화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Journal of Chemical Engineering, Vol.20, No.3, 549-553, May, 2003
DOI10.1007/BF02705564  Export Citation
Kinetic Model for the Simulation of Hen Egg White Lysozyme Adsorption at Solid/Water Interface
Woo-Kul Lee1, 2, and Jea Seung Ko1, †
  • 1Laboratory for the Study of Molecular Biointerfaces, Intellectual Biointerface Engineering Center andDepartment of Oral Anatomy, College of Dentistry, BK21HLS, Seoul National University, 28-22, YeonKun-Dong, ChongRo-Ku, Seoul 110-749, Korea
  • 2Department of Chemical Engineering, College of Engineering, Dankook University, Hannam-Dong, Yongsan-Ku, Seoul 140-714, Korea
E-mail:
A simulation model for adsorption kinetics of hen egg white lysozyme (HEW) adsorption to hydrophilic silica is proposed. The adsorption kinetic data were monitored by using in-situ ellipsometry. The model is based on an irreversible adsorption mechanism allowing two different adsorbed states. The adsorbed states were differentiated based on binding strengths resistant to the concentration gradient exerted by rinse. Molecules desorbing and remaining upon rinse were identified as loosely bound (state 1) and tightly bound (state 2) states, respectively. The adsorption rate constants were assumed to be a time-dependent nonlinear function in order to account for the change in surface properties originating from the protein layer formed on the surface. The parameters of adsorption rate constants were evaluated by using adsorption kinetic data at different protein concentrations, and the relationships between the adsorption parameters and protein concentration were established which eventually demonstrated a linear relationship. The established relations between the adsorption parameters and concentration elucidated the effect of protein concentration on adsorption to hydrophilic silica.
Keywords:Protein Adsorption;Adsorbed State;Lysozyme;Ellipsometry;Kinetic Model
  1. Adamczyk Z, J. Colloid Interface Sci., 229(2), 477 (2000) 
  2. Altankov G, Groth T, J. Mater. Sci. Mater. Med., 5, 732 (1994) 
  3. Andrade JD, "Principles of Protein Adsorption," Surface and Interfacial Aspects of Biomedical Polymers, Andrade, J.D., Ed., Plenum Press, NY (1985)
  4. Arai T, Norde W, Colloids Surf., 51, 1 (1990) 
  5. Beissinger RL, Leonard EF, J. Colloid Interface Sci., 85, 521 (1982) 
  6. Cuypers PA, Corsel JW, Janssen MP, Kop JMM, Hermens WT, Hemker HC, J. Biol. Chem., 258, 2426 (1983)
  7. Elwing H, Welin S, Askendal A, Nilsson U, Lundstrom I, J. Colloid Interface Sci., 119, 203 (1987) 
  8. Horbett TA, J. Biomed. Mater. Res., 15, 673 (1981) 
  9. Imoto T, Johnson LN, North ACT, Philips DC, Rupley JA, "Verbate Lysozyme," The Enzyme, Boyer, P.D., Ed., Academic Press, New York (1972)
  10. Janson JC, Korean J. Chem. Eng., 18(2), 149 (2001)
  11. Kondo A, Oku S, Higashitani K, J. Colloid Interface Sci., 143, 214 (1991) 
  12. Lee WK, Bani-Jaber A, McGuire J, Daeschel MA, Jung IH, Korean J. Chem. Eng., 17(2), 179 (2000)
  13. Lee WK, Ko JS, Kim HM, J. Colloid Interface Sci., 246(1), 70 (2002) 
  14. Lee WK, McGuire J, Bothwell MK, J. Colloid Interface Sci., 213(1), 265 (1999) 
  15. Lu CF, Nadarajah A, Chittur KK, J. Colloid Interface Sci., 168(1), 152 (1994) 
  16. Malmsten M, Colloids Surf. B: Biointerfaces, 3, 297 (1995) 
  17. Matthews BW, Dahlquist FW, Mynard AY, J. Mol. Biol., 78, 575 (1973) 
  18. Mcguire J, Wahlgren MC, Arnebrant T, J. Colloid Interface Sci., 170(1), 182 (1995) 
  19. Norde W, Lyklema J, J. Colloid Interface Sci., 66, 257 (1978) 
  20. Thomas CH, McFarland CD, Jenkins ML, Rezania A, Steele JG, Healy KE, J. Biomed. Mater. Res., 37, 81 (1997) 
  21. Tian MH, Lee WK, Bothwell MK, McGuire J, J. Colloid Interface Sci., 200(1), 146 (1998) 
  22. Tilton RD, Robertson CR, Gast AP, Langmuir, 7, 2710 (1991) 
  23. Wahlgren MC, Paulsson MA, Arnebrant T, Colloids Surf. A: Physicochem. Eng. Asp., 70, 139 (1993) 
  24. Wahlgren M, Arnebrant T, Lundstrom I, J. Colloid Interface Sci., 175(2), 506 (1995) 
  25. Weaver LH, Matthews BW, J. Mol. Biol., 193, 189 (1987) 
  26. Young BR, Pitt WG, Cooper SL, J. Colloid Interface Sci., 124, 28 (1988)