화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korea-Australia Rheology Journal, Vol.21, No.2, 127-133, June, 2009
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Storage of laboratory animal blood samples causes hemorheological alterations : Inter-species differences and the effects of duration and temperature
Norbert Nemeth†, Oguz K. Baskurt1, Herbert J. Meiselman2, Ferenc Kiss, Mehmet Uyuklu1, Timea Hever, Erika Sajtos, Peter Kenyeres3, Kalman Toth3, Istvan Furka, and Iren Miko
  • Department of Operative Techniques and Surgical Research, Institute of Surgery,Medical and Health Science Center, University of Debrecen, Hungary
  • 1Department of Physiology, Akdeniz University, Faculty of Medicine, Antalya, Turkey
  • 2Department of Physiology and Biophysics, University of Southern California Keck School of Medicine, Los Angeles, CA, USA
  • 3First Department of Medicine, University of Pecs, Hungary
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Hemorheological results may be influenced by the time between blood sampling and measurement, and storage conditions (e.g., temperature, time) during sample delivery between laboratories may further affect the resulting data. This study examined possible hemorheological alterations subsequent to storage of rat and dog blood at room temperature (22 ℃) or with cooling (4~10 ℃) for 2, 4, 6, 24, 48 and 72 hours. Measured hemorheological parameters included hematological indices, RBC aggregation and RBC deformability. Our results indicate that marked changes of RBC deformability and of RBC aggregation in whole blood can occur during storage, especially for samples stored at room temperature. The patterns of deformability and aggregation changes at room temperature are complex and species specific, whereas those for storage at the lower temperature range are much less complicated. For room temperature storage, it thus seems logical to suggest measuring rat and dog cell deformability within 6 hours; aggregation should be measured immediately for rat blood or within 6 hours for dog blood. Storage at lower temperatures allows measuring EI up to 72 hours after sampling, while aggregation must be measured immediately, or if willing to accept a constant decrease, over 24~72 hours.
Keywords:in vitro time;blood storage;hemorheology;rat;dog
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