In this work, the combination of different lipases was employed for the production of biodiesel, using the Free Fatty Acids (FFAs) from Residual Chicken Oil (RCO) as a substrate. As biocatalysts, lipases A and B fromCandida antarctica(CALA and CALB, respectively) and lipase fromRhizomucor miehei(RML) were used in different combinations; as a result, the best lipase cocktail was composed of 67% CALB and 33% RML, which was used to optimize the esterification reaction parameters (molar ratio (FFAs/ ethyl alcohol), biocatalyst content, temperature and time) by the Taguchi method. Under optimized conditions (1:5, 15% of lipase cocktail, 30 degrees C and 3 h), it was possible to achieve 89.95 +/- 0.3% of conversion. [GRAPHICS] .