 황다랑어의 구성 아미노산 함량이 2배 높은 만큼 접착력도 2배 높은 것을 관찰하였다. 랫의 피하에 주사하여 생분해성이 가능한 소재임을 평가하였고 세포독성이 없으며 연조직 접착제로 활용 가능함을 조직학적 평가를 통해 확인하였다.

In this study, we present the preparation of the glue from fish by-products extract and evaluation of its use as a absorbable soft-tissue adhesive. The contents of natural amino acids over 80% were confirmed in the fish by-product extracts. In addition, chondroitin sulfate was identified by HPLC (high pressure liquid chromatography), FTIR (Fourier transform infrared spectroscopy) and EDX (energy-dispersive X-ray spectroscopy). The adhesive strength analysis showed that the higher the amino acid content, the higher the strength. The fish by-product extracts were confirmed to be biodegradable. Histological evaluation confirmed that it was not cytotoxic and could be used as a soft tissue adhesive.

Keywords:fish byproduct;absorbable;soft tissue;bioadhesive.

[References]

Min KD, Park WH, Yoo DI, Hudson S, Textile Sci. Eng., 42, 60 (2005)
Oh JK, Lim DH, Kim SY, Kim HJ, JAIK, 9, 34 (2008)
Kim JY, Yoon JH, Kim DH, Kim SB, Korean J. Chem. Eng., 29(4), 503 (2012)
Ferreira P, Coelho JFJ, Gil MH, Int. J. Pharm., 352, 172 (2008)
Otani Y, Tabata Y, Ikada Y, Biomaterials, 17, 1387 (1996)
Toriumi DM, O’Grady K, Desai D, Bagal A, Plast. Reconstr. Surg., 102, 2209 (1998)
Kaewdang O, Benjakul S, Prodpran T, Kaewmanee T, Kishimura H, Ital. J. Food Sci., 27, 366 (2015)
Karim A, Bhat R, Food Hydrocolloids, 23, 563 (2009)
Woo JW, Yu SJ, Cho SM, Lee YB, Kim SB, Food Hydrocolloids, 22, 897 (2008)
Simson JA, Strehin IA, Allen BW, Elisseeff JH, Tissue Eng. Part A, 19, 1843 (2013)
Kim J, Jang JW, Lee CM, Lee KY, Polym. Korea, 41(2), 324 (2017)
Ghobril C, Grinstaff MW, Chem. Soc. Rev., 44, 1820 (2015)
Eastoe JE, Biochem. J., 61, 589 (1995)
Strehin I, Nahas Z, Arora K, Nguyen T, Elisseeff J, Biomaterials, 31, 2788 (2010)
Strehin I, Ambrose WM, Schein O, Salahuddin A, Elisseeff J, J. Cataract Refract. Surg., 35, 567 (2009)
Puertas-Bartolome M, Vazquez-Lasa B, Roman JS, Polymers, 10, 768 (2018)
Montanaro L, Arciola CR, Cenni E, Ciapetti G, Savioli F, Filippini F, Barsanti LA, Biomater. Res., 22, 59 (2001)
Lee JH, Jung TG, Hwang DY, Kang HG, Park JC, Han DW, Hyon SH, Biomater. Res., 15, 129 (2011)

[1] Min KD, Park WH, Yoo DI, Hudson S, Textile Sci. Eng., 42, 60 (2005)

[2] Oh JK, Lim DH, Kim SY, Kim HJ, JAIK, 9, 34 (2008)

[3] Kim JY, Yoon JH, Kim DH, Kim SB, Korean J. Chem. Eng., 29(4), 503 (2012)

[4] Ferreira P, Coelho JFJ, Gil MH, Int. J. Pharm., 352, 172 (2008)

[5] Otani Y, Tabata Y, Ikada Y, Biomaterials, 17, 1387 (1996)

[6] Toriumi DM, O’Grady K, Desai D, Bagal A, Plast. Reconstr. Surg., 102, 2209 (1998)

[7] Kaewdang O, Benjakul S, Prodpran T, Kaewmanee T, Kishimura H, Ital. J. Food Sci., 27, 366 (2015)

[8] Karim A, Bhat R, Food Hydrocolloids, 23, 563 (2009)

[9] Woo JW, Yu SJ, Cho SM, Lee YB, Kim SB, Food Hydrocolloids, 22, 897 (2008)

[10] Simson JA, Strehin IA, Allen BW, Elisseeff JH, Tissue Eng. Part A, 19, 1843 (2013)

[11] Kim J, Jang JW, Lee CM, Lee KY, Polym. Korea, 41(2), 324 (2017)

[12] Ghobril C, Grinstaff MW, Chem. Soc. Rev., 44, 1820 (2015)

[13] Eastoe JE, Biochem. J., 61, 589 (1995)

[14] Strehin I, Nahas Z, Arora K, Nguyen T, Elisseeff J, Biomaterials, 31, 2788 (2010)

[15] Strehin I, Ambrose WM, Schein O, Salahuddin A, Elisseeff J, J. Cataract Refract. Surg., 35, 567 (2009)

[16] Puertas-Bartolome M, Vazquez-Lasa B, Roman JS, Polymers, 10, 768 (2018)

[17] Montanaro L, Arciola CR, Cenni E, Ciapetti G, Savioli F, Filippini F, Barsanti LA, Biomater. Res., 22, 59 (2001)

[18] Lee JH, Jung TG, Hwang DY, Kang HG, Park JC, Han DW, Hyon SH, Biomater. Res., 15, 129 (2011)