Disulfide-linked manganese mesoporphyrin derivatives separated by spacer methylene groups (Figure 1) were synthesized and then the porphyrin derivatives were assembled on a gold electrode. Cyclic voltammetry (CV) of the porphyrin monolayer on a gold electrode showed that one set of waves was clearly visible, corresponding to the consecutive monoelectronic reduction of the manganese porphyrin unit, depending upon the spacer methylene groups. Interestingly, a large positive-shift of the redox potential for the mesoporphyrin was observed due to fluorination of the porphyrin ring.