Bioresource Technology, Vol.53, No.3, 269-275, 1995
On the kinetics and effectiveness of immobilized whole-cell batch cultures
A model for growth-associated fermentations is presented for batch cultures of immobilized whole cells. The model encompasses exponential and steady-state growth phases and the effectiveness of the biocatalyst. Based on the model equations, experimental methods have been developed for the determination of the kinetic parameters: maximum specific growth rate (mu(max)), Monod constant (K-m) and effectiveness factor (eta). These methods are illustrated by ethanol fermentations using Saccharomyces cerevisiae cells entrapped in alginate beads. The kinetic parameters obtained show consistency with those determined for free-cell systems.
Keywords:immobilized whole cells;effectiveness factor;biomass yield factor;exponential growth phase;steady-state growth phase;Gaden type I fermentations