Analysis of Microbial Adaptation at Enzyme Level for Enhancing Biodegradation Rate of BTX

Sung-Ho Yeom; Young-Je Yoo

Korean Journal of Chemical Engineering, Vol.19, No.5, 780-782, September, 2002

DOI10.1007/BF02706967 Export Citation

Analysis of Microbial Adaptation at Enzyme Level for Enhancing Biodegradation Rate of BTX

Sung-Ho Yeom^{1, 2}, and Young-Je Yoo^{1, †}

¹School of Chemical Engineering, Seoul National University, Seoul 151-742, Korea
²EnzyBiotech Inc., Technology Incubation Network, Seoul National University, Seoul 151-742, Korea

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Catechol was found to be a common intermediate in the degradation of benzene and toluene by Alcaligenes xylosoxidans Y234, and the ring cleavage of the catechol mediated by catechol 1,2-dioxygenase was a rate-determining step. Since benzene induced higher level of catechol 1,2-dioxygenase than toluene, the cells pre-adapted to benzene showed higher degradation rate of benzene and toluene. The degradation rate of m-xylene was also increased significantly when benzene-adapted cells were inoculated. m-Xylene was metabolized via 3-methyl catechol which was effectively cleaved by catechol 1,2-dioxygenase.

Keywords:BTX;Microbial Adaptation;Enzyme Induction;Catechol 1,2-Dioxygenase;Biodegradation

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[Referenced By]

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[1] Arnow LE, J. Biol. Chem., 118, 531 (1937)

[2] Bauer JE, Capone DG, Appl. Environ. Microbiol., 54, 1649 (1988)

[3] Duetz WA, deJong C, Williams PA, vanAndel JG, Appl. Environ. Microbiol., 60, 2858 (1994)

[4] Evans WC, J. Biochem., 41, 373 (1947)

[5] Folsorn BR, Chapman PJ, Pritchard PH, Appl. Environ. Microbiol., 56, 1279 (1990)

[6] Fujita M, Ike M, Kamiya T, Water Res., 27, 91 (1993)

[7] Hamzah RY, Albaharna BS, Appl. Microbiol. Biotechnol., 41(2), 250 (1994)

[8] Heipieper HJ, deBont JAM, Appl. Environ. Microbiol., 60, 4440 (1994)

[9] Satsangee R, Ghosh P, Appl. Microbiol. Biotechnol., 34, 127 (1990)

[10] Shimp RJ, Pfaender FK, Appl. Environ. Microbiol., 49, 394 (1985)

[11] Waite JH, Tanzer ML, Anal. Biochem., 111, 131 (1981)

[12] Yeom SH, Kim SH, Yoo YJ, Korean J. Chem. Eng., 14(1), 37 (1997)

[13] Yeom SH, Lee JH, Yoo YJ, J. Microbiol. Biotechnol., 8, 222 (1998)