Microchip-based cell aggregometer using stirring-disaggregation mechanism

Sehyun Shin; Yijie Yang; Jang-Soo Suh

Korea-Australia Rheology Journal, Vol.19, No.3, 109-115, November, 2007

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Microchip-based cell aggregometer using stirring-disaggregation mechanism

Sehyun Shin^†, Yijie Yang¹, and Jang-Soo Suh²

Department of Mechanical Engineering, Korea University, Seoul, Korea
¹School of Mechanical Engineering, Kyungpook National University, Daegu, Korea
²Department of Laboratory Medicine, Kyungpook National University Hospital, Daegu, Korea

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A new microchip-based aggregometer that uses a stirring-aided disaggregation mechanism in a microchip was developed to measure red blood cell (RBC) aggregation in blood and RBC suspensions. Conventional methods of RBC disaggregation, such as the rotational Couette system, were replaced with a newly designed stirring-induced disaggregation mechanism. Using a stirrer in a microchip, the aggregated RBCs stored in a microchip can be easily disaggregated. With an abrupt halt of the stirring, the backscattered light intensity can be measured in a microchip with respect to time. The time recording of the backscattered light intensity (syllectogram) shows an exponential decreasing curve representing the RBC aggregation. By analyzing the syllectogram, aggregation indices such as AI and M were determined. The results showed excellent agreement with LORCA. The essential feature of this design is the incorporation of a disposable microchip and the stirring-induced disaggregation mechanism.

Keywords:aggregation;RBC;microchip;aggregometer;stirring

[References]

Baskurt OK, Temiz A, Meiselman HJ, J. Lab. Clin. Med., 130, 183 (1997)
Bauersachs RM, Shaw S, Zeidler A, Meiselman HJ, Clin. Hemorheol., 7, 432 (1987)
Boynard M, Lelievre JC, Guillet R, Biorheology, 24, 451 (1987)
Chien S, Jan KM, Microvasc. Res., 5, 155 (1973)
Dormandy L, Ernst E, Matrai A, Flute PT, Am. Heart J., 104, 1364 (1982)
Hardeman MR, Goedhart PT, Dobbe JGG, Lettinga KP, Clin. Hemorheol., 14, 605 (1994)
Hein HJ, Bauersachs RM, Feinstein EI, Meiselman HJ, Clin. Hemorheol., 7, 425 (1987)
Meiselman HJ, Baskurt OK, Clin. Hemorheol. Microcirc., 35, 37 (2006)
Neu B, Meiselman HJ, Biophys. J., 83, 2482 (2002)
Pribush A, Meiselman HJ, Meyerstein D, Meyerstein N, Biorheology, 36, 411 (1999)
Rampling MW, Red cell aggregation and yield stress, in Clinical Blood Rheology Vol. edited by G.D. Lowe, CRC Press, Boca Raton, Florida, USA, 46-64 (1988)
Rampling MW, Meiselman HJ, Neu B, Baskurt OK, Biorheology, 41, 91 (2004)
Shvartsman LD, Fine I, SPIE proc., 4263, 131 (2001)
Shin S, Park MS, Jang JH, Ku YH, Suh JS, Korea-Aust. Rheol. J., 16(3), 129 (2004)
Shin S, Jang JH, Park MS, Ku YH, Suh JS, Korea-Aust. Rheol. J., 17(1), 9 (2005)
Stoltz JF, Singh M, Riha P, Hemorheology in Practice., IOS Press, Amsterdam
Zhao H, Wang X, Stolz JF, Clin. Hemorheol. Microcirc., 21, 297 (1999)

[Referenced By]

[1] Baskurt OK, Temiz A, Meiselman HJ, J. Lab. Clin. Med., 130, 183 (1997)

[2] Bauersachs RM, Shaw S, Zeidler A, Meiselman HJ, Clin. Hemorheol., 7, 432 (1987)

[3] Boynard M, Lelievre JC, Guillet R, Biorheology, 24, 451 (1987)

[4] Chien S, Jan KM, Microvasc. Res., 5, 155 (1973)

[5] Dormandy L, Ernst E, Matrai A, Flute PT, Am. Heart J., 104, 1364 (1982)

[6] Hardeman MR, Goedhart PT, Dobbe JGG, Lettinga KP, Clin. Hemorheol., 14, 605 (1994)

[7] Hein HJ, Bauersachs RM, Feinstein EI, Meiselman HJ, Clin. Hemorheol., 7, 425 (1987)

[8] Meiselman HJ, Baskurt OK, Clin. Hemorheol. Microcirc., 35, 37 (2006)

[9] Neu B, Meiselman HJ, Biophys. J., 83, 2482 (2002)

[10] Pribush A, Meiselman HJ, Meyerstein D, Meyerstein N, Biorheology, 36, 411 (1999)

[11] Rampling MW, Red cell aggregation and yield stress, in Clinical Blood Rheology Vol. edited by G.D. Lowe, CRC Press, Boca Raton, Florida, USA, 46-64 (1988)

[12] Rampling MW, Meiselman HJ, Neu B, Baskurt OK, Biorheology, 41, 91 (2004)

[13] Shvartsman LD, Fine I, SPIE proc., 4263, 131 (2001)

[14] Shin S, Park MS, Jang JH, Ku YH, Suh JS, Korea-Aust. Rheol. J., 16(3), 129 (2004)

[15] Shin S, Jang JH, Park MS, Ku YH, Suh JS, Korea-Aust. Rheol. J., 17(1), 9 (2005)

[16] Stoltz JF, Singh M, Riha P, Hemorheology in Practice., IOS Press, Amsterdam

[17] Zhao H, Wang X, Stolz JF, Clin. Hemorheol. Microcirc., 21, 297 (1999)