Gentamicin/CTMA/Montmorillonite as Slow-Released Antibacterial Agent

Is Fatimah; Habibi Hidayat; Gani Purwiandono; Saddam Husein; Won-Chun Oh

Korean Journal of Materials Research, Vol.31, No.6, 367-374, June, 2021

DOI10.3740/MRSK.2021.31.6.367 Export Citation

Gentamicin/CTMA/Montmorillonite as Slow-Released Antibacterial Agent

Is Fatimah^†, Habibi Hidayat, Gani Purwiandono, Saddam Husein¹, and Won-Chun Oh²

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia, Kampus Terpadu UII, Jl. Kaliurang Km 14, Sleman, Yogyakarta, Indonesia
¹Laboratory of Materials for Energy and Environemnt, Department of Chemistry, Universitas Islam Indonesia, Kampus Terpadu UII, Jl. Kaliurang Km 14, Sleman, Yogyakarta, Indonesia
²Department of Advanced Materials Science and Engineering, Hanseo University, Seosan-si, Chungnam 356-706, South Korea

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This paper presents the characteristics of gentamicin-loaded into cetyl trimethyl ammonium intercalated montmorillonite (GtM/CTMA/Mt) as a hybrid composite for a slow-released antibacterial delivery systems. The work describes the successful immobilization of gentamicin into the interlayers of surfactant-modified montmorillonite. Physicochemical characterization of the material is carried out by means of X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and Fourier transform infrared spectroscopy. The kinetics of the gentamicin release is investigated by in vitro study and analyzed based on UV-Vis spectrometry. In addition, antibacterial study is performed towards Klebsiella pneumoniae Staphylococcus aureus, Escherichia coli, and Streptococcus pyogenes. The results show that the gentamicin loading into CTMA/ Mt increases the effectiveness of the antibacterial activity, as shown by the higher inhibition zone for all tested bacteria, compared to gentamicin as a positive control. The kinetics study suggests that the gentamicin release obeys the modified Korsmeyer-Peppas model. The physicochemical study and activity test demonstrate the feasibility of the GtM/CTMA/Mt for practical applications.

Keywords:antibacterial agent;slow-released drug;intercalated clay;organoclay

[References]

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[1] Gao P, Nie X, Zou M, Shi Y, Cheng G, J. Antibiot., 64, 625 (2011)

[2] He Y, Wu Z, Tu L, Han Y, Zhang G, Li C, Appl. Clay Sci., 109-110, 68 (2015)

[3] Bujdakova H, Bujdakova V, Majekova-Koscova H, Gaalova B, Bizovska V, Bohac P, Bujdak J, Appl. Clay Sci., 158, 21 (2018)

[4] Anggraini M, Kurniawan A, Ong LK, Martin MA, Liu JC, Soetaredjo FE, Indraswati N, Ismadji S, RSC Adv., 4, 16298 (2014)

[5] Saitoh T, Shibayama T, J. Hazard. Mater., 317, 677 (2016)

[6] Herrera P, Burghardt RC, Phillips TD, Vet. Microbiol., 74, 259 (2000)

[7] Virto MR, Frutos P, Torrado S, Frutos G, Biomaterials, 24, 79 (2003)

[8] Perni S, Martini-Gilching K, Prokopovich P, Colloids Surf. A: Physicochem. Eng. Asp., 541, 212 (2018)

[9] Stevanovic M, Dosic M, Jankovic A, Kojic V, et al., ACS Biomater. Sci. Eng., 4, 3994 (2018)

[10] Tang S, Tian B, Ke QF, Zhu ZA, Guo YP, RSC Adv., 40, 41500 (2014)

[11] Mosselhy DA, Ge Y, Gasik M, Nordstrom K, Natri O, Hannula SP, Materials, 9, 1 (2016)

[12] Zarate-Reyes L, Lopez-Pacheco C, Nieto-Camacho A, Palacios E, Gomez-Vidales V, Kaufhold S, Ufer K, Zepeda EG, Cervini-Silva J, J. Hazard. Mater., 342, 625 (2018)

[13] Maryan AS, Montazer M, Rashidi A, Rahimi MK, Asian J. Chem., 25, 2889 (2013)

[14] Lv G, Pearce CW, Gleason A, Liao L, MacWilliams MP, Li Z, J. Asian Earth Sci., 77, 281 (2013)

[15] Zhang L, Chen J, Yu W, Zhao Q, Liu J, J. Nanomater., 2018, 619025 (2018)

[16] Yuan X, Zhang J, Zhang R, Liu j, Wang W, Hou H, Materials, 12, 4148 (2019)

[17] Honarmand M, Golmohammadi M, Naeimi A, Mater. Chem. Phys., 241, 122416 (2020)

[18] Fatimah I, Rubiyanto D, Sahroni I, Putra RS, Nurillahi R, Nugraha J, Appl. Clay Sci., 193, 105671 (2020)

[19] Fatimah I, Huda T, Appl. Clay Sci., 74, 115 (2013)

[20] Luo W, Geng Z, Li Z, Wu S, Cui Z, Zhu S, Liang Y, Yang X, Int. J. Nanomedicine, 13, 7491 (2018)

[21] Batul R, Bhave M, Mahon PJ, Yu A, Molecules, 25, 1 (2020)

[22] Bayoumi A, Sarg MT, Fahmy TYA, Mohamed NF, El-Zawawy WK, Arab. J. Chem., 13, 8920 (2020)

[23] Rapacz-Kmita A, Stodolak-Zych E, Dudek M, Gajek M, Ziabka M, J. Therm. Anal. Calorim., 127, 871 (2017)

[24] Liu Y, Ji P, Lv H, Qin Y, Deng L, Int. J. Biol. Macromol., 98, 550 (2017)

[25] Karthikeyan M, Deepa MK, Bassim E, Rahna CS, Raj KRS, J. Pharm. Innov., Research Article (2020).

[26] Wojcik-Pastuszka D, Krzak J, Macikowski B, Berkowski R, Osinski B, Materials, 12, 1202 (2019)

[27] Wu IY, Bala S, Skalko-Basnet N, Cagno MPD, Eur. J. Pharm. Sci., 138, 105026 (2019)

[28] Arora G, Malik K, Singh I, Polim. Med., 41, 23 (2011)

[29] Gouda R, Baishya H, Qing Z, J. Dev. Drugs., 06, 100017 (2017)

[30] Chen X, Xu C, He H, Biophys. Res. Commun., 516, 1085 (2019)

[31] Posadowska U, Brzychczy-Wloch M, Acta Bioeng. Biomech., 17, 41 (2015)

[32] Stigter M, Bezemer J, Groot KD, Layrolle P, J. Control. Release, 99, 127 (2004)

[33] Franci G, Falanga A, Galdiero S, Palomba L, Rai M, Morelliiano G, Galdiero M, Molecules, 20, 8856 (2015)

[34] Garibo D, Borbon-Nunez HA, Leon JNDD, et al., Sci. Rep., 10, 12805 (2020)

[35] Loo YY, Rukayadi Y, Nor-Khaizura MAR, Kuan CH, Chieng BW, Nishibuchi M, Radu S, Front. Microbiol., 9, 1 (2018)