The formation of amyloid fibril-like hen egg-white lysozyme species induced by temperature and urea concentration-dependent denaturation

Steven S.-S. Wang; Ying-Tz Hung; Pu Wang; Josephine W. Wu

Korean Journal of Chemical Engineering, Vol.24, No.5, 787-795, September, 2007

DOI10.1007/s11814-007-0042-6 Export Citation

The formation of amyloid fibril-like hen egg-white lysozyme species induced by temperature and urea concentration-dependent denaturation

Steven S.-S. Wang^†, Ying-Tz Hung, Pu Wang, and Josephine W. Wu

Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan

E-mail:

Recent evidence has suggested that the formation of partially unfolded denatured intermediate serves as a crucial prerequisite for protein fibril formation/aggregation. Despite extensive exploration on amyloid fibril formation, the detailed molecular mechanism has remained largely unknown. Here, we examined the effects of urea on the denaturation of hen egg-white lysozymes. Our results demonstrated that, in the solutions (pH 7.4) with 8M urea at all temperatures and with 4M urea at 45 or 55 ℃, the amyloid fibril-like species were first produced and then vanished while such species were not observed under other conditions. In addition, SDS-PAGE results further indicated that larger aggregated species with less ordered structures were formed at the later stage of the urea-induced unfolding process owing to an unidentified conformational switch. We believe that the results in this work will aid in deciphering the molecular mechanism(s) of protein denaturation.

Keywords:Lysozyme;Amyloid;Fibril;Aggregation;Urea;Partial Unfolding;Denaturation

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[Referenced By]

[1] Fink AL, Phys. Rev., 79, 425 (1999)

[2] Houry WA, Curr. Protein. Pept. Sci., 2, 227 (2001)

[3] Markossian KA, Kurganov BI, Biochemistry, 69, 971 (2004)

[4] Young JC, Agashe VR, Siegers K, Hartl FU, Nat. Rev. Mol. Cell Biol., 5, 781 (2004)

[5] Dobson CM, Semin. Cell Dev. Biol., 15, 3 (2004)

[6] Murphy RM, Annu. Rev. Biomed. Eng., 4, 155 (2002)

[7] Ross CA, Poirier MA, Nat. Med., 10, S10 (2004)

[8] Uversky VN, Fink AL, Biochem. Biophys. Acta, 1698, 131 (2004)

[9] Lai Z, Colon W, Kelly JW, Biochemistry, 35, 6470 (1996)

[10] Ferrao-Gonzales AD, Souto SO, Silva JL, Foguel D, Proc. Natl. Acad. Sci. USA, 97, 6445 (2000)

[11] Sluzky V, Klibanov AM, Langer R, Biotechnol. Bioeng., 40, 895 (1992)

[12] Beaven GH, Gratzer WB, Davies HG, Eur. J. Biochem., 11, 37 (1969)

[13] Chiti F, Webster P, Taddei N, Clark A, Stefani M, Ramponi G, Dobson CM, Proc. Natl. Acad. Sci. USA, 96, 3590 (1999)

[14] Fandrich M, Forge V, Buder K, Kittler M, Dobson CM, Diekmann S, Proc. Natl. Acad. Sci. USA, 100, 15463 (2003)

[15] Kallberg Y, Gustafsson M, Persson B, Thyberg J, Johansson J, J. Biol. Chem., 276, 12945 (2001)

[16] Ji SR, Wu Y, Sui SF, Gen. Physiol. Biophys., 21, 415 (2002)

[17] Lansbury PT, Proc. Natl. Acad. Sci. USA, 96, 3342 (1999)

[18] Soto C, J. Mol. Med., 77, 412 (1999)

[19] Stefani M, Dobson CM, J. Mol. Med., 81, 678 (2003)

[20] Wang SSS, Good TA, J. Chin. Inst. Chem. Eng., 36, 533 (2005)

[21] De Felice FG, Vieira MN, Saraiva LM, Figueroa-Villar JD, Garcia-Abreu J, Liu R, Chang L, Klein WL, Ferreira ST, Faseb J., 18, 1366 (2004)

[22] Schuster D, Rajendran A, Hui SW, Nicotera T, Srikrishnan T, Kruzel ML, Neuropeptides (2005)

[23] Wang SS, Good TA, Rymer DL, Int. J. Biochem. Cell Biol., 37, 1656 (2005)

[24] Rochet JC, Conway KA, Lansbury PT, Biochemistry, 39, 10619 (2000)

[25] Zerovnik E, Eur. J. Biochem., 269, 3362 (2002)

[26] Dobson CM, Philos. Trans. R. Soc. Lond. B. Biol. Sci., 356, 133 (2001)

[27] Ohnishi S, Takano K, Cell Mol. Life Sci., 61, 511 (2004)

[28] Vaney MC, Maignan S, Ries-Kautt M, Ducriux A, Acta. Crystallogr. D. Biol. Crystallogr., 52, 505 (1996)

[29] Pepys MB, Hawkins PN, Booth DR, Vigushin DM, Tennent GA, Soutar AK, Totty N, Nguyen O, Blake CC, Terry CJ, Nature, 362, 553 (1993)

[30] Fischer BE, Biotechnol. Adv., 12, 89 (1994)

[31] Gao YG, Guan YX, Yao SJ, Cho MG, Korean J. Chem. Eng., 19(5), 871 (2002)

[32] Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC, Anal. Biochem., 150, 76 (1985)

[33] Klunk WE, Pettegrew JW, Abraham DJ, J. Histochem. Cytochem., 37, 1273 (1989)

[34] Liu CP, Li ZY, Huang GC, Perrett S, Zhou JM, Biochem., 87, 1023 (2005)

[35] Smoot AL, Panda M, Brazil BT, Buckle AM, Fersht AR, Horowitz PM, Biochemistry, 40, 4484 (2001)

[36] Sirangelo I, Bismuto E, Tavassi S, Irace G, Biochem. Biophys. Acta., 1385, 69 (1998)

[37] LeVine H, Protein. Sci., 2, 404 (1993)

[38] Vernaglia BA, Huang J, Clark ED, Biomacromolecules, 5, 1362 (2004)

[39] Kelly JW, Curr. Opin. Struct. Biol., 8, 101 (1998)

[40] Sipe JD, Crit. Rev. Clin. Lab. Sci., 31, 325 (1994)

[41] Sluzky V, Tamada JA, Klibanov AM, Langer R, Proc. Natl. Acad. Sci. USA, 88, 9377 (1991)

[42] Quintas A, Vaz DC, Cardoso I, Saraiva MJ, Brito RM, J. Biol. Chem., 276, 27207 (2001)

[43] McDuff FO, Doucet A, Beauregard M, Biochem. Cell Biol., 82, 305 (2004)

[44] Nielsen L, Khurana R, Coats A, Frokjaer S, Brange J, Vyas S, Uversky VN, Fink AL, Biochemistry, 40, 6036 (2001)

[45] Uversky VN, Li J, Fink AL, J. Biol. Chem., 276, 10737 (2001)

[46] Souillac PO, Uversky VN, Millett IS, Khurana R, Doniach S, Fink AL, J. Biol. Chem., 277, 12657 (2002)

[47] Khurana R, Gillespie JR, Talapatra A, Minert LJ, Ionescu-Zanetti C, Millett I, Fink AL, Biochemistry, 40, 3525 (2001)

[48] Goers J, Permyakov SE, Permyakov EA, Uversky VN, Fink AL, Biochemistry, 41, 12546 (2002)

[49] Speed MA, Morshead T, Wang DI, King J, Protein. Sci., 6, 99 (1997)