화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.108, 159-169, April, 2022
DOI10.1016/j.jiec.2021.12.038  Export Citation
Pectin nanogel formation via thiol-norbornene photo-click chemistry for transcutaneous antigen delivery
Siyeong Lee1, Changhee Woo1, and Chang Seok Ki1, 2, †
  • 1Department of Agriculture, Forestry and Bioresources, Seoul National University, Seoul 08826, Republic of Korea
  • 2Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Republic of Korea
E-mail:
Pectin nanogels were fabricated with norbornene group functionalized pectin, dithiol crosslinker, and thiolated ovalbumin (OVA) via a thiol-norbornene photo-click reaction and ultrasonication to develop a novel transcutaneous antigen-delivery carrier. In bulk gel characterization, the physical properties and OVA-loading efficiency were modulated by controlling the crosslinking density and stoichiometric balance between thiol and norbornene groups. Pectin nanogels were of uniform size (~200 nm in diameter) and quite stable under storage conditions (4 ºC). The OVA-loaded pectin nanogels penetrated the stratum corneum and were deposited in both the epidermis and dermis, while soluble OVA did not penetrate the stratum corneum layer. The nanogels were internalized by dendritic cells derived from THP-1 monocytes, inducing the upregulation of maturation markers. These results indicate that pectin nanogels are promising carriers for transcutaneous antigen delivery.
Keywords:Pectin;Nanogel;Transcutaneous delivery;Dendritic cell
  1. Zhao Z, Ukidve A, Dasgupta A, Mitragotri S, Adv. Drug Deliv. Rev., 127, 3 (2018)
  2. Bolzinger MA, Briançon S, Pelletier J, Chevalier Y, Curr. Opin. Colloid Interface Sci., 17(3), 156 (2012)
  3. Gamazo C, Pastor Y, Larrañeta E, Berzosa M, Irache JM, Donnelly RF, Ther. Deliv., 10(1), 63 (2019)
  4. Feng X, Xu W, Li Z, Song W, Ding J, Chen X, Adv. Sci., 6(17), 1900101 (2019)
  5. Lee MY, Shin MC, Yang VC, BMB Rep., 46(1), 17 (2013)
  6. Szczepanik M, Majewska-Szczepanik M, Pharmacol. Rep., 68(4), 773 (2016)
  7. Bäsler K, Bergmann S, Heisig M, Naegel A, Zorn-Kruppa M, Brandner JM, J. Control Release, 242, 105 (2016)
  8. Carter P, Narasimhan B, Wang Q, Int. J. Pharm., 555, 49 (2019)
  9. Roberts MS, Mohammed Y, Pastore MN, Namjoshi S, Yousef S, Alinaghi A, et al., J. Control. Release, 247, 86 (2017)
  10. Chacko IA, Ghate VM, Dsouza L, Lewis SA, Colloids Surf. B: Biointerfaces, 195, 111262 (2020)
  11. Park SN, Jo NR, Jeon SH, J. Ind. Eng. Chem., 20(4), 1481 (2014)
  12. Hua S, Front. Pharmacol., 6, 219 (2015)
  13. Benson HA, Expert Opin. Drug Deliv., 3(6), 727 (2006)
  14. Tyagi RK, Garg NK, Jadon R, Sahu T, Katare OP, Dalai SK, et al., Vaccine, 33(36), 4630 (2015)
  15. Chaudhari R, Tandel N, Sahu K, Negi S, Bashir H, Rupareliya A, Mishra RPN, Dalai SK, Tyagi RK, Nanomaterials, 11, 406 (2021)
  16. Mishra V, Bansal K, Verma A, Yadav N, Thakur S, Sudhakar K, et al., Pharmaceutics, 10(4), 191 (2018)
  17. Kwon TK, Lim KB, Kim JC, J. Ind. Eng. Chem., 17(1), 10 (2011)
  18. Toyoda M, Hama S, Ikeda Y, Nagasaki Y, Kogure K, Int. J. Pharm., 483(1-2), 110 (2015)
  19. Li D, Sun F, Bourajjaj M, Chen Y, Pieters EH, Chen J, et al., Nanoscale, 8(47), 19592 (2016)
  20. Nochi T, Yuki Y, Takahashi H, Sawada SI, Mejima M, Kohda T, et al., Nat. Mater., 9(7), 572 (2010)
  21. Lin CC, Ki CS, Shih H, J. Appl. Polym. Sci., 132(8) (2015)
  22. Shih H, Lin CC, Biomacromolecules, 16(7), 1915 (2015)
  23. Bussio JI, Molina-Perea C, González-Aramundiz JV, Pharmaceutics, 11(5), 246 (2019)
  24. Kim KS, Kim H, Park Y, Kong WH, Lee SW, Kwok SJJ, et al., Adv. Funct. Mater., 26(15), 2512 (2016)
  25. Kim H, Lee S, Ki CS, Carbohydr. Polym., 252, 117132 (2021)
  26. Fallacara A, Baldini E, Manfredini S, Vertuani S, Polymer, 10(7), 701 (2018)
  27. Jung HS, Kim KS, Yun SH, Hahn SK, Nanomedicine, 9(6), 743 (2014)
  28. Hanauer DC, de Souza AG, Cargnin MA, Gasparin BC, Rosa DS, Paulino AT, J. Ind. Eng. Chem., 97, 368 (2021)
  29. Liang RH, Wang LH, Chen J, Liu W, Liu CM, Food Hydrocolloids, 50, 65 (2015)
  30. Ishisono K, Yabe T, Kitaguchi K, J. Nutr. Biochem., 50, 38 (2017)
  31. Morris VJ, Belshaw NJ, Waldron KW, Maxwell EG, Bioact. Carbohydr. Diet. Fibre, 1(1), 21 (2013)
  32. Prado SB, Beukema M, Jermendi E, Schols HA, de Vos P, Fabi JP, Sci. Rep., 10(1), 1 (2020)
  33. Berges C, Naujokat C, Tinapp S, Wieczorek H, Höh A, Sadeghi M, et al., Biochem. Biophys. Res. Commun., 333(3), 896 (2005)
  34. Li N, Wang H, Qu X, Chen Y, Mar. Drugs, 15(7), 223 (2017)
  35. Pereira RF, Barrias CC, Bártolo PJ, Granja PL, Acta Biomater., 66, 282 (2018)
  36. Wang Y, Rapakousiou A, Astruc D, Macromolecules, 47(12), 3767 (2014)
  37. Ishak KMK, Ahmad Z, Akil HM, e-Polymers, 10(1), 64 (2010)
  38. Light TS, Anal. Chem., 56(7), 1138 (1984)
  39. Van Thienen TG, Lucas B, Demeester J, De Smedt SC, J. Control. Release, 116(2), e12 (2006)
  40. Wooster TJ, Acquistapace S, Mettraux C, Donato L, Dekkers BL, J. Colloid Interface Sci., 553, 308 (2019)
  41. Salatin S, Khosroushahi AY, J. Cell. Mol. Med., 21(9), 1668 (2017)
  42. Aerts-Toegaert C, Heirman C, Tuyaerts S, Corthals J, Aerts J, Bonehill A, et al., Eur. J. Immunol., 37(3), 686 (2007)
  43. Lechmann M, Zinser E, Golka A, Steinkasserer A, Int. Arch. Allergy Immunol., 129(2), 113 (2002)
  44. Li Z, Ju X, Silveira PA, Abadir E, Hsu WH, Hart DN, et al., Front. Immunol., 10, 1312 (2019)
  45. Van Niel G, Wubbolts R, Stoorvogel W, Curr. Opin. Cell Biol., 20(4), 437 (2008)
  46. Gardner A, Ruffell B, Trends Immunol., 37(12), 855 (2016)
  47. Grosche L, Knippertz I, König C, Royzman D, Wild AB, Zinser E, et al., Front. Immunol., 11 (2020)