화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.30, No.3, 259-265, May, 2006
Export Citation
창상드레싱을 위한 Poly(vinyl alcohol)을 코팅한 소장점막하조직 시트의 제조와 특성 평가
Preparation and Characterization of Small Intestinal Submucosa Coated with Poly(vinyl alcohol) for Wound Dressing
이민숙, 양재찬1, 김순희1, 송인범1, 김문석2, 강길선†, 이해방2
  • 전북대학교 BK21 고분자 BIN융합연구팀
  • 1전북대학교 유기신물질공학과
  • 2한국화학연구원 나노생체재료연구팀
Min Suk Lee, Jea Chan Yang1, Soon Hee Kim1, In Bum Song1, Moon Suk Kim2, Gilson Khang†, and Hai Bang Lee2
  • BK21 Polymer BIN Fusion Res. Team, Chonbuk National University, 664-14 Dukjin, Jeonju 561-756, Korea
  • 1Department of Advanced Organic Materials Engineering, Chonbuk National University, 664-14 Dukjin, Jeonju 561-756, Korea
  • 2Medicinal Science Division, Korea Research Institute of Chemical Technology, P.O. Box 107, Yuseong, Daejon 305-600, Korea
E-mail:
초록
손상된 조직의 재생을 촉진시키는 여러가지 성장인자를 함유한 소장점막하조직(small intestinal subucosa, SIS)과 드레싱 교체시 손상된 피부로부터 박리가 용이한 수화젤 특성을 갖는 PVA를 이용하여 창상 치유를 촉진시키고 박리가 용이한 드레싱을 제조하였다. SIS 시트를 2, 4 및 10 wt%로 제조한 PVA 용액에 침지시킨 후 각기 다른 몰드에서 동결건조함으로써 PVA가 코팅된 SIS 시트를 제조하였다. 제조한 시트는 인장시험을 통해 기본 물성을 평가하였고, 분해도, 물 흡수성, 그리고 in vitro 실험을 통해 그 특성을 조사하였다. PVA를 코팅한 SIS(PVA-SIS) 시트의 인장강도와 신장률은 PVA의 함량이 증가함에 따라 각각 감소, 증가하였으며, 분해도는 본래의 SIS에 비해 감소하였고, SIS에 2 및 4 wt%의 PVA가 코팅되었을 때 물 흡수성이 증가하였다. 또한 전 PVA-SIS 시트에서 세포부착도가 본래의 SIS 시트에 비해 낮음을 확인하였다. 이로써 PVA를 코팅한 SIS 시트가 창상 치료를 위한 드레싱제와 생분해성 이식 제제로서 사용가능하리라 판단되었다.
Small intestinal submucosa(SIS) consists of some growth factors which can stimulate cell activity, and PVA has been widely utilized in the area of wound dressing as hydrogel which is easy to be removed from wounds. In this study, native SIS sheets were coated with PVA by immersing them into 2, 4, and 10 wt% of PVA solution and then lyophilized on two type of molds to endow the prepared wound dressing with easy removal property from wounds. The mechanical properties were examined through tensile test. Moreover, enzymatic degradation, water uptake, and in vitro test were carried out to characterize the prepared SIS sheets. The tensile strength of the SIS sheets coated with PVA (PVA-SIS) were decreased, whereas the elongation were increased. Degradation ratio of the PVA-SIS sheets was decreased compare to native SIS. Water uptake ability was improved at 2 and 4 wt% of PVA. The degree of fibroblast attachment was lower than the native SIS sheets. In conclusion, this study suggests that the PVA coated SIS sheets have a potential for the applications of wound dressing and biodegradable injectable materials.
Keywords:small intestinal submucosa;poly(vinyl alcohol);wound dressing
  1. Carenza M, Radiat. Phys. Chem., 39, 485 (1992)
  2. Cascone MG, Barbani N, Cristallini C, Giusti P, Ciardelli G, Lazzeri L, J. Biomater. Sci.-Polym. Ed., 12, 267 (2001) 
  3. Crescenzi V, Paradossi G, Desideri P, Dentini M, Cabalieri F, Amici E, Lisi R, Polymer Gels Network, 5, 225 (1997) 
  4. Harxh DC, Gehrake SH, J. Control. Release, 17, 175 (1991) 
  5. Peppas NA, Scott JE, J. Control. Release, 18, 95 (1992) 
  6. Higa OZ, Rogero SO, Machado LDB, Mathor MB, Lugao AB, Radiat. Phys. Chem., 55, 705 (1999) 
  7. Zhai M, Yoshii F, Kume T, Hashim K, Carbohydr. Polym., 50, 295 (2002) 
  8. Zhao L, Mitomo H, Zhai M, Yoshii F, Nagasawa N, Kume T, Carbohydr. Polym., 53, 439 (2003) 
  9. Rosen M, Ponsky J, Petras R, Fanning A, Brody F, Duperier F, Surgery, 132, 480 (2002) 
  10. Hodde JP, Tissue Eng., 8, 295 (2002) 
  11. Hodde JP, Badylak SF, Brightman AO, Voytik-Harbin SL, Tissue Eng., 2, 209 (1996)
  12. McPherson TB, Badylak SF, Tissue Eng., 4, 75 (1998)
  13. Shon MJ, Lee SY, Kim TH, You CK, Kim S, Choung PH, Son Y, Park EK, Kim SY, Tissue Eng. Regen. Med., 2, 356 (2005)
  14. Lee SY, Shon MJ, Kim TH, Kim S, Son Y, Kim SY, Park EK, Tissue Eng. Regen. Med., 2, 362 (2005)
  15. Hodde JP, Hiles MC, Wounds, 13, 195 (2001)
  16. Hodde JP, Ernst DMJ, Hiles MC, J. Wound Care, 14, 23 (2005)
  17. McDevitt CA, Wildey GM, Cutrone RM, Biomed J, Mater. Res., 67A, 637 (2003) 
  18. Khang G, Kim MS, Cho SH, Lee I, Rhee JM, Lee HB, Tissue Eng. Regen. Med., 1, 9 (2004)
  19. Kim MS, Hong KD, Shin HW, Kim SH, Kim SH, Lee MS, Jang WY, Khang S, Lee HB, Int. J. Biol. Macromol., 36, 54 (2005) 
  20. Park WH, Lee JK, Polym.(Korea), 21(1), 46 (1997)
  21. Martien FL, Encyclopedia of Polym. Sci. Eng., New York, Wiley, vol.17, p.167 (1986)
  22. Seal BL, Otero TC, Panitch A, Mater. Sci. Eng., 34, 147 (2001) 
  23. Huglin MB, Zakaria MB, J. Appl. Polym. Sci., 31, 457 (1986) 
  24. Yishii F, Zhashan Y, Isobe K, Shinozaki K, Makuchi K, J. Membr. Sci., 109, 257 (1999) 
  25. Cho KJ, Song DK, Oh SH, Koh YJ, Lee SH, Lee MC, Lee JH, Tissue Eng. Regen. Med., 2, 115 (2005)
  26. Khang G, Rhee JM, Shin P, Kim IY, Lee B, Lee SJ, Lee YM, Lee HB, Lee I, Macromol. Res., 10(3), 158 (2002)
  27. Clark KM, Lantz GC, Salibury SK, Badylak SF, Hiles MC, Voytik SL, J. Surg. Res., 60, 107 (1996) 
  28. Shettigar UT, Jogannathan R, Natarajan R, Artif. Organs, 6, 256 (1982)
  29. Han SK, Choi SJ, "The Demand of Dressing", in Advance in Wound Care, H. J. Yoo, J. H. Kim, M. H. Park, K. K. Lee, J. H. Lee, Y. S. Yoo, S. K. Han, and G. Yushimize, Editors, Korea Medical Book Publisher Co., Seoul, pp 170 (2003)
  30. Rosen M, Ponsky J, Petras R. Fanning A, Brody F, Duperier F, Surgery, 132, 480 (2002) 
  31. Schmedlen RH, Kristyn KS, Jennifer JL, Biomaterials, 23, 4325 (2002)