Mixed-Template Imprinted Polymer의 분리 특성

김은철; 王德先; 노경호; Yinzhe Jin; Dexian Wang; Kyung Ho Row

Korean Chemical Engineering Research, Vol.42, No.4, 413-419, August, 2004

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Mixed-Template Imprinted Polymer의 분리 특성

Separation Characteristics of Mixed-Template Imprinted Polymer

김은철, 王德先, 노경호 ^†

인하대학교 생명화학공학부, 초정밀생물분리기술연구센터, 402-701 인천시 남구 용현동 253

Yinzhe Jin, Dexian Wang, and Kyung Ho Row^†

Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University, 253 Yonghyun-dong, Nam-gu, Incheon 402-751, Korea

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초록

분자 각인 고분자(molecular imprinted polymer, MIP)를 제조함에 있어서 주형분자로 각각 세 개의 단일성분인 pentoxifylline, caffeine, theophylline과 세 개의 혼합성분인 pentoxifylline-theophylline, pentoxifylline-caffeine, caffeine-theophylline을 사용하였다. 기능성 단량체로 MAA, 가교제로 EDGMA, 개시제로 AIBN를 사용하였다. 중합된 고분자(입자크기 25-35 μm)를 HPLC의 칼럽(3.9 × 150 mm)에 충진하고 acetonitrile을 이동상으로 하여 주형분자와 MIP의 인식원리를 연구하였고 혼합성분을 주형분자로 한 MIP의 인식 중에서 상호작용 효과에 대해 고찰하였다. 실험결과 두 혼합성분을 주형분자로 한 MIP에서의 체류인자와 분리도는 단일성분을 주형분자로 한 MIP보다 높았다.

Molecular imprinted polymers (MIP) by using single templates (pentoxifylline, caffeine and theophylline) and mixed-templates (pentoxifylline-theophylline pentoxifylline-caffeine, and caffeine-theophylline) were proposed in this paper. The MIP were prepared using MAA as the monomer, EGDMA as the crosslinker and AIBN as the initiator. The obtained polymer particles (particle size was about 25-35 μm) was packed into HPLC column (3.9 × 150 mm). The result of the experiment showed that the retention factors and resolutions of two mixed-template imprinting polymers were higher than that of single template imprinting polymers. This indicated increase the affinity of the MIP. The improved affinity on the mixed-template MIP was regarded as a result of cooperation effect of the binding sites.

Keywords:Molecular Imprinting;Mixed-Template Imprinted Polymer;Cooperative Effect

[References]

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Lang CD, Peng H, Bao XY, Nie LH, Yao SZ, Analyst, 124, 1781 (1999)
Yoshikawa M, Fujisawa T, Izumi J, Anal. Chim. Acta, 365, 59 (1998)
Zhou J, He X, Li Y, Anal. Chim. Acta, 394, 353 (1999)
Sajonz P, Kele M, Zhong G, Sellergren B, Guiochon G, J. Chromatogr. A, 810, 1 (1998)
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Zheng N, Li YZ, Chang WB, Wang ZM, Li TJ, Anal. Chim. Acta, 452, 277 (2002)
Cheong SH, Suh MG, Park JK, Karube I, HWAHAK KONGHAK, 36(1), 27 (1998)
Kempe M, Mosbach K, J. Chromatogr. A, 664, 276 (1994)
Xie J, Zhu L, Luo H, Zhou L, Li C, Xu X, J. Chromatogr. A, 934, 1 (2001)
Theodoridis G, Manesiotis P, J. Chromatogr. A, 948, 163 (2002)
Baggiani C, Trotta F, Giraudi G, Moraglio G, Vanni A, J. Chromatogr. A, 786, 23 (1997)
Kobayashi T, Murawaki Y, Reddy PS, Abe M, Fujii N, Anal. Chim. Acta, 435, 141 (2001)
Dauwe C, Sellergren B, J. Chromatogr. A, 753, 191 (1996)
Pap T, Horvath V, Tolokan A, Horvai G, Sellergre B, J. Chromatogr. A, 973, 1 (2002)

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[2] Matsui J, Nicholls IA, Takeuchi T, Anal. Chim. Acta, 365, 89 (1998)

[3] Bereczki A, Tolokan A, Horvai G, Horvath V, Lanza F, Hall AJ, Sellergren B, J. Chromatogr. A, 930, 31 (2001)

[4] Ansell RJ, Kriz D, Mosbach K, Curr. Opin. Biotechnol., 7, 89 (1996)

[5] Kriz O, Ramstrom O, Mosbach K, Anal. Chem., 69, 345A (1997)

[6] Lang CD, Peng H, Bao XY, Nie LH, Yao SZ, Analyst, 124, 1781 (1999)

[7] Yoshikawa M, Fujisawa T, Izumi J, Anal. Chim. Acta, 365, 59 (1998)

[8] Zhou J, He X, Li Y, Anal. Chim. Acta, 394, 353 (1999)

[9] Sajonz P, Kele M, Zhong G, Sellergren B, Guiochon G, J. Chromatogr. A, 810, 1 (1998)

[10] Owens PK, Karlsson L, Lutz ESM, Andersson LI, Trends Anal. Chem., 18, 146 (1999)

[11] Chen W, Liu F, Zhang X, Li KA, Tong S, Talanta, 55, 29 (2001)

[12] Zheng N, Li YZ, Chang WB, Wang ZM, Li TJ, Anal. Chim. Acta, 452, 277 (2002)

[13] Cheong SH, Suh MG, Park JK, Karube I, HWAHAK KONGHAK, 36(1), 27 (1998)

[14] Kempe M, Mosbach K, J. Chromatogr. A, 664, 276 (1994)

[15] Xie J, Zhu L, Luo H, Zhou L, Li C, Xu X, J. Chromatogr. A, 934, 1 (2001)

[16] Theodoridis G, Manesiotis P, J. Chromatogr. A, 948, 163 (2002)

[17] Baggiani C, Trotta F, Giraudi G, Moraglio G, Vanni A, J. Chromatogr. A, 786, 23 (1997)

[18] Kobayashi T, Murawaki Y, Reddy PS, Abe M, Fujii N, Anal. Chim. Acta, 435, 141 (2001)

[19] Dauwe C, Sellergren B, J. Chromatogr. A, 753, 191 (1996)

[20] Pap T, Horvath V, Tolokan A, Horvai G, Sellergre B, J. Chromatogr. A, 973, 1 (2002)