Impregnation of probiotics into porous TiO2 support for enhanced viability

Ju-yeon Byeon; Dasom Park; Tae Wan Kim; Chang Hyun Ko; Jong-il Choi

Korean Journal of Chemical Engineering, Vol.38, No.3, 475-479, March, 2021

DOI10.1007/s11814-020-0728-6 Export Citation

Impregnation of probiotics into porous TiO2 support for enhanced viability

Ju-yeon Byeon, Dasom Park¹, Tae Wan Kim, Chang Hyun Ko^{1, †}, and Jong-il Choi^†

Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju 61186, Korea
¹School of Chemical Engineering, Chonnam National University, Gwangju 61186, Korea

E-mail:,

The viability of probiotics in titania (TiO2) support was assessed in simulated gastrointestinal environment. TiO2 support with macropores was synthesized using titanium (IV) isopropoxide (TTIP) as a precursor and impregnated with probiotics including Lactobacillus paracasei and Streptococcus salivarius, respectively. Scanning electron microscopy analysis after impregnation with probiotics revealed that the probiotics were located inside the macropores of the support. Compared with non-impregnated free probiotics, the impregnated probiotics survived at a higher rate in a simulated gastrointestinal environment. The probiotics impregnated in the TiO2 support exhibited low viability in the simulated stomach environment, but their viability recovered in the simulated intestinal environment. However, free probiotics did not exhibit any recovery of viability under the same conditions. These results suggest that the TiO2 support enhanced the stability of the impregnated probiotics against environmental stress in the gastrointestinal tract.

Keywords:Probiotics;Titanium Dioxide;Impregnation;Gastrointestinal Conditions

[References]

Reid G, Jass J, Sebulsky MT, McCormick JK, Clin. Microbiol. Rev, 16, 658 (2003)
Rokka S, Rantamaki P, Eur. Food Res. Technol., 231, 1 (2010)
Ma B, Forney LJ, Ravel J, Annu. Rev. Microbiol., 66, 371 (2012)
Ayala G, Escobedo-Hinojosa WI, de La Cruz-Herrera CF, Romero I, World J. Gastroenterol., 20, 1450 (2014)
Rault A, Bouix M, Beal C, Appl. Environ. Microbiol., 75, 4374 (2009)
Lu PJ, Hsu PI, Chen CH, Hsiao M, Chang WC, Tseng HH, Lin KH, Chuah SK, Chen HC, World J. Gastroenterol., 16, 5496 (2010)
Crapisi A, Lante A, Pasini G, Spettoli P, Process Biochem., 28, 17 (1993)
Buck LM, Gilliland S, J. Dairy Sci., 77, 2925 (1994)
Lee Y, Ji YR, Lee S, Choi MJ, Cho Y, J. Microbiol. Biotechnol., 29, 721 (2019)
Jarudilokkul S, Tongthammachat A, Boonamnuayvittaya V, Korean J. Chem. Eng., 28, 1247 (2001)
Wang Z, Li X, Cui Y, Cheng K, Dong M, Liu L, Korean J. Chem. Eng., 37(10), 1732 (2020)
Chantawongvuti R, Veerajetbodithat J, Jaturapiree P, Muangnapoh C, J. Microbiol. Biotechnol., 20, 110 (2010)
Jewell S, Kimball SM, US Geological Survey, 9, 196 (2015)
Yang Y, Doudrick K, Bi X, Hristovski K, Herckes P, Westerhoff P, Kaegi R, Environ. Sci. Technol., 11, 6391 (2014)
Cheng H, Ma J, Zhao Z, Qi L, Chem. Mater., 7, 663 (1995)
Puma GL, Bono A, Krishnaiah D, Collin JG, J. Hazard. Mater., 157(2-3), 209 (2008)
Yu JG, Su YR, Cheng B, Adv. Funct. Mater., 17(12), 1984 (2007)
Yu J, Zhang L, Cheng B, Su Y, J. Phys. Chem. C., 111, 10582 (2007)
Oomen A, Rompelberg C, Bruil M, Dobbe C, Pereboom D, Sips A, Arch. Environ. Contam. Toxicol., 44, 0281 (2003)
Minekus M, Alminger M, Alvito P, Ballance S, Bohn T, Bourlieu C, Carriere F, Boutrou R, Corredig M, Dupont D, Food Funct., 5, 1113 (2014)
Yamashita C, Chung MMS, dos Santos C, Mayer CRM, Moraes ICF, Branco IG, LWT-Food Sci. Technol., 84, 256 (2017)
Collins MD, Phillips BA, Zanoni P, Int. J. Syst. Evol. Microbiol., 39, 105 (1989)
Kokkinosa A, Fasseas C, Eliopoulos E, Kalantzopoulos G, Le Lait., 78, 491 (1998)
O’Sullivan TF, Fitzgerald GF, J. Appl. Microbiol., 86, 275 (1999)
Correa NB, Peret Filho LA, Penna FJ, Lima FM, Nicoli JR, J. Clin. Gastroenterol., 39, 385 (2005)
Beal C, Fonseca F, Corrieu G, J. Dairy Sci., 84, 2347 (2001)
Christensen F, Davis S, Hardy J, Taylor M, Whalley D, Wilson C, J. Pharm. Pharmacol., 37, 91 (1985)
Kim H, Cult. Dairy Prod. J., 24, 6 (1988)

[1] Reid G, Jass J, Sebulsky MT, McCormick JK, Clin. Microbiol. Rev, 16, 658 (2003)

[2] Rokka S, Rantamaki P, Eur. Food Res. Technol., 231, 1 (2010)

[3] Ma B, Forney LJ, Ravel J, Annu. Rev. Microbiol., 66, 371 (2012)

[4] Ayala G, Escobedo-Hinojosa WI, de La Cruz-Herrera CF, Romero I, World J. Gastroenterol., 20, 1450 (2014)

[5] Rault A, Bouix M, Beal C, Appl. Environ. Microbiol., 75, 4374 (2009)

[6] Lu PJ, Hsu PI, Chen CH, Hsiao M, Chang WC, Tseng HH, Lin KH, Chuah SK, Chen HC, World J. Gastroenterol., 16, 5496 (2010)

[7] Crapisi A, Lante A, Pasini G, Spettoli P, Process Biochem., 28, 17 (1993)

[8] Buck LM, Gilliland S, J. Dairy Sci., 77, 2925 (1994)

[9] Lee Y, Ji YR, Lee S, Choi MJ, Cho Y, J. Microbiol. Biotechnol., 29, 721 (2019)

[10] Jarudilokkul S, Tongthammachat A, Boonamnuayvittaya V, Korean J. Chem. Eng., 28, 1247 (2001)

[11] Wang Z, Li X, Cui Y, Cheng K, Dong M, Liu L, Korean J. Chem. Eng., 37(10), 1732 (2020)

[12] Chantawongvuti R, Veerajetbodithat J, Jaturapiree P, Muangnapoh C, J. Microbiol. Biotechnol., 20, 110 (2010)

[13] Jewell S, Kimball SM, US Geological Survey, 9, 196 (2015)

[14] Yang Y, Doudrick K, Bi X, Hristovski K, Herckes P, Westerhoff P, Kaegi R, Environ. Sci. Technol., 11, 6391 (2014)

[15] Cheng H, Ma J, Zhao Z, Qi L, Chem. Mater., 7, 663 (1995)

[16] Puma GL, Bono A, Krishnaiah D, Collin JG, J. Hazard. Mater., 157(2-3), 209 (2008)

[17] Yu JG, Su YR, Cheng B, Adv. Funct. Mater., 17(12), 1984 (2007)

[18] Yu J, Zhang L, Cheng B, Su Y, J. Phys. Chem. C., 111, 10582 (2007)

[19] Oomen A, Rompelberg C, Bruil M, Dobbe C, Pereboom D, Sips A, Arch. Environ. Contam. Toxicol., 44, 0281 (2003)

[20] Minekus M, Alminger M, Alvito P, Ballance S, Bohn T, Bourlieu C, Carriere F, Boutrou R, Corredig M, Dupont D, Food Funct., 5, 1113 (2014)

[21] Yamashita C, Chung MMS, dos Santos C, Mayer CRM, Moraes ICF, Branco IG, LWT-Food Sci. Technol., 84, 256 (2017)

[22] Collins MD, Phillips BA, Zanoni P, Int. J. Syst. Evol. Microbiol., 39, 105 (1989)

[23] Kokkinosa A, Fasseas C, Eliopoulos E, Kalantzopoulos G, Le Lait., 78, 491 (1998)

[24] O’Sullivan TF, Fitzgerald GF, J. Appl. Microbiol., 86, 275 (1999)

[25] Correa NB, Peret Filho LA, Penna FJ, Lima FM, Nicoli JR, J. Clin. Gastroenterol., 39, 385 (2005)

[26] Beal C, Fonseca F, Corrieu G, J. Dairy Sci., 84, 2347 (2001)

[27] Christensen F, Davis S, Hardy J, Taylor M, Whalley D, Wilson C, J. Pharm. Pharmacol., 37, 91 (1985)

[28] Kim H, Cult. Dairy Prod. J., 24, 6 (1988)