Poly(γ-benzyl L-glutamate)/Poly(ethylene oxide)블록 공중합체의 합성과 항혈전성: Poly(ethylene oxide)분자량의 영향

조종수; 김희용; 송수창; 박중양; 권중근

Polymer(Korea), Vol.15, No.2, 118-123, April, 1991

Synthesis and Blood Compatibility of Poly(γ-benzyl L-glutamate)/Poly(ethylene oxide) Triblock Copolymers : Effect of Molecular Weight of Poly(ethylene oxide)

조종수, 김희용, 송수창, 박중양, 권중근

초록

poly(γ-benzyl L-glutamate)를 A성분으로, poly(ethylene oxide)를 B성분으로 구성된 ABA형의 블록공중합체가 PEO의 함량은 일정하게 하고 분자량을 다르게 하여 합성되었다. 용액상에서의 CD측정과 고체상에서의 IR측정으로 이 블록공중합체가 PBLG homepolymer에서와 같이 전형적인 α-helix 구조를 갖고 있음을 알았다. In vitro에서의 항혈전실험으로부터, 이 블록공중합 표면에서의 혈소판의 흡착정도는 블록공중합체의 PEO 분자량에 의존함을 알았고, 특히 PEO 분자량이 4000인 블록공중합체에서 혈소판의 점착이 가장 적음을 보였다.

ABA type triblock copolymers consisting of poly(γ-benzyl L-glutamate) (PBLG) as the A component and poly(ethylene oxide) (PEO) as the B component were synthesized. Various molecular weight of PEO was utilized to study the effect of PEO molecular weight on blood compatibility. From circular dichroism measurements in solution as well as infrared spectra measurements in the solid state, it was found that the block copolymers exist in the α-helical conformation as in PBLG homopolymer. From the in vitro antithrombogenic test, it was found that antithrombogenicity of the block copolymer is dependant on the molecular weight of PEO in the block copolymer. The best blood compatibility was obtained at PEO molecular weight 4000.

[References]

Sakurai Y, Akaike T, Kataoka K, Okano T, "Interfacial Phenomena in Biomaterial Chemistry," A. Nakajima and E. Goldberg, Eds., Academic Press, New York, 335 (1980)
Anderson JM, Marchant KK, CRC Critical Rev. Biocompatibility, 1, 111 (1985)
Okano T, Uruno M, Sugiyama N, Shinohara I, Kataoka K, Sakurai Y, J. Biomed. Mater. Res., 20, 1035 (1986)
Okano T, Nishiyama S, Shinohara I, Akaike T, Kataoka K, Tsuruta T, J. Biomed. Mater. Res., 15, 393 (1981)
Sawyer PN, Burrowes C, Ogoniak J, Smith AO, Wesolowski SA, Trans. Am. Soc. Artif. Int. Organs., 10, 316 (1964)
Cho CS, Kim SW, Makromol. Chem., 189, 1505 (1988)
Cho CS, Song SC, Suh SP, Kim KY, Jang SW, Sung YK, Polym.(Korea), 13(8), 657 (1989)
Cho CS, Kim SW, Komoto T, Makromol. Chem., 191, 981 (1990)
Cho CS, Kim HY, Song SC, Yang DW, Kim JO, Kim SS, Polym.(Korea), to be accepted (1990)
Nagaoka S, Mori Y, Takiuchi H, Yokota K, Tanzawa H, Nishu S, "Polymers as Biomaterials," S.W. Shalaby, A.S. Hoffman, B.E. Ratner, and T.A. Horbett, ed., p. 361-374, Plenum Press (1984)
Nagaoka S, Kurumatani H, Mori Y, Tanzawa H, J. Bioactive Compatible Polym., 4, 323 (1989)
Park KD, Okano T, Nojiri C, Kim SW, J. Biomed. Mater. Res., 22, 977 (1988)
Takahara A, Jo NJ, Kajiyama T, J. Biomater. Sci.-Polym. Ed., 1, 17 (1989)
Lee JH, Andrade JD, "Surface Properties of Aqueous PEO/PPO Block Copolymer Surfactants," in Polymer Surface Dynamics, J.D. Andrade (Ed.), Plenum Press, New York, pp. 119-136 (1988)
Fuller WD, Verlander MS, Goodman M, Biopolymers, 15, 869 (1976)
Alter JE, Taylor GT, Scheraga HA, Macromolecules, 5, 739 (1972)
Hashimoto Y, Aoyama A, Imanishi Y, Higashimura T, Biopolymers, 15, 2407 (1976)
Doty P, Bradbury JH, Holtzer AM, J. Am. Chem. Soc., 78, 947 (1956)
Holtzwarth G, Doty P, J. Am. Chem. Soc., 87, 218 (1965)

[1] Sakurai Y, Akaike T, Kataoka K, Okano T, "Interfacial Phenomena in Biomaterial Chemistry," A. Nakajima and E. Goldberg, Eds., Academic Press, New York, 335 (1980)

[2] Anderson JM, Marchant KK, CRC Critical Rev. Biocompatibility, 1, 111 (1985)

[3] Okano T, Uruno M, Sugiyama N, Shinohara I, Kataoka K, Sakurai Y, J. Biomed. Mater. Res., 20, 1035 (1986)

[4] Okano T, Nishiyama S, Shinohara I, Akaike T, Kataoka K, Tsuruta T, J. Biomed. Mater. Res., 15, 393 (1981)

[5] Sawyer PN, Burrowes C, Ogoniak J, Smith AO, Wesolowski SA, Trans. Am. Soc. Artif. Int. Organs., 10, 316 (1964)

[6] Cho CS, Kim SW, Makromol. Chem., 189, 1505 (1988)

[7] Cho CS, Song SC, Suh SP, Kim KY, Jang SW, Sung YK, Polym.(Korea), 13(8), 657 (1989)

[8] Cho CS, Kim SW, Komoto T, Makromol. Chem., 191, 981 (1990)

[9] Cho CS, Kim HY, Song SC, Yang DW, Kim JO, Kim SS, Polym.(Korea), to be accepted (1990)

[10] Nagaoka S, Mori Y, Takiuchi H, Yokota K, Tanzawa H, Nishu S, "Polymers as Biomaterials," S.W. Shalaby, A.S. Hoffman, B.E. Ratner, and T.A. Horbett, ed., p. 361-374, Plenum Press (1984)

[11] Nagaoka S, Kurumatani H, Mori Y, Tanzawa H, J. Bioactive Compatible Polym., 4, 323 (1989)

[12] Park KD, Okano T, Nojiri C, Kim SW, J. Biomed. Mater. Res., 22, 977 (1988)

[13] Takahara A, Jo NJ, Kajiyama T, J. Biomater. Sci.-Polym. Ed., 1, 17 (1989)

[14] Lee JH, Andrade JD, "Surface Properties of Aqueous PEO/PPO Block Copolymer Surfactants," in Polymer Surface Dynamics, J.D. Andrade (Ed.), Plenum Press, New York, pp. 119-136 (1988)

[15] Fuller WD, Verlander MS, Goodman M, Biopolymers, 15, 869 (1976)

[16] Alter JE, Taylor GT, Scheraga HA, Macromolecules, 5, 739 (1972)

[17] Hashimoto Y, Aoyama A, Imanishi Y, Higashimura T, Biopolymers, 15, 2407 (1976)

[18] Doty P, Bradbury JH, Holtzer AM, J. Am. Chem. Soc., 78, 947 (1956)

[19] Holtzwarth G, Doty P, J. Am. Chem. Soc., 87, 218 (1965)