화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.107, 155-164, March, 2022
DOI10.1016/j.jiec.2021.11.044  Export Citation
Intestinal extracellular matrix hydrogels to generate intestinal organoids for translational applications
Suran Kim1, Yi Sun Choi1, Jung Seung Lee1, 2, Sung-Hyun Jo3, Yun-Gon Kim3, and Seung-Woo Cho1, 4, †
  • 1Department of Biotechnology, Yonsei University, Seoul 03722, Republic of Korea
  • 2Department of Intelligent Precision Healthcare Convergence, Sungkyunkwan University, Suwon 16419, Republic of Korea
  • 3Department of Chemical Engineering, Soongsil University, Seoul 06978, Republic of Korea
  • 4Center for Nanomedicine, Institute for Basic Science (IBS), Seoul 03722, Republic of Korea
E-mail:
Intestinal organoids are emerging tools in intestinal disease modeling and regeneration. Matrigel, a basement membrane matrix extracted from mouse sarcoma, is the gold standard material for intestinal organoid culture. Yet, the tumor origin of this matrix does not effectively provide organoids with native intestine-like microenvironments. To overcome this limitation, we demonstrate a decellularized small intestine-derived hydrogel reinforced with three extracellular matrix (ECM) proteins that are essential for intestinal development. To identify optimal conditions for intestinal organoid culture, we optimized the concentrations of the ECM hydrogel derived from decellularized intestine and three ECM proteins. The intestinal organoids grown in the established ECM hydrogels have shown similarity to those grown in Matrigel in terms of organoid forming efficiency, size, and gene expression patterns of intestine-specific markers. Our intestine-mimetic hydrogel could increase the feasibility of intestinal organoids for more clinically relevant applications in disease modeling and regenerative medicine.
Keywords:Decellularization;Intestinal extracellular matrix;Intestinal organoid;Hydrogel
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