Applications of silver nanoplates as colorimetric indicators of pH-induced conformational changes in cytochrome c

Junsu Park; Do-Young Yoon; Younghun Kim

Korean Journal of Chemical Engineering, Vol.26, No.1, 258-260, January, 2009

DOI10.1007/s11814-009-0043-8 Export Citation

Applications of silver nanoplates as colorimetric indicators of pH-induced conformational changes in cytochrome c

Junsu Park, Do-Young Yoon , and Younghun Kim^†

Department of Chemical Engineering, Kwangwoon University, Wolgye-dong, Nowon-gu, Seoul 139-701, Korea

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Silver nanoplates (AgNPs) were used as a colorimetric indicator of pH-induced conformational changes in the protein, cytochorme c (cyt c). Cyt c was covalently bound to the surface of AgNPs, and UV-vis spectroscopy was used to identify changes in the optical properties of the resulting cyt c-AgNP conjugates in colloidal solution over pH, with values ranging from 3.1 to 10.6. Transmission electron microscopy (TEM) results indicated that the morphological changes in cyt c-AgNP conjugates were dependent on pH. Moreover, pH-induced conformational changes in cyt c could be detected by visual inspection of the color changes in cyt c-AgNPs, which occurred as a result of the coupling effect of localized surface plasmon resonance (LSPR) by aggregated AgNPs.

Keywords:Silver Nanoparticle;Colorimetric;Cytochrome;pH

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[Related Articles]

[1] Mirkin CA, Letsinger RL, Mucic RC, Storhoff JJ, Nature, 382(6592), 607 (1996)

[2] Choi S, Kim KS, Yeon SH, Cha JH, Lee H, Kim CJ, Yoo ID, Korean J. Chem. Eng., 24(5), 856 (2007)

[3] Kelly KL, Coronado E, Zhao LL, Schatz GC, J. Phys. Chem., 107, 668 (2003)

[4] Haes AJ, Zou SL, Schatz GC, Van Duyne RP, J. Phys. Chem. B, 108(22), 6961 (2004)

[5] Elghanian R, Storhoff JJ, Mucic RC, Letsinger RL, Mirkin CA, Science, 277(5329), 1078 (1997)

[6] Chah S, Hammond MR, Zare RN, Chem Biol., 12, 323 (2005)

[7] Worrall JWE, Verma A, Yan H, Rotello VM, Chem. Comm., 22, 2338 (2006)

[8] Salafsky JS, J. Chem. Phys., 125, 074701 (2006)

[9] Jiang X, Qu X, Zhang L, Zhang Z, Jiang J, Wang E, Dong S, Biophys. Chem., 110, 203 (2004)

[10] Chah S, Kumar CV, Hammond MR, Zare RN, Anal. Chem., 76, 2112 (2004)

[11] Jiang X, Zeng Q, Yu A, Nanotechnology, 17, 4929 (2006)

[12] Ferrer JC, Guillemette JG, Bogumil R, Inglis SC, Smith M, Mauk AG, J. Am. Chem. Soc., 115, 7507 (1993)

[13] Santucci R, Bongiovanni C, Mei G, Ferri T, Polizio F, Desideri A, Biochemistry, 39, 1632 (2000)

[14] Garrett RH, Grisham CM, Biochemistry, Harcourt, Orlando, FL (1999)

[15] Li GT, Wang TY, Bhosale S, Zhang Y, Fuhrhop JH, Colloid Polym. Sci., 281, 1099 (2003)

[16] Han X, Li Y, Wu S, Deng Z, Small, 4, 326 (2008)

[17] Chen Y, Wang C, Ma Z, Su Z, Nanotechnology, 18, 325602 (2007)