BGsome is liquid crystalline vesicle prepared by hydration of butylene glycol(BG) - dissolved lecithin with a solution containing hydrophilic component. Investigation of factors affecting the entrapment efficiency and particle size of ethosones was carried out. The variations of properties of BGsome with constituent composition and preparation method were examined using a calcein as hydrophilic marker. It was observed that the amount of BG and calcein solution, phosphatidyl choline content in lectihin, and preparation temperature had a considerable effect on the properties of ethosome.