생분해성 지방족 폴리에스테르에 관한 연구(II): Copolyesterethylene의 생분해성 평가

강혜정; 박태욱; 임윤택

Polymer(Korea), Vol.20, No.6, 960-970, November, 1996

Export Citation

생분해성 지방족 폴리에스테르에 관한 연구(II): Copolyesterethylene의 생분해성 평가

Biodegradable Aliphatic Polyester (Ⅱ) : Evaluation of Biodegradability of Copolyesterethylene

강혜정, 박태욱, 임윤택

초록

합성한 aliphatic polyester인 copolyesterethylene (CPEE)의 생분해성을 토양미생물, 효소 및 실험실적 토양매립시험 (pot test)에 의해 평가하였다. CPEE의 생분해성을 평가하기 위하여 CPEE에 대해 친화성을 갖는 4종의 미생물을 토양으로부터 분리하였다. 이들 4종균주의 미생물학적 특성을 비교, 동정한 결과 각각 Aspergillus Fumigatus, Aspergillus terreus, Penicillium olsonii 및 Fusarium semitectum인 것으로 알려졌으며 이들 미생물의 enzyme activity는 pH 6∼8 범위에서 증가하는 것으로 나타났다. 이들 미생물에 의한 CPEE의 분해성 평가결과 분해율은 각 균주별로 차이가 있었으나 8주 후 70∼95%의 중량감소율을 나타내었다. 또한 실험실적 토양매립에 의한 분해성 평가결과 6개월 후 약 11%의 중량감소와 함께 분자량 감소를 보여주었으며 Rhizopus Arrihzus lipase를 이용한 효소에 의한 분해성 평가결과 48시간 후 35.5%의 분해율을 나타내었다.

Biodegradability of the aliphatic polyester synthesized, copolyesterethylene (CPFE), was Investigated by the microorganism and enzyme treatment and the laboratory soil burial test (pot test). In order to evaluate the biodegradation of the CPEE, four kinds of microorganisms which have the affinity for CPEE were isolated from soil. These microorganisms were microbiologically identified as Aspergillus Fumigatus, Aspergillus terreus, Penicillum olsonii and Fusarium semitectum, respectively and showed an increase in the enzyme activity in the range of pH 6∼8. With the microorganism treatment, the rate of biodegradation in terms of weight loss was 70∼95%, depending on the kind of microorganism treated. Also the laboratory soil burial test showed about 11% weight loss and a decrease in the molecular weight of CPEE after 6 months of exposure to microbe present in the soil. With lipase (Rhizopus Arrihzus) treatment, the rate of biodegradation of CPEE was about 35% after 48 hrs of degradation.

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[3] Schnabel W, "Polymer Degradation: Principles and Practical Applications," Hansen International, Munchen (1981)

[4] Kawai F, CRC Critical Rev. Biotech., 6, 723 (1987)

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[6] Deng XM, Xiong CD, Cheng LM, Xu XP, J. Polym. Sci. C: Polym. Lett., 28, 411 (1990)

[7] Tanio T, Fukui T, Shirakura Y, Saito T, Tomita K, Kaiho T, Masamune S, Eur. J. Biochem., 124, 71 (1982)

[8] Tokiwa Y, Ando T, Suzuki T, J. Ferment. Technol., 54, 603 (1976)

[9] Otake Y, Kobayashi T, Gomi Y, Ito S, Hyakutake K, J. Jpn. Rubber Soc., 64, 688 (1991)

[10] Albertsson AC, Banhidi ZG, J. Appl. Polym. Sci., 25, 1655 (1980)

[11] Kang HJ, Park TW, Kim YJ, Lee CG, Polym.(Korea), submitted

[12] Domsch KH, Gams W, Anderson TH, "Compendium of Soil Fungi," vol. 1, Academic Press, London (1980)

[13] Samson RA, Hoekstra ES, VanOorschot CAN, "Introduction to Food-Borne Fungi," Centraalbureau Voor Schimmelcultures, Baarn (1981)

[14] Fassatiova O, "Moulds and Filamentous Fungi in Technical Microbiology," Elsevier, Amsterdam (1986)

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[21] Tokiwa Y, Suzuki T, Agric. Biol. Chem., 41, 265 (1977)

[22] Samad Y, J. Microbiol. Methods, 9, 51 (1989)

[23] Tokiwa Y, Suzuki T, Takeda K, Agric. Biol. Chem., 52, 1937 (1988)

[24] Yamamoto N, Nakayama A, Hayashi K, Iyoda J, Polym. Prepr., 39(7), 2301 (1990)