Taxol, a powerful anti-cancer agent, was extracted and purified from yew tree by solvents. The solvent used in extraction was methanol, and the extracts was further concentrated by the partition of water and methylene chloride. Such impurities as lipid, chlorophyl, pigment, et al. were removed in the pretreatment step with open column, where silica packings were packed. The extracts 10 ml passed through the column with mobile phase, and the effluents were collected by the bands developed in the column. The solvents used were hexane, methylene chloride, chloroform, acetone, methanol, ethanol, IPA, and 1-propanol. They were used as mobile phase with a single or binary combination. From the experimental results, the mobile phase of MC/acetone most clearly showed the developed band of the extract, and the elution time was fasted by the gradient mode(75/25 after 97/3 vol.% of MC/acetone).