폴리디메틸실록산 기반 마이크로패턴 채널 시스템을 이용한 단일 세포의 극성 신호에 관한 연구

서정수; 이찬빈; 판이자; 왕잉샤이오; 정영미; 김태진; Jung-Soo Suh; Chanbin Lee; Yijia Pan; Yingxiao Wang; Youngmi Jung; Tae-Jin Kim

Korean Chemical Engineering Research, Vol.58, No.1, 122-126, February, 2020

DOI10.9713/kcer.2020.58.1.122 Export Citation

폴리디메틸실록산 기반 마이크로패턴 채널 시스템을 이용한 단일 세포의 극성 신호에 관한 연구

A Study on Single Cell Polarized Signals Using Polydimethylsiloxane-based Micropatterned Channel System

서정수¹, 이찬빈¹, 판이자², 왕잉샤이오², 정영미^{1, 3}, 김태진^{1, 3, †}

¹부산대학교 생명시스템학과, 46241 부산광역시 금정구 부산대학로63번길 2
²캘리포니아 샌디에고 대학교 공과대학 생명공학과, 92093 USA 캘리포니아 라 호야 9500길만길
³부산대학교 생명과학과, 46241 부산광역시 금정구 부산대학로63번길 2

Jung-Soo Suh¹, Chanbin Lee¹, Yijia Pan², Yingxiao Wang², Youngmi Jung^{1, 3}, and Tae-Jin Kim^{1, 3, †}

¹Department of Integrated Biological Science, Pusan National University, 2, Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 46241, Korea
²Department of Bioengineering, University of California at San Diego, La Jolla, CA 92093, USA
³Department of Biological Sciences, Pusan Nationl University, 2, Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 46241, Korea

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초록

본 연구에서는 폴리디메틸실록산(PDMS)과 모세관-미세몰딩(MIMIC) 기술을 활용하여 마이크로패턴 채널 시스템을 제작하고, 단일 세포 수준에서 극성화 패턴으로 형성되는 분자 신호를 고해상도 세포 이미징을 통해 분석하였다. 이 과정에서 혈소판유래성장인자(PDGF)가 처리된 세포에서는 세포 이동에 중요한 세 종류의 신호인 포스포이노시티드 3-인산화효소(PI3K), Rac 및 액틴(Actin) 신호가 선두(front)영역에서 후미(rear)영역에 비해 강하게 활성화 하는 데 반해, 마이오신 경쇄(MLC) 신호는 비특이적 경향성을 보여주었다. 본 연구 결과는 향후 마이크로패턴의 미세환경에서 세포 극성화 신호와 세포 이동과의 상관 관계를 연구하는 데 중요한 도움이 될 것으로 사료된다.

In this study, we produced the micropatterned channel system using polydimethylsiloxane (PDMS) and micromolding in capillaries (MIMIC) technology and evaluated cellular polarity signals through high-resolved imaging at the single-cell level. In cells treated with platelet-derived growth factor (PDGF), three types of key signals in cell migration; phosphoinositide 3-kinase (PI3 K), Rac, and Actin, were strongly activated in the front area compared to the rear region, whereas myosin light chain (MLC) showed no notable activity in the front and rear areas. Our results will, therefore, provide important information and methodology for studying the correlation between cell polarity signals and cell migration under the newly defined microenvironment.

Keywords:Polydimethylsiloxane;Micromolding in capillaries;Micropattern;Cell polarity;Cell imaging

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[7] McLennan R, Dyson L, Prather KW, Morrison JA, Baker RE, Maini PK, Kulesa PM, Development, 139(16), 2935 (2012)

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