Effect of Packing Size on Chromatographic Separation of Catechin Compounds in Green Tea

Ji Hoon Kang; Seung Bum Hong; Jung Il Kim; Sung Taik Chung; Kyung Ho Row

Korean Journal of Chemical Engineering, Vol.17, No.6, 723-726, November, 2000

DOI10.1007/BF02699124 Export Citation

Effect of Packing Size on Chromatographic Separation of Catechin Compounds in Green Tea

Ji Hoon Kang, Seung Bum Hong, Jung Il Kim, Sung Taik Chung, and Kyung Ho Row^†

Center of Advanced Bioseparation Technology and Division of Chemical Engineering, Inha University, Inchon 402-751, Korea

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EGCG (Epigallocatechin Gallate), one of the catechin compounds abundant mainly in green tea, has chemopreventive effects against carcinogenesis. The extract at 50 ℃ water from the powder of green tea was partitioned with chloroform and ethyl acetate. The resulting solution was further purified on three different columns, 4.6x250 mm (40/63 μm), 4.6x250 mm (15 μm), 3.9x300 mm (10 μm), in order to separate EGCG among catechin compounds from green tea. In a given packing size, the composition of the binary mobile phase, water and acetonitrile, with 0.1% acetic acid was changed to operate in optimized experimental conditions. In a packed column of 40/63 μm packing, catechin compounds were not well separated. With packing sizes of 15 and 10 μm, the resolution of EGCG among catechin compounds was better, and maximum injection volume to separate EGCG purely was 100 and 480 μl, respectively. The study on the effect of packing size may be utilized for preparative chromatographic separation.

Keywords:Green Tea;Epigallocatechin Gallate;Packing Size;Chromatography

[References]

Ahmad N, Feyes DK, Nieminen AL, Agarwal R, Mukhtar H, J. National Cancer Institute, 89(24), 1881 (1997)
Bronner WE, Beecher GR, J. Chromatogr. A, 805, 137 (1998)
Choi SH, Kim SH, Lee BH, J. Korean Soc. Food Nutr., 22, 374 (1993)
Chung ST, Row KH, Kang JH, Park YK, pending to Korean patent, 42877 (1998)
Goto T, Yoshida Y, Kiso M, Nagashima H, J. Chromatogr. A, 749, 295 (1996)
Jung YA, Row KH, Korean J. Chem. Eng., 15(5), 538 (1998)
Kang JH, Park YK, Chung ST, Row KH, Korean J. Biotechnol. Bioeng., 14(5), 517 (1999)
Kulik A, Fiedler HP, J. Chromatogr. A, 812, 117 (1998)
Lee JH, Lee YM, Moon DC, J. Korean Soc. Anal. Sci., 5(3), 333 (1992)
Row KH, "Principles and Applications of Liquid Chromatography," Inha Univ. Press, Inchon (1999)
Row KH, Lee JW, Korean J. Chem. Eng., 14(5), 412 (1997)
Row KH, Lee YY, Korean J. Chem. Eng., 9(2), 83 (1992)
Santosh K, Rajesh A, Susan E, Gary SW, Hasan M, Carcinogenesis, 14, 361 (1993)
Valcic S, Timmermann BN, Alberts DS, Wachter GA, Krutzsch M, Wymer J, Guillen JM, Anti-Cancer Drugs, 7, 461 (1996)
Yamane T, Hagiwara N, Tateichi M, Akachi S, Kim M, Okuzumi J, Kitao Y, Inagake M, Kuwata K, Takahachi T, Jpn. J. Cancer Res., 82, 1336 (1991)

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[Related Articles]

[1] Ahmad N, Feyes DK, Nieminen AL, Agarwal R, Mukhtar H, J. National Cancer Institute, 89(24), 1881 (1997)

[2] Bronner WE, Beecher GR, J. Chromatogr. A, 805, 137 (1998)

[3] Choi SH, Kim SH, Lee BH, J. Korean Soc. Food Nutr., 22, 374 (1993)

[4] Chung ST, Row KH, Kang JH, Park YK, pending to Korean patent, 42877 (1998)

[5] Goto T, Yoshida Y, Kiso M, Nagashima H, J. Chromatogr. A, 749, 295 (1996)

[6] Jung YA, Row KH, Korean J. Chem. Eng., 15(5), 538 (1998)

[7] Kang JH, Park YK, Chung ST, Row KH, Korean J. Biotechnol. Bioeng., 14(5), 517 (1999)

[8] Kulik A, Fiedler HP, J. Chromatogr. A, 812, 117 (1998)

[9] Lee JH, Lee YM, Moon DC, J. Korean Soc. Anal. Sci., 5(3), 333 (1992)

[10] Row KH, "Principles and Applications of Liquid Chromatography," Inha Univ. Press, Inchon (1999)

[11] Row KH, Lee JW, Korean J. Chem. Eng., 14(5), 412 (1997)

[12] Row KH, Lee YY, Korean J. Chem. Eng., 9(2), 83 (1992)

[13] Santosh K, Rajesh A, Susan E, Gary SW, Hasan M, Carcinogenesis, 14, 361 (1993)

[14] Valcic S, Timmermann BN, Alberts DS, Wachter GA, Krutzsch M, Wymer J, Guillen JM, Anti-Cancer Drugs, 7, 461 (1996)

[15] Yamane T, Hagiwara N, Tateichi M, Akachi S, Kim M, Okuzumi J, Kitao Y, Inagake M, Kuwata K, Takahachi T, Jpn. J. Cancer Res., 82, 1336 (1991)