Inhibition of melanogenesis and melanin transportation by Gynostemma pentaphyllum

Hye In Lee; Byoung Sam Yoo; Mi Ae Yoo; Sang Yo Byun

Korean Journal of Chemical Engineering, Vol.24, No.4, 655-659, July, 2007

DOI10.1007/s11814-007-0020-z Export Citation

Inhibition of melanogenesis and melanin transportation by Gynostemma pentaphyllum

Hye In Lee, Byoung Sam Yoo¹, Mi Ae Yoo², and Sang Yo Byun^†

Department of Molecular Science & Technology, Ajou University, Suwon, Kyunggi 443-749, Korea
¹Cosmetic R&D Center, COSMAX Inc., Hwa Sung, Kyunggi 445-746, Korea
²Department of Applied Biotechnology, Ajou University, Suwon, Kyunggi 443-749, Korea

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The extract of Gynostemma pentaphyllum was tested to control the melanogenesis in B16 melanoma. Cytotoxic effect by the extract was observed when the dose concentration was higher than 2 mg/L. Most of the inhibitory effect was obtained by the reduced accumulation of extra-cellular melanin. When the extract was dosed as 2 mg/L, the extra-cellular melanin produced was only 24% of the control. Proteome analysis with 2-D PAGE showed that various proteins involved in melanogenesis were down-regulated by Gynostemma pentaphyllum. In addition to other proteins related to the intra-cellular melanogenesis, Rab-27b and Rab-38 could explain the remarkable decrease in extra-cellular melanin accumulation by reduced melanin transfer to keratinocyte.

Keywords:Gynostemma pentaphyllum;Melanogenesis;Inhibition;2-D PAGE

[References]

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Jimbow M, Kanoh H, Jimbow K, J. Invest. Dermatol., 79, 97 (1982)
Shola SS, Barbara EK, Veterinary Dermatology, 14, 57 (2003)
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Sober AJ, Fitzpatrick TB, Prog. Chem. Org. Natural Products, 31, 521 (1974)
Karine B, Elizabeth L, James LF, Yiqun GS, Gretchen A, Kirsi P, Allen DE, Kenji F, David AN, Natalie GA, Katheryn AR, Cancer Res., 63, 6716 (2003)
Valentina RN, Huang TWH, Tran VH, Li GQ, Duke C, Roufogalis BD, Phys. Rev., 4, 197 (2005)
Liu HL, Kao TH, Chen BH, Chromatographia, 60, 411 (2004)
Lee GW, Byun SY, Biotechnol. Bioprocess Eng., 8, 299 (2003)
Sieuwerts AM, Klijn JGM, Peters HA, Foekens JA, Eur. J. Clin. Chem. Clin. Biochem., 33, 813 (1995)
Hosoi J, Abe E, Suda T, Kuroki T, Cancer Res., 45, 1474 (1985)
Bradford MM, Anal. Biochem., 72, 248 (1976)
Gorg A, Obermaier C, Boguth G, Harder A, Scheibe B, Wildgruber R, Weiss W, Electrophoresis, 21, 1037 (2000)
Lee GW, Yoon HC, Byun SY, Enzyme Microb. Technol., 35(6-7), 632 (2004)
Fuller BB, Spaulding DT, Smith DR, Exp. Cell Res., 262, 197 (2001)
Puri N, Gardner JM, Brilliant MH, J. Invest. Dermatol., 115, 607 (2000)
Kameyama K, Takemura T, Hamada Y, Sakai C, Kondoh S, Nishiyama S, Urabe K, Hearing VJ, J. Invest. Dermatol., 100, 126 (1993)
Graphodatskaya D, Joerg H, Stranzinger G, J. Recept. Signal Transduct Res., 22, 421 (2002)
Schepper S, Boucneau JM, Westbroek W, Mommaas M, Onderwater J, Messiaen L, Naeyaert JM, Lambert JL, J. Invest. Dermatol., 126, 635 (2006)

[Referenced By]

[1] Venkatesha B, Feng Y, Tsuneto K, Youichiro H, Ken-Ichi Y, Julio V, Jacqueline M, Wilfred DV, Hidenori W, Jeffrey J, Vincent JH, Donald FH, Ettore A, J. Proteome Research, 2, 69 (2003)

[2] Jimbow M, Kanoh H, Jimbow K, J. Invest. Dermatol., 79, 97 (1982)

[3] Shola SS, Barbara EK, Veterinary Dermatology, 14, 57 (2003)

[4] Prota G, J. Invest. Dermatol., 75, 122 (1980)

[5] Sober AJ, Fitzpatrick TB, Prog. Chem. Org. Natural Products, 31, 521 (1974)

[6] Karine B, Elizabeth L, James LF, Yiqun GS, Gretchen A, Kirsi P, Allen DE, Kenji F, David AN, Natalie GA, Katheryn AR, Cancer Res., 63, 6716 (2003)

[7] Valentina RN, Huang TWH, Tran VH, Li GQ, Duke C, Roufogalis BD, Phys. Rev., 4, 197 (2005)

[8] Liu HL, Kao TH, Chen BH, Chromatographia, 60, 411 (2004)

[9] Lee GW, Byun SY, Biotechnol. Bioprocess Eng., 8, 299 (2003)

[10] Sieuwerts AM, Klijn JGM, Peters HA, Foekens JA, Eur. J. Clin. Chem. Clin. Biochem., 33, 813 (1995)

[11] Hosoi J, Abe E, Suda T, Kuroki T, Cancer Res., 45, 1474 (1985)

[12] Bradford MM, Anal. Biochem., 72, 248 (1976)

[13] Gorg A, Obermaier C, Boguth G, Harder A, Scheibe B, Wildgruber R, Weiss W, Electrophoresis, 21, 1037 (2000)

[14] Lee GW, Yoon HC, Byun SY, Enzyme Microb. Technol., 35(6-7), 632 (2004)

[15] Fuller BB, Spaulding DT, Smith DR, Exp. Cell Res., 262, 197 (2001)

[16] Puri N, Gardner JM, Brilliant MH, J. Invest. Dermatol., 115, 607 (2000)

[17] Kameyama K, Takemura T, Hamada Y, Sakai C, Kondoh S, Nishiyama S, Urabe K, Hearing VJ, J. Invest. Dermatol., 100, 126 (1993)

[18] Graphodatskaya D, Joerg H, Stranzinger G, J. Recept. Signal Transduct Res., 22, 421 (2002)

[19] Schepper S, Boucneau JM, Westbroek W, Mommaas M, Onderwater J, Messiaen L, Naeyaert JM, Lambert JL, J. Invest. Dermatol., 126, 635 (2006)