Artificial Dermis Composed of Gelatin, Hyaluronic Acid and (1→3),(1→6)-β-Glucan

Sang Bong Lee; Hyun Wook Jeon; Young Woo Lee; Seong Kwan Cho; Young Moo Lee; Kang Won Song; Moon Hyang Park; Sung Hwa Hong

Macromolecular Research, Vol.11, No.5, 368-374, October, 2003

Sang Bong Lee , Hyun Wook Jeon, Young Woo Lee, Seong Kwan Cho, Young Moo Lee^†, Kang Won Song¹, Moon Hyang Park¹, and Sung Hwa Hong²

School of Chemical Engineering, College of Engineering, Hanyang University, Seoul 133-791, Korea
¹Department of Pathology, College of Medicine, Hanyang University, Seoul 133-791, Korea
²Korea FDA Biologics Evaluation Department, Blood Products Division, Nokburn-Dong 5, Eunpyung-Ku, Seoul 122-070, Korea

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Porous scaffolds composed of gelatin and polysaccharides such as hyaluronic acid and b-glucan were prepared by using the freeze-drying method after cross-linking with 1-ethyl-(3-3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). The scaffold had an inter-connected pore structure with the sufficient pore size for use as a support for the growth of fibroblasts. Results for the contact angle and cell attachment confirmed that high gelatin content in a mixture was suitable for cellular attachment and distribution in two- or three-dimensional fibroblast cultures. However, the addition of polysaccharides aroused the synergistic effects of morphologic and mechanical property of gelatin-based scaffolds. To prepare the artificial dermis for the wound dressing to mimic the normal human dermal skin, fibroblasts were isolated from a childs foreskin, and cultured in gelatin-based scaffolds. An in vivo study showed that the artificial dermis containing the fibroblasts enhanced the wound healing rate and re-epithelialization of a full-thickness skin defect rather than the acellular scaffold after one week.

Keywords:gelatin;β-glucan;hyaluronic acid;scaffold;skin

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[8] Meier S, Hay ED, Dev. Biol., 38, 249 (1974)

[9] Gey GO, Svoelis M, Foard M, Bang FB, Exp. Cell. Res., 84, 63 (1974)

[10] Postlethwaite AE, Seyer JM, Kang AH, Proc. Natl. Acad. Sci. USA, 75, 871 (1978)

[11] Law JK, Parsons JR, Silver FH, Weiss AB, J. Biomed. Mater. Res., 23, 961 (1989)

[12] Benedetti L, Cortivo R, Berti A, Pea F, Biomaterials, 14, 1154 (1993)

[13] Larsen NE, Pollak CT, Reiner K, Leshchiner E, Balazs E, J. Biomed. Mater. Res., 27, 1129 (1993)

[14] Karlsson G, Bergman R, J. Chromatogr. A, 986, 67 (2003)

[15] Sawai T, Uzuki M, Int. Congress Series, 1223, 273 (2001)

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[17] Bohn JA, Bemiller JN, Carbohyd. Polym., 28, 3 (1995)

[18] Yang K, Seo YK, Youn HH, Lee DH, Park SN, Park JK, Artif. Organs, 24, 7 (2000)

[19] Freshney RL, Culture of Animal Cells, A Manual of Basic Technique, 4th edition, Wiley-Liss Inc., pp. 345 (2000)

[20] Martine ML, Armstrong DW, Tissue Eng., 5, 153 (1999)

[21] Khang G, Lee SJ, Jeon JH, Lee JH, Lee HB, Polym.(Korea), 24(6), 869 (2000)

[22] Tamada Y, Ikada Y, J. Colloid Interface Sci., 155, 334 (1993)

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[30] Bernard C, Corinne L, Louis D, J. Invest. Dermatol., 92, 122 (1989)

[31] Singer AJ, Clark RAF, New. Engl. J. Med., 341, 738 (1999)

[32] Eaglstein WH, Iriondo M, Laszlo K, Dermatol. Surg., 21, 839 (1995)