Overexpression of ethionine resistance gene for maximized production of S-adenosylmethionine in Saccharomyces cerevisiae sake kyokai No. 6

Sang-Woo Lee; Bu-Soo Park; Eun-Sil Choi; Min-Kyu Oh

Korean Journal of Chemical Engineering, Vol.27, No.2, 587-589, February, 2010

DOI10.1007/s11814-010-0100-3 Export Citation

Overexpression of ethionine resistance gene for maximized production of S-adenosylmethionine in Saccharomyces cerevisiae sake kyokai No. 6

Sang-Woo Lee, Bu-Soo Park¹, Eun-Sil Choi, and Min-Kyu Oh^†

Department of Chemical & Biological Engineering, Korea University, 5-1 Anam-dong, Sungbuk-gu, Seoul 136-713, Korea
¹Samyang Genex, 63-2 Hwaam-dong, Yuseong-gu, Daejeon 305-717, Korea

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In our previous work, we developed an auxotrophic mutant of Saccharomyces cerevisiae sake kyokai No. 6 strain for the selection of recombinant DNA. Sake yeast can accumulate high amount of S-adenosylmethionine (SAM) that is effective for treating depression, Alzheimer’s disease and so on. In this work, we further introduced SAM2 gene and ERC1, a frame-shift mutant of YHR032w, to maximize the production of S-adenosylmethionine in S. cerevisiae sake K6. The recombinant strain accumulated SAM about half of its dry cell weight.

Keywords:S-adenosyl-L-methionine;Saccharomyces cerevisiae sake;Metabolic Engineering

[References]

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Roje S, Wang H, McNeil SD, Raymond RK, Appling DR, Shachar-Hill Y, Bohnert HJ, Hanson AD, J. Biol. Chem., 274, 36089 (1999)

[Related Articles]

[1] Cantoni GL, Annu. Rev. Biochem., 44, 435 (1975)

[2] Lu SC, Int. J. Biochem. Cell. Biol., 32, 391 (2000)

[3] Mato JM, Corrales FJ, Lu SC, Avila MA, Faseb J., 16, 15 (2002)

[4] Mischoulon D, Fava M, Am. J. Clin. Nutr., 76, 1158S (2002)

[5] Fuso A, Seminara L, Cavallaro RA, D’Anselmi F, Scarpa S, Mol. Cell. Neurosci., 28, 195 (2005)

[6] Roje S, Wang H, McNeil SD, Raymond RK, Appling DR, Shachar-Hill Y, Bohnert HJ, Hanson, AD, J. Biol. Chem., 274, 36089 (1999)

[7] Choi ES, Park BS, Lee SW, Oh MK, Korean J. Chem. Eng., 26(1), 156 (2009)

[8] Shiomi N, Fukuda H, Fukuda Y, Murata K, Kimura A, J. Ferment. Bioeng., 71, 211 (1991)

[9] Shobayashi M, Ukena E, Fujii T, Iefuji H, Biosci. Biotechnol. Biochem., 71, 323 (2007)

[10] Budovskaya YV, Stephan JS, Deminoff SJ, Herman PK, Proc. Natl. Acad. Sci. USA, 102, 13933 (2005)

[11] Sreenivas A, Carman GM, J. Biol. Chem., 278, 20673 (2003)

[12] Higuchi R, Krummel B, Saiki RK, Nucleic Acids Res., 16, 7351 (1988)

[13] Schwencke J, De Robichon-Szulmajster H, Eur. J. Biochem., 65, 49 (1976)

[14] Roje S, Wang H, McNeil SD, Raymond RK, Appling DR, Shachar-Hill Y, Bohnert HJ, Hanson AD, J. Biol. Chem., 274, 36089 (1999)